The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
The capacitating agent bicarbonate induces protein kinase A-dependent changes in phospholipid transbilayer behavior in the sperm plasma membrane
Abstract
A flow cytometric procedure was used to follow the effect of bicarbonate, a key inducer of sperm capacitation in vitro, on the transbilayer behavior of C6NBD-phospholipids in the plasma membrane of living acrosome-intact boar spermatozoa under physiological conditions. In the absence of bicarbonate, 97% of C6NBD-phosphatidylserine and 78% of C6NBD-phosphatidylethanolamine was rapidly translocated from the outer leaflet to the inner, whereas relatively little C6NBD-phosphatidylcholine and C6NBD-sphingomyelin was translocated (15% and 5%, respectively). Inclusion of 15 mM bicarbonate/5%CO2 markedly slowed down the rates of translocation of the aminophospholipids without altering their final distribution, whereas it increased the proportions of C6NBD-phosphatidylcholine and C6NBD-sphingomyelin translocated (30% and 20%, respectively). Bicarbonate activated very markedly the outward translocation of all four phospholipid classes. The changes in C6NBD-phospholipid behavior were accompanied by increased membrane lipid disorder as detected by merocyanine 540, and also by increased potential for phospholipase catabolism of the C6NBD-phospholipid probes. All three changes were mediated via a cAMP-dependent protein phosphorylation pathway. We suspect that the changes result from an activation of the non-specific bidirectional translocase (‘scramblase’). They have important implications with respect to sperm fertilizing function.
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Formation and function of phosphatidylserine and phosphatidylethanolamine in mammalian cells
Vance, J. E., & Tasseva, G. (2013) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2012.08.016 · Cited by 521
Metabolism and functions of phosphatidylserine
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Getting to the Outer Leaflet: Physiology of Phosphatidylserine Exposure at the Plasma Membrane
Bevers, E. M., & Williamson, P. L. (2016) · Physiological Reviews · doi:10.1152/physrev.00020.2015 · Cited by 447
Novel signaling pathways involved in sperm acquisition of fertilizing capacity
Visconti, P. E., Westbrook, V. A., Chertihin, O., Demarco, I., Sleight, S., & Diekman, A. B. (2002) · Journal of Reproductive Immunology · doi:10.1016/s0165-0378(01)00103-6 · Cited by 342
Ion channels, phosphorylation and mammalian sperm capacitation
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Flesch, F. M., Brouwers, J. F. H. M., Nievelstein, P. F. E. M., Verkleij, A. J., van Golde, L. M. G., Colenbrander, B., & Gadella, B. M. (2001) · Journal of Cell Science · doi:10.1242/jcs.114.19.3543 · Cited by 296
Self-citationFlow cytometric evaluation of sperm parameters in relation to fertility potential
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Gadella, B. M., & Harrison, R. A. P. (2002) · Biology of Reproduction · doi:10.1095/biolreprod67.1.340 · Cited by 263
Self-citationMolecular Basis of Human Sperm Capacitation
Puga Molina, L. C., Luque, G. M., Balestrini, P. A., Marín-Briggiler, C. I., Romarowski, A., & Buffone, M. G. (2018) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2018.00072 · Cited by 256
Interplay Between Endocannabinoids, Steroids and Cytokines in the Control of Human Reproduction
Battista, N., Pasquariello, N., Di Tommaso, M., & Maccarrone, M. (2008) · Journal of Neuroendocrinology · doi:10.1111/j.1365-2826.2008.01684.x · Cited by 144
Metadata
- Authors
- Gadella, B. M., & Harrison, R. A. P.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2000-06-01
- Volume
- 127
- Issue
- 11
- Pages
- 2407-2420
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W1842647761
- Cited by
- 297
- References
- 29
- FWCI
- 4.51
- Type
- journal article
- Language
- en
- Open access
- closed
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