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Conotruncal myocardium arises from a secondary heart field
Abstract
The primary heart tube is an endocardial tube, ensheathed by myocardial cells, that develops from bilateral primary heart fields located in the lateral plate mesoderm. Earlier mapping studies of the heart fields performed in whole embryo cultures indicate that all of the myocardium of the developed heart originates from the primary heart fields. In contrast, marking experiments in ovo suggest that the atrioventricular canal, atria and conotruncus are added secondarily to the straight heart tube during looping. The results we present resolve this issue by showing that the heart tube elongates during looping, concomitant with accretion of new myocardium. The atria are added progressively from the caudal primary heart fields bilaterally, while the myocardium of the conotruncus is elongated from a midline secondary heart field of splanchnic mesoderm beneath the floor of the foregut. Cells in the secondary heart field express Nkx2.5 and Gata-4, as do the cells of the primary heart fields. Induction of myocardium appears to be unnecessary at the inflow pole, while it occurs at the outflow pole of the heart. Accretion of myocardium at the junction of the inflow myocardium with dorsal mesocardium is completed at stage 12 and later (stage 18) from the secondary heart field just caudal to the outflow tract. Induction of myocardium appears to move in a caudal direction as the outflow tract translocates caudally relative to the pharyngeal arches. As the cells in the secondary heart field begin to move into the outflow or inflow myocardium,they express HNK-1 initially and then MF-20, a marker for myosin heavy chain. FGF-8 and BMP-2 are present in the ventral pharynx and secondary heart field/outflow myocardium, respectively, and appear to effect induction of the cells in a manner that mimics induction of the primary myocardium from the primary heart fields. Neither FGF-8 nor BMP-2 is present as inflow myocardium is added from the primary heart fields. The addition of a secondary myocardium to the primary heart tube provides a new framework for understanding several null mutations in mice that cause defective heart development.
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Cited by
Cai, C.-L., Liang, X., Shi, Y., Chu, P.-H., Pfaff, S. L., Chen, J., & Evans, S. (2003) · Developmental Cell · doi:10.1016/s1534-5807(03)00363-0 · Cited by 1,610
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Buckingham, M., Meilhac, S., & Zaffran, S. (2005) · Nature Reviews Genetics · doi:10.1038/nrg1710 · Cited by 1,204
Moretti, A., Caron, L., Nakano, A., Lam, J. T., Bernshausen, A., Chen, Y., Qyang, Y., Bu, L., Sasaki, M., Martin-Puig, S., et al. (2006) · Cell · doi:10.1016/j.cell.2006.10.029 · Cited by 1,038
Kattman, S. J., Huber, T. L., & Keller, G. M. (2006) · Developmental Cell · doi:10.1016/j.devcel.2006.10.002 · Cited by 729
Verzi, M. P., McCulley, D. J., De Val, S., Dodou, E., & Black, B. L. (2005) · Developmental Biology · doi:10.1016/j.ydbio.2005.08.041 · Cited by 527
Prall, O. W. J., Menon, M. K., Solloway, M. J., Watanabe, Y., Zaffran, S., Bajolle, F., Biben, C., McBride, J. J., Robertson, B. R., Chaulet, H., et al. (2007) · Cell · doi:10.1016/j.cell.2007.01.042 · Cited by 517
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Harvey, R. P. (2002) · Nature Reviews Genetics · doi:10.1038/nrg843 · Cited by 450
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Gessert, S., & Kühl, M. (2010) · Circulation Research · doi:10.1161/circresaha.110.221531 · Cited by 414
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Noden, D. M., & Francis‐West, P. (2006) · Developmental Dynamics · doi:10.1002/dvdy.20697 · Cited by 393
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Person, A. D., Klewer, S. E., & Runyan, R. B. (2005) · International Review of Cytology · doi:10.1016/s0074-7696(05)43005-3 · Cited by 372
Sun, Y., Liang, X., Najafi, N., Cass, M., Lin, L., Cai, C.-L., Chen, J., & Evans, S. M. (2007) · Developmental Biology · doi:10.1016/j.ydbio.2006.12.048 · Cited by 332
Vitelli, F. (2002) · Human Molecular Genetics · doi:10.1093/hmg/11.8.915 · Cited by 330
Qyang, Y., Martin-Puig, S., Chiravuri, M., Chen, S., Xu, H., Bu, L., Jiang, X., Lin, L., Granger, A., Moretti, A., et al. (2007) · Cell Stem Cell · doi:10.1016/j.stem.2007.05.018 · Cited by 310
Distinct roles of Wnt/β-catenin and Bmp signaling during early cardiogenesis
Klaus, A., Saga, Y., Taketo, M. M., Tzahor, E., & Birchmeier, W. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0703113104 · Cited by 278
The anterior heart-forming field: voyage to the arterial pole of the heart
Kelly, R. G., & Buckingham, M. E. (2002) · Trends in Genetics · doi:10.1016/s0168-9525(02)02642-2 · Cited by 278
Myocardial Lineage Development
Evans, S. M., Yelon, D., Conlon, F. L., & Kirby, M. L. (2010) · Circulation Research · doi:10.1161/circresaha.110.227405 · Cited by 277
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Zeisberg, E. M., Ma, Q., Juraszek, A. L., Moses, K., Schwartz, R. J., Izumo, S., & Pu, W. T. (2005) · Journal of Clinical Investigation · doi:10.1172/jci23769 · Cited by 262
The role of secondary heart field in cardiac development
Dyer, L. A., & Kirby, M. L. (2009) · Developmental Biology · doi:10.1016/j.ydbio.2009.10.009 · Cited by 261
Self-citationEmbryonic Heart Progenitors and Cardiogenesis
Brade, T., Pane, L. S., Moretti, A., Chien, K. R., & Laugwitz, K.-L. (2013) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a013847 · Cited by 254
Required, tissue-specific roles for Fgf8 in outflow tract formation and remodeling
Park, E. J., Ogden, L. A., Talbot, A., Evans, S., Cai, C.-L., Black, B. L., Frank, D. U., & Moon, A. M. (2006) · Development · doi:10.1242/dev.02367 · Cited by 254
The anatomical components of the cardiac outflow tract of chondrichthyans and actinopterygians
Lorenzale, M., López‐Unzu, M. A., Rodríguez, C., Fernández, B., Durán, A. C., & Sans‐Coma, V. (2018) · Biological Reviews · doi:10.1111/brv.12411 · Cited by 20
Metadata
- Authors
- Waldo, K. L., Kumiski, D. H., Wallis, K. T., Stadt, H. A., Hutson, M. R., Platt, D. H., & Kirby, M. L.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2001-08-15
- Volume
- 128
- Issue
- 16
- Pages
- 3179-3188
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W1931060259
- Cited by
- 650
- References
- 0
- FWCI
- 9.34
- Type
- journal article
- Language
- en
- Open access
- closed
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