Cited by 85Wnt/β-catenin signaling in development and cancerMicrotubule and mitosis dynamicsHippo pathway signaling and YAP/TAZ

AMER1 regulates the distribution of the tumor suppressor APC between microtubules and the plasma membrane

Grohmann, Annette, Tanneberger, Kristina, Alzner, Astrid, Schneikert, Jean, Behrens, Jürgen·2007·Journal of Cell Science

Abstract

APC is a multifunctional tumor suppressor protein that negatively controls Wnt signaling, but also regulates cell adhesion and migration by interacting with the plasma membrane and the microtubule cytoskeleton. Although the molecular basis for the microtubule association of APC is well understood, molecular mechanisms that underlie its plasma membrane localization have remained elusive. We show here that APC is recruited to the plasma membrane by binding to APC membrane recruitment 1 (AMER1), a novel membrane-associated protein that interacts with the ARM repeat domain of APC. The N-terminus of AMER1 contains two distinct phosphatidylinositol(4,5)-bisphosphate [PtdIns(4,5)P2]-binding domains, which mediate its localization to the plasma membrane. Overexpression of AMER1 increases APC levels and redirects APC from microtubule ends to the plasma membrane of epithelial cells. Conversely, siRNA-mediated knockdown of AMER1 reduces the overall levels of APC, promotes its association with microtubule ends in cellular protrusions and disturbs intercellular junctions. These data indicate that AMER1 controls the subcellular distribution of APC between membrane- and microtubule-associated pools, and might thereby regulate APC-dependent cellular morphogenesis, cell migration and cell-cell adhesion.

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Crossref · 10.1242/jcs.011320

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Authors
Grohmann, A., Tanneberger, K., Alzner, A., Schneikert, J., & Behrens, J.
Affiliations
  • Friedrich-Alexander-Universität Erlangen-Nürnberg (DE)
  • Nikolaus-Fiebiger-Center for Molecular Medicine, University Erlangen-Nuremberg, Glückstr. 6, 91054 Erlangen, Germany
Venue
Journal of Cell Science
Published
2007-11-01
Volume
120
Issue
21
Pages
3738-3747
ISSN
  • 0021-9533 (ISSN-L)
  • 1477-9137
OpenAlex
W2108699597
Cited by
85
References
43
Citations by year
2026: 1 · 2025: 3 · 2024: 6 · 2023: 1 · 2022: 3 · 2021: 4 · 2020: 3 · 2019: 6 · 2018: 3 · 2017: 4 · 2015: 4 · 2014: 3 · 2013: 6 · 2012: 8
FWCI
2.25
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Cell Science (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Hippo pathway signaling and YAP/TAZ
Keywords
AMER1, APC tumor suppressor, microtubules, plasma membrane localization, subcellular localization, epithelial cells, cell migration, cell-cell adhesion, intercellular junctions, Wnt signaling, cell protrusions
MeSH terms
Amino Acid Sequence, Animals, Cell Membrane, Humans, Membrane Proteins, Microtubules, Molecular Sequence Data, Sequence Alignment, Two-Hybrid System Techniques, Tumor Suppressor Proteins, Adenomatous Polyposis Coli Protein, Cell Line, Tumor, Adaptor Proteins, Signal Transducing

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