Analysis of GTPase-activating proteins: Rab1 and Rab43 are key Rabs required to maintain a functional Golgi complex in human cells
Abstract
Rab GTPases control vesicle movement and tethering membrane events in membrane trafficking. We used the 38 human Rab GTPase activating proteins (GAPs) to identify which of the 60 Rabs encoded in the human genome function at the Golgi complex. Surprisingly, this screen identified only two GAPs, RN-tre and TBC1D20, disrupting both Golgi organization and protein transport. RN-tre is the GAP for Rab43, and controls retrograde transport into the Golgi from the endocytic pathway. TBC1D20 is the ER-localized GAP for Rab1, and is the only GAP blocking the delivery of secretory cargo from the ER to the cell surface. Strikingly, its expression causes the loss of the Golgi complex, highlighting the importance of Rab1 for Golgi biogenesis. These effects can be antagonized by reticulon, a binding partner for TBC1D20 in the ER. Together, these findings indicate that Rab1 and Rab43 are key Rabs required for the biogenesis and maintenance of a functional Golgi structure, and suggest that other Rabs acting at the Golgi complex are likely to be functionally redundant.
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The parasite Toxoplasma sequesters diverse Rab host vesicles within an intravacuolar network
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Rab33b and Rab6 are Functionally Overlapping Regulators of Golgi Homeostasis and Trafficking
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Protein LidA from Legionella is a Rab GTPase supereffector
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The Rab family of proteins: 25 years on
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Defects in the COG complex and COG-related trafficking regulators affect neuronal Golgi function
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Multiple Types of Guanine Nucleotide Exchange Factors (GEFs) for Rab Small GTPases
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Intermediate compartment (IC): from pre-Golgi vacuoles to a semi-autonomous membrane system
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Mechanism of Rab1b deactivation by the Legionella pneumophila GAP LepB
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RUTBC1 Protein, a Rab9A Effector That Activates GTP Hydrolysis by Rab32 and Rab33B Proteins
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Self-citationEvolution of Tre-2/Bub2/Cdc16 (TBC) Rab GTPase-activating proteins
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Rab proteins of the endoplasmic reticulum: functions and interactors
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Warburg Micro syndrome is caused by RAB18 deficiency or dysregulation
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AMPylation Is Critical for Rab1 Localization to Vacuoles Containing Legionella pneumophila
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Autophagy: A Double-Edged Sword in Male Reproduction
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The GTPase-Activating Protein RN-tre Controls Focal Adhesion Turnover and Cell Migration
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The GTPase Rab43 Controls the Anterograde ER-Golgi Trafficking and Sorting of GPCRs
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- Authors
- Haas, A. K., Yoshimura, S.-I., Stephens, D. J., Preisinger, C., Fuchs, E., & Barr, F. A.
- Affiliations
- University of Liverpool (GB)
- University of Bristol (GB)
- Max Planck Institute of Biochemistry (DE)
- Corresponding author
- Barr, Francis A.
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 2007-09-01
- Volume
- 120
- Issue
- 17
- Pages
- 2997-3010
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W2064782801
- Cited by
- 201
- References
- 56
- Citations by year
- 2025: 8 · 2024: 5 · 2023: 8 · 2022: 7 · 2021: 11 · 2020: 8 · 2019: 10 · 2018: 9 · 2017: 10 · 2016: 16 · 2015: 9 · 2014: 13 · 2013: 15 · 2012: 21
- FWCI
- 4.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Cell Science (publishedVersion)
- PubMed (publishedVersion)
- Explore Bristol Research (publishedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- Bristol Research (University of Bristol) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
- Keywords
- Rab GTPases, Rab1, Rab GTPase-activating protein, reticulons, retrograde transport, secretory pathway, ER-Golgi transport, membrane trafficking, functional redundancy
- MeSH terms
- rab11 GTP-Binding Proteins, Animals, Biological Transport, Endoplasmic Reticulum, Golgi Apparatus, HeLa Cells, Humans, Nerve Tissue Proteins, Phenotype, Recombinant Fusion Proteins, Genome, Human, rab GTP-Binding Proteins, rab1 GTP-Binding Proteins, Two-Hybrid System Techniques, RNA Interference
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