CoChat
Sign Up
Cited by 345

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

SIRT2 regulates NF-κB dependent gene expression through deacetylation of p65 Lys310

Rothgiesser, Karin M., Erener, Süheda, Waibel, Susanne, Lüscher, Bernhard, Hottiger, Michael O.·2010·Journal of Cell Science
DOI 10.1242/jcs.073783
Open this paper in CoChat — free account

Abstract

NF-κB regulates the expression of a large number of target genes involved in the immune and inflammatory response, apoptosis, cell proliferation, differentiation and survival. In this study, we identified SIRT2 as a deacetylase of the transcription factor p65. SIRT2 is a member of the family of sirtuins, which are NAD+-dependent deacetylases involved in several cellular processes. SIRT2 interacts with p65 in the cytoplasm and deacetylates p65 in vitro and in vivo at Lys310. Moreover, p65 is hyperacetylated at Lys310 in Sirt2−/− cells after TNFα stimulation, which results in the increase in expression of a subset of p65 acetylation-dependent target genes. Our work provides evidence that p65 is deacetylated by SIRT2 in the cytoplasm to regulate the expression of specific NF-κB-dependent genes.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1242/jcs.073783

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • SIRT2 and SIRT6 may down-regulate the inflammatory response through deacetylation of NF-kB subunits and transcriptional repression of their target genes.

    “SIRT2 and SIRT6 may also down-regulate the inflammatory response through deacetylation of NF-kB subunits and transcriptional repression of their target genes”

    From The Hallmarks of Aging (2013) · cites this paper as Rothgiesser et al., 2010

    The Abstract and the section on SIRT2-dependent gene expression report that SIRT2 deacetylates the NF-κB subunit p65 and limits expression of a subset of its target genes. The Discussion explicitly says SIRT6 does not deacetylate p65; it describes SIRT6 as attenuating NF-κB signaling by deacetylating histone H3K9 instead. Thus the proposed mechanism is supported for SIRT2, not SIRT6. (LLM, conf=0.90)

    Experimental — accuracy not yet measured

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 35

  1. Beg, 1996, An essential role for NF-kappaB in preventing TNF-alpha-induced cell death, Science, 274, 782, 10.1126/science.274.5288.782 · DOI · Paper page
  2. Black, 2008, The SIRT2 deacetylase regulates autoacetylation of p300, Mol. Cell, 32, 449, 10.1016/j.molcel.2008.09.018 · DOI
  3. Bonizzi, 2004, Activation of IKKalpha target genes depends on recognition of specific kappaB binding sites by RelB:p52 dimers, EMBO J., 23, 4202, 10.1038/sj.emboj.7600391 · DOI
  4. Buerki, 2008, Functional relevance of novel p300-mediated lysine 314 and 315 acetylation of RelA/p65, Nucleic Acids Res., 36, 1665, 10.1093/nar/gkn003 · DOI
  5. Chen, 2001, Duration of nuclear NF-kappaB action regulated by reversible acetylation, Science, 293, 1653, 10.1126/science.1062374 · DOI
  6. Cheng, 2003, Developmental defects and p53 hyperacetylation in Sir2 homolog (SIRT1)-deficient mice, Proc. Natl. Acad. Sci. USA, 100, 10794, 10.1073/pnas.1934713100 · DOI · Paper page
  7. Denu, 2003, Linking chromatin function with metabolic networks: Sir2 family of NAD(+)-dependent deacetylases, Trends Biochem. Sci., 28, 41, 10.1016/S0968-0004(02)00005-1 · DOI
  8. Dryden, 2003, Role for human SIRT2 NAD-dependent deacetylase activity in control of mitotic exit in the cell cycle, Mol. Cell. Biol., 23, 3173, 10.1128/MCB.23.9.3173-3185.2003 · DOI
  9. Ghosh, 1998, NF-kappa B and Rel proteins: evolutionarily conserved mediators of immune responses, Annu. Rev. Immunol., 16, 225, 10.1146/annurev.immunol.16.1.225 · DOI
  10. Hayden, 2008, Shared principles in NF-kappaB signaling, Cell, 132, 344, 10.1016/j.cell.2008.01.020 · DOI
  11. Heltweg, 2006, Antitumor activity of a small-molecule inhibitor of human silent information regulator 2 enzymes, Cancer Res., 66, 4368, 10.1158/0008-5472.CAN-05-3617 · DOI
  12. Hoffmann, 2006, Circuitry of nuclear factor kappaB signaling, Immunol. Rev., 210, 171, 10.1111/j.0105-2896.2006.00375.x · DOI · Paper page
  13. Hoffmann, 2002, The IkappaB-NF-kappaB signaling module: temporal control and selective gene activation, Science, 298, 1241, 10.1126/science.1071914 · DOI
  14. Huang, 2008, Brd4 coactivates transcriptional activation of NF-{kappa}B via specific binding to acetylated RelA, Mol. Cell. Biol, 29, 1375, 10.1128/MCB.01365-08 · DOI
  15. Huang, 2000, A nuclear export signal in the N-terminal regulatory domain of IkappaBalpha controls cytoplasmic localization of inactive NF-kappaB/IkappaBalpha complexes, Proc. Natl. Acad. Sci. USA, 97, 1014, 10.1073/pnas.97.3.1014 · DOI
  16. Kawahara, 2009, SIRT6 links histone H3 lysine 9 deacetylation to NF-kappaB-dependent gene expression and organismal life span, Cell, 136, 62, 10.1016/j.cell.2008.10.052 · DOI · Paper page
  17. Kiernan, 2003, Post-activation turn-off of NF-kappa B-dependent transcription is regulated by acetylation of p65, J. Biol. Chem., 278, 2758, 10.1074/jbc.M209572200 · DOI
  18. Michishita, 2005, Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins, Mol. Biol. Cell, 16, 4623, 10.1091/mbc.e05-01-0033 · DOI · Paper page
  19. Moreno, 2010, Specification of the NF-{kappa}B transcriptional response by p65 phosphorylation and TNF-induced nuclear translocation of IKK{varepsilon}, Nucleic Acids Res, 38, 6029, 10.1093/nar/gkq439 · DOI
  20. North, 2007, Interphase nucleo-cytoplasmic shuttling and localization of SIRT2 during mitosis, PLoS ONE, 2, e784, 10.1371/journal.pone.0000784 · DOI
  21. North, 2003, The human Sir2 ortholog, SIRT2, is an NAD+-dependent tubulin deacetylase, Mol. Cell, 11, 437, 10.1016/S1097-2765(03)00038-8 · DOI
  22. Pandithage, 2008, The regulation of SIRT2 function by cyclin-dependent kinases affects cell motility, J. Cell Biol., 180, 915, 10.1083/jcb.200707126 · DOI
  23. Perez, 1995, Overexpression of RelA in transgenic mouse thymocytes: specific increase in levels of the inhibitor protein I kappa B alpha, Mol. Cell. Biol., 15, 3523, 10.1128/MCB.15.7.3523 · DOI
  24. Perkins, 1997, Regulation of NF-kappaB by cyclin-dependent kinases associated with the p300 coactivator, Science, 275, 523, 10.1126/science.275.5299.523 · DOI
  25. Perrod, 2001, A cytosolic NAD-dependent deacetylase, Hst2p, can modulate nucleolar and telomeric silencing in yeast, EMBO J., 20, 197, 10.1093/emboj/20.1.197 · DOI
  26. Pfaffl, 2002, Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR, Nucleic Acids Res., 30, e36, 10.1093/nar/30.9.e36 · DOI
  27. Polager, 2002, E2Fs up-regulate expression of genes involved in DNA replication, DNA repair and mitosis, Oncogene, 21, 437, 10.1038/sj.onc.1205102 · DOI
  28. Rothgiesser, 2010, Acetylation of p65 at lysine 314 is important for late NF-kappaB-dependent gene expression, BMC Genomics, 11, 22, 10.1186/1471-2164-11-22 · DOI
  29. Sauve, 2006, The biochemistry of sirtuins, Annu. Rev. Biochem., 75, 435, 10.1146/annurev.biochem.74.082803.133500 · DOI · Paper page
  30. Sterner, 2000, Acetylation of histones and transcription-related factors, Microbiol. Mol. Biol. Rev., 64, 435, 10.1128/MMBR.64.2.435-459.2000 · DOI
  31. Taylor, 2008, Biological and potential therapeutic roles of sirtuin deacetylases, Cell. Mol. Life Sci., 65, 4000, 10.1007/s00018-008-8357-y · DOI
  32. Vaquero, 2006, SirT2 is a histone deacetylase with preference for histone H4 Lys 16 during mitosis, Genes Dev., 20, 1256, 10.1101/gad.1412706 · DOI
  33. Westphal, 2007, A therapeutic role for sirtuins in diseases of aging?, Trends Biochem. Sci., 32, 555, 10.1016/j.tibs.2007.09.008 · DOI
  34. Yang, 2007, HATs and HDACs: from structure, function and regulation to novel strategies for therapy and prevention, Oncogene, 26, 5310, 10.1038/sj.onc.1210599 · DOI · Paper page
  35. Zhong, 2002, The phosphorylation status of nuclear NF-kappa B determines its association with CBP/p300 or HDAC-1, Mol. Cell, 9, 625, 10.1016/S1097-2765(02)00477-X · DOI

Cited by

Sorted by citations

Metadata

Authors
Rothgiesser, K. M., Erener, S., Waibel, S., Lüscher, B., & Hottiger, M. O.
Affiliations
  • Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Winterthurerstraße 190, 8057 Zurich, Switzerland
  • Institut für Biochemie und Molekularbiologie, Medical School, RWTH Aachen University, Pauwelsstraße 30, 52074 Aachen, Germany
Venue
Journal of Cell Science
Publisher
The Company of Biologists
Published
2010-12-15
Volume
123
Issue
24
Pages
4251-4258
ISSN
  • 1477-9137
  • 0021-9533
DOI
10.1242/jcs.073783
Cited by
345
References
5
Language
en

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review