The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
The regulation of bidirectional mitochondrial transport is coordinated with axonal outgrowth
Abstract
Although small molecules such as ATP diffuse freely in the cytosol, many types of cells nonetheless position their mitochondria in regions of intense ATP consumption. We reasoned that in the highly elongated axonal processes of growing neurons in culture, the active growth cone would form a focus of ATP consumption so distant from the cell body as to require the positioning of mitochondria nearby via regulated axonal transport. To test this hypothesis, we quantified the distribution and transport behavior of mitochondria in live, aerobically respiring chick sympathetic neurons. We found that in the distal region of actively growing axons, the distribution of mitochondria was highly skewed toward the growth cone, with a sevenfold higher density in the region immediately adjacent to the growth cone than in the region 100 m away. When axonal outgrowth was blocked by substratum-associated barriers or mild cytochalasin E treatment, the gradient of mitochondrial distribution collapsed as mitochondria exited retrogradely from the distal region, becoming uniformly distributed along the axon within one hour. Analysis of individual mitochondrial behaviors revealed that mito-chondrial movement everywhere was bidirectional but balanced so that net transport was anterograde in growing axons and retrograde in blocked axons. This reversal in net transport derived from two separate modulations of mitochondrial movement. First, moving mitochondria underwent a transition to a persistently stationary state in the region of active growth cones that was reversed when growth cone activity was halted. Second, the fraction of time that mitochondria spent moving anterogradely was sharply reduced in non-growing axons. Together, these could account for the formation of gradients of mitochondria in growing axons and their dissipation when outgrowth was blocked. This regulated transport behavior was not dependent upon the ability of mitochondria to produce ATP. Our data indicate that mitochondria possess distinct motor activities for both directions of movement and that mitochondrial transport in axons is regulated by both recruitment between stationary and moving states, and direct regulation of the anterograde motor.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility
VALE, R., REESE, T., & SHEETZ, M. (1985) · Cell · doi:10.1016/s0092-8674(85)80099-4 · Cited by 1,993
Cited by
The pathways of mitophagy for quality control and clearance of mitochondria
Ashrafi, G., & Schwarz, T. L. (2013) · Cell Death & Differentiation · doi:10.1038/cdd.2012.81 · Cited by 1,900
The importance of dendritic mitochondria in the morphogenesis and plasticity of spines and synapses
Li, Z., Okamoto, K.-I., Hayashi, Y., & Sheng, M. (2004) · Cell · doi:10.1016/j.cell.2004.11.003 · Cited by 1,498
Functions and dysfunctions of mitochondrial dynamics
Detmer, S. A., & Chan, D. C. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2275 · Cited by 1,369
Ebneth, A., Godemann, R., Stamer, K., Illenberger, S., Trinczek, B., Mandelkow, E.-M., & Mandelkow, E. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.143.3.777 · Cited by 820
The axonal transport of mitochondria
Hollenbeck, P. J., & Saxton, W. M. (2005) · Journal of Cell Science · doi:10.1242/jcs.02745 · Cited by 771
Self-citationKang, J.-S., Tian, J.-H., Pan, P.-Y., Zald, P., Li, C., Deng, C., & Sheng, Z.-H. (2008) · Cell · doi:10.1016/j.cell.2007.11.024 · Cited by 598
Bidirectional transport along microtubules
Welte, M. A. (2004) · Current Biology · doi:10.1016/j.cub.2004.06.045 · Cited by 577
Mitochondrial Trafficking in Neurons
Schwarz, T. L. (2013) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a011304 · Cited by 525
Shepherd, G. M. G., & Harris, K. M. (1998) · The Journal of Neuroscience · doi:10.1523/jneurosci.18-20-08300.1998 · Cited by 502
Intercellular mitochondrial transfer as a means of tissue revitalization
Liu, D., Gao, Y., Liu, J., Huang, Y., Yin, J., Feng, Y., Shi, L., Meloni, B. P., Zhang, C., Zheng, M., et al. (2021) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-020-00440-z · Cited by 403
Trinczek, B., Ebneth, A., Mandelkow, E.-M., & Mandelkow, E. (1999) · Journal of Cell Science · doi:10.1242/jcs.112.14.2355 · Cited by 344
Drosophila Miro Is Required for Both Anterograde and Retrograde Axonal Mitochondrial Transport
Russo, G. J., Louie, K., Wellington, A., Macleod, G. T., Hu, F., Panchumarthi, S., & Zinsmaier, K. E. (2009) · The Journal of Neuroscience · doi:10.1523/jneurosci.5417-08.2009 · Cited by 217
Aβ-independent roles of apolipoprotein E4 in the pathogenesis of Alzheimer's disease
Huang, Y. (2010) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2010.04.004 · Cited by 170
Inter and Intracellular mitochondrial trafficking in health and disease
Shanmughapriya, S., Langford, D., & Natarajaseenivasan, K. (2020) · Ageing Research Reviews · doi:10.1016/j.arr.2020.101128 · Cited by 171
WAVE1 controls neuronal activity-induced mitochondrial distribution in dendritic spines
Sung, J. Y., Engmann, O., Teylan, M. A., Nairn, A. C., Greengard, P., & Kim, Y. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0712180105 · Cited by 117
Sirois, I., Aguilar-Mahecha, A., Lafleur, J., Fowler, E., Vu, V., Scriver, M., Buchanan, M., Chabot, C., Ramanathan, A., Balachandran, B., et al. (2019) · Molecular Cancer Research · doi:10.1158/1541-7786.mcr-19-0264 · Cited by 110
Regulation of mitochondria distribution by RhoA and formins
Minin, A. A., Kulik, A. V., Gyoeva, F. K., Li, Y., Goshima, G., & Gelfand, V. I. (2006) · Journal of Cell Science · doi:10.1242/jcs.02762 · Cited by 102
Roy, D., & Tedeschi, A. (2021) · Cells · doi:10.3390/cells10051078 · Cited by 68
Morel, M., Authelet, M., Dedecker, R., & Brion, J. P. (2010) · Neuroscience · doi:10.1016/j.neuroscience.2010.02.077 · Cited by 46
Shin, Y. S., Ryall, J. G., Britto, J. M., Lau, C. L., Devenish, R. J., Nagley, P., & Beart, P. M. (2019) · Journal of Neurochemistry · doi:10.1111/jnc.14667 · Cited by 13
Metadata
- Authors
- Morris, R. L., & Hollenbeck, P. J.
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 1993-03-01
- Volume
- 104
- Issue
- 3
- Pages
- 917-927
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W2145482520
- Cited by
- 441
- References
- 1
- FWCI
- 1.75
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
