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Loss of cytoplasmic basic fibroblast growth factor from physiologically wounded myofibers of normal and dystrophic muscle

Clarke, Mark S. F., Khakee, Robert, McNeil, Paul L.·1993·Journal of Cell Science
DOI 10.1242/jcs.106.1.121
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Abstract

Using muscle as an in vivo model system, we have tested the hypothesis that basic fibroblast growth factor is released from a cytoplasmic storage site into the extracellular environment via diffusion through survivable, mechanically-induced plasma membrane disruptions. Normal and dystrophic (mdx) mouse muscle were studied. Strong immunostaining for bFGF was detected in the cytoplasm of myofibers of uninjured muscle fixed in situ by perfusion. By contrast, myofibers did not stain cytoplasmically for bFGF after suffering lethal disruptions of their plasma membranes caused by freezing and thawing followed by sectioning. Sub-lethal, transient disruptions of myofiber plasma membranes - termed plasma membrane ‘wounds’ - were shown to be induced by needle puncture or exercise of muscle. Quantitative image analysis revealed that these wounded fibers contained significantly reduced levels of bFGF. Dystrophic exercised and unexercised muscle was found to possess an ∼6-fold higher proportion of wounded myofibers than does normal muscle under equivalent conditions. Release of bFGF at a rate that is a direct function of the frequency of myofiber wounding may explain in part how a muscle adjusts its growth to meet changing mechanical demand as well as the pathological hypertrophy characteristic of certain stages of muscular dystrophy.

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Research works printed but not linked: 67

  1. Abraham, 1986, Human basic fibroblast growth factor: nucleotide sequence and genomic organization, EMBO J, 5, 2523, 10.1002/j.1460-2075.1986.tb04530.x · DOI · Paper page
  2. Adamis, 1991, In situ injury-induced release of basic-fibroblast growth factor from corneal epithelial cells, Amer. J. Path, 139, 961
  3. Adams, 1977, Heavy metal intensification of DAB-based h P reaction product, J. Histochem. Cytochem, 29, 775, 10.1177/29.6.7252134 · DOI · Paper page
  4. Amaya, 1991, Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos, Cell, 66, 257, 10.1016/0092-8674(91)90616-7 · DOI · Paper page
  5. Anderson, 1991, Distinctive patterns of basic fibroblast growth factor (bFGF) distribution in degenerating and regenerating areas of dystrophic (mdx) striated muscles, Dev. Biol, 147, 96, 10.1016/S0012-1606(05)80010-7 · DOI
  6. Armstrong, 1983, Eccentric exercise-induced injury to rat skeletal muscle, J. Appl. Physiol, 54, 80, 10.1152/jappl.1983.54.1.80 · DOI · Paper page
  7. Baird, 1987, Fibroblast growth factors are present in the extracellular matrix produced by endothelial cells in vitro: implications for a role of heparinase-like enzymes in the neovascular response, Biochem. Biophys. Res. Commun, 142, 428, 10.1016/0006-291X(87)90292-0 · DOI · Paper page
  8. Branton, 1981, Interaction of cytoskeletal proteins on the human erythrocyte membrane, Cell, 24, 24, 10.1016/0092-8674(81)90497-9 · DOI · Paper page
  9. Broadley, 1989, Monospecific antibodies implicate basic fibroblast growth factor in normal wound repair, Lab. Invest, 61, 571
  10. Brooks, 1991, Characterization of release of basic fibroblast growth factor from bovine retinal endothelial cells in monolayer cultures, Biochem. J, 277, 113, 10.1042/bj2760113 · doi:10.1042/bj2760113
  11. Clarke, 1992, Syringe loading introduces macromolecules into living mammalian cell cytosol, J. Cell Sci, 102, 535541, 10.1242/jcs.102.3.533 · DOI
  12. Cooper, 1990, Evidence for export of a muscle lectin from cytosol to extracellular matrix and for a novel secretory mechanism, J. CellBiol, 110, 1681, 10.1083/jcb.110.5.1681 · DOI · Paper page
  13. Cordon-Cardo, 1990, Expression of basic fibroblast growth factor in normal tissues, Lab. Invest, 63, 832
  14. DiMario, 1989, Fibroblast growth factor in the extracellular matrix of dystrophic (mdx) mouse muscle, Science, 244, 688, 10.1126/science.2717945 · DOI · Paper page
  15. DiMario, 1988, Satellite cells from dystrophic (mdx) mouse muscle are stimulated by fibroblast growth factor in vitro, Differentiation, 39, 42, 10.1111/j.1432-0436.1988.tb00079.x · DOI
  16. Emery, 1988, Duchene Muscular Dystrophy
  17. Folkman, 1987, Angiogenic factors, Science, 235, 442, 10.1126/science.2432664 · DOI · Paper page
  18. Folkman, 1988, A heparin-binding angiogenic protein-basic fibroblast growth factor is stored within basement membrane, Amer. J. Pathol, 130, 393
  19. Franco, 1990, Calcium entry through stretch inactivated ion channels in mdx myotubes, Nature, 344, 670, 10.1038/344670a0 · DOI · Paper page
  20. Gajdusek, 1989, Injury-induced release of basic fibroblast growth factor from bovine aortic endothelium, J. Cell. Physiol, 139, 570, 10.1002/jcp.1041390317 · doi:10.1002/jcp.1041390317
  21. Gimlich, 1985, Improved fluorescent compounds for tracing cell lineage, Dev. Biol, 109, 509, 10.1016/0012-1606(85)90476-2 · DOI · Paper page
  22. Gonzalez, 1990, Distribution of basic fibroblast growth factor in the 18-day fetus: localization to the basement membrane of diverse tissues, J. Cell Biol, 110, 753, 10.1083/jcb.110.3.753 · DOI · Paper page
  23. Gospodarowicz, 1976, Effect of fibroblast growth factor on the division and fusion of bovine myoblasts, J. Cell Biol, 70, 395, 10.1083/jcb.70.2.395 · DOI
  24. Guthridge, 1992, The role of basic fibroblast growth factor in skeletal muscle regeneration, Growth Factors, 6, 53, 10.3109/08977199209008871 · DOI
  25. Hoffman, 1991, The animal models of Duchenne muscular dystrophy: windows on the pathophysiological consequences of dystrophin deficiency, Curr. Topics Membr, 38, 113154
  26. Hoffman, 1989, Dystrophin abnormalities in Duchenne/Becker muscular dystrophy, Neuron, 2, 1019, 10.1016/0896-6273(89)90226-2 · DOI · Paper page
  27. Hudecki, 1990, Mdx mouse as therapeutic model system: development and implementation of phenotypic monitoring, 251265, 10.1007/978-1-4684-5865-7_29 · DOI
  28. Hudecki, 1993, Strength and endurance in the therapeutic evaluation of prednisolone-treated mdx mouse, Res. Commun. Chem. Pathol. Pharm, 79, 45
  29. Ibraghimov-Beskrovnaya, 1992, Primary structure of dystrophin-associated glycoproteins linking dystrophin to the extracellular matrix, Nature, 355, 696, 10.1038/355696a0 · DOI · Paper page
  30. Johnson, 1981, A simple method of reducing the fading of immunofluorescence during microscopy, J. Immunol. Meth, 43, 349, 10.1016/0022-1759(81)90183-6 · DOI · Paper page
  31. Jones, 1984, Experimental mouse muscle damage: the importance of external calcium, Clin. Sci, 66, 317, 10.1042/cs0660317 · DOI
  32. Joseph-Silverstein, 1989, Basic fibroblast growth factor in the chick embryo: immunolocalization to striated muscle cells and their precursors, J. Cell Biol, 108, 2459, 10.1083/jcb.108.6.2459 · doi:10.1083/jcb.108.6.2459
  33. Kimmelman, 1988, The presence of fibroblast growth factor in the frog egg. Its role as a natural mesoderm inducer, Science, 242, 1053, 10.1126/science.3194757 · DOI · Paper page
  34. Klagsbrun, 1990, The affinity of fibroblast growth factors (FGFS) for heparin; FGF-heparan sulfate interactions in cells and extracellular matrix, Curr. Opin. Cell Biol, 2, 857, 10.1016/0955-0674(90)90084-R · DOI · Paper page
  35. Laemmli, 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680, 10.1038/227680a0 · DOI · Paper page
  36. Lemasters, 1987, Blebbing, free Ca2+ and mitochondrial membrane potential preceding cell death in hepatocytes, Nature, 325, 78, 10.1038/325078a0 · DOI · Paper page
  37. McNeil, 1988, Incorporation of macromolecules into living cells, Methods of Cell Biology, 29, 153, 10.1016/S0091-679X(08)60193-4 · DOI
  38. McNeil, 1989, Gastrointestinal cell membrane wounding and resealing in vivo, Gastroenterology, 96, 1238, 10.1016/S0016-5085(89)80010-1 · DOI
  39. McNeil, 1990, Molecular traffic through plasma membrane disruptions of cells in vivo, J. Cell Sci, 96, 549, 10.1242/jcs.96.3.549 · DOI
  40. McNeil, 1992, Disruptions of muscle fiber plasma membranes: role in exercise-induced damage, Amer. J. Path, 140, 10971109
  41. McNeil, 1989, Growth factors are released by mechanically wounded endothelial cells, J. Cell Biol, 109, 811, 10.1083/jcb.109.2.811 · DOI · Paper page
  42. Melan, 1991, Redistribution and differential extraction of soluble proteins in permeabilized cultured cells: implications for immunofluorescence microscopy, J. Cell Sci, 101, 731743
  43. Menke, 1991, Decreased osmotic stability of dystrophin-less muscle cells from the mdx mouse, Nature, 349, 69, 10.1038/349069a0 · DOI · Paper page
  44. Mignatti, 1991, Basic fibroblast growth factor released by single, isolated cells stimulates migration in an autocrine manner, Proc. Nat. Acad. Sci. USA, 88, 11007, 10.1073/pnas.88.24.11007 · DOI · Paper page
  45. Mignatti, 1992, Basic fibroblast growth factor, a protein devoid of secretory signal sequence, is released by cells via a pathway independent of the endoplasmic reticulum-Golgi complex, J. Cell. Physiol, 47, 201
  46. Morrow, 1990, Increased expression of fibroblast growth factors in a rabbit skeletal muscle model of exercise conditioning, J. Clin. Invest, 85, 1816, 10.1172/JCI114640 · DOI
  47. Muesch, 1990, A novel pathway for secretory proteins?, Trends Biochem. Sci, 15, 86, 10.1016/0968-0004(90)90186-F · DOI · Paper page
  48. Muthukrishnan, 1991, Basic fibroblast growth factor is efficiently released from a cytosolic storage site through plasma membrane disruptions of endothelial cells, J. Cell. Physiol, 148, 1, 10.1002/jcp.1041480102 · DOI · Paper page
  49. Nielsen, 1987, Association of calmodulin with lysosomes, J. Cell Sci, 87, 327, 10.1242/jcs.87.2.327 · DOI
  50. Petroff, 1993, Dystrophin protects the sarcolemma from stresses developed during muscle contraction, Proc. Nat. Acad. Sci. USA, 90, 3710, 10.1073/pnas.90.8.3710 · DOI · Paper page
  51. Renko, 1990, Nuclear and cytoplasmic localization of different basic fibroblast growth factor species, J. Cell. Physiol, 144, 108, 10.1002/jcp.1041440114 · DOI · Paper page
  52. Rifkin, 1989, Recent developments in the cell biology of basic fibroblast growth factor, J. Cell Biol, 109, 1, 10.1083/jcb.109.1.1 · DOI · Paper page
  53. Rapraeger, 1991, Requirement of heparan sulfate for bFGF-mediated fibroblast growth and myoblast differentiation, Science, 252, 1705, 10.1126/science.1646484 · DOI · Paper page
  54. Schultz, 1989, Satellite cell behavior during skeletal muscle growth and regeneration, Med. Sci. Sports Exercise, 21, S181, 10.1249/00005768-198910001-00010 · DOI · Paper page
  55. Schulze-Osthoff, 1990, In situ detection of basic fibroblast growth factor by highly specific antibodies, Amer. J. Pathol, 137, 85
  56. Seno, 1989, Monoclonal antibodies against human basic fibroblast growth factor, Hybridoma, 8, 209, 10.1089/hyb.1989.8.209 · doi:10.1089/hyb.1989.8.209
  57. Slack, 1987, Mesoderm induction in early Xenopus embryos by heparin-binding growth factors, Nature, 326, 197, 10.1038/326197a0 · DOI · Paper page
  58. Spirito, 1991, Immunohistochemical localization of basic and acidic fibroblast growth factors in the developing rat heart, Circulation, 84, 322, 10.1161/01.CIR.84.1.322 · doi:10.1161/01.cir.84.1.322
  59. Sunderkotter, 1990, Immunohistochemical detection of bFGF and TNF-b in the course of inflammatory angiogenesis in the mouse cornea, Amer. J. Pathol, 137, 511
  60. Tateishi, 1990, Immunohistochemical evidence of autocrine growth factors in adenocarcinoma of the human lung, Cancer Res, 50, 7077
  61. Weinberg, 1991, Amino acid protection of cultured kidney tubule cells against calcium ionophore-induced lethal cell injury, Lab. Invest, 65, 671
  62. Weller, 1990, Dystrophin-deficient mdx muscle fibers are preferentially vulnerable to necrosis induced by experimental lengthening contractions, J. Neurol.Sci, 100, 9, 10.1016/0022-510X(90)90005-8 · DOI · Paper page
  63. Weller, 1991, Glucocortocoids and immunosuppressants do not change the prevalence of necrosis and regeneration in mdx skeletal muscle, Muscle Nerve, 14, 771, 10.1002/mus.880140812 · DOI
  64. Vlodavsky, 1987, Endothelial cell-derived basic fibroblast growth factor: synthesis and deposition into subendothelial extracellular matrix, Proc. Nat. Acad. Sci. USA, 84, 2292, 10.1073/pnas.84.8.2292 · DOI · Paper page
  65. Yamada, 1989, Fibroblast growth factor is stored in fiber extracellular matrix and plays a role in regulating muscle hypertrophy, Med. Sci. Sports Exercise, 21, S173, 10.1249/00005768-198910001-00009 · DOI
  66. Yu, 1992, Transient disruptions of endothelial cells of the rat aorta, Amer. J. Pathol, 141, 1349
  67. Zagzag, 1990, Immunohistochemical localization of basic fibroblast growth factor in astrocytomas, Cancer Res, 50, 7393

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Metadata

Authors
Clarke, M. S. F., Khakee, R., & McNeil, P. L.
Venue
Journal of Cell Science
Publisher
The Company of Biologists
Published
1993-09-01
Volume
106
Issue
1
Pages
121-133
ISSN
  • 0021-9533 (ISSN-L)
  • 1477-9137
DOI
10.1242/jcs.106.1.121
OpenAlex
W2112510700
Cited by
206
References
2
FWCI
2.00
Type
journal article
Language
en
Open access
closed
Licence
www.biologists.com/user-licence-1-1/ (vor)

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CoChat. (2026). Citation check: Loss of cytoplasmic basic fibroblast growth factor from physiologically wounded myofibers of normal and dystrophic muscle. https://app.cochat.ai/papers/10.1242/jcs.106.1.121
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Clarke, M. S. F., Khakee, R., & McNeil, P. L. (1993). Loss of cytoplasmic basic fibroblast growth factor from physiologically wounded myofibers of normal and dystrophic muscle. Journal of Cell Science. https://doi.org/10.1242/jcs.106.1.121

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