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Intracellular processing of endocytosed triglyceride-rich lipoproteins comprises both recycling and degradation

Heeren, Jörg, Weber, Wilfried, Beisiegel, Ulrike·1999·Journal of Cell Science
DOI 10.1242/jcs.112.3.349
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Abstract

The current study was performed to investigate the intracellular fate of triglyceride-rich lipoproteins. Triglyceride-rich lipoproteins are responsible for the delivery of lipids to various tissues, however, their intracellular pathway has not yet been elucidated. Here radiolabeled triglyceride-rich lipoproteins, associated with lipoprotein lipase, were used for the quantitative evaluation of the intracellular metabolism. Pulse chase experiments showed that after 90 minutes approximately 60% of the labeled protein, mainly apoproteins E and C, was released intact into the medium, where it re-associates with lipoproteins. Apoprotein B, in contrast, was degraded, following the same pathway as the apoprotein B from low density lipoproteins. In kinetic experiments uptake and intracellular fate of triglyceride-rich lipoproteins was compared to that of transferrin and low density lipoproteins. These experiments revealed that apoproteins were retained inside the cell much longer than transferrin, and unlike low density lipoproteins were not degraded. Using immunofluorescence it was shown that apoprotein E and lipoprotein lipase follow a distinct route from the sorting compartment to the surface, which is clearly distinguishable from the perinuclear transferrin recycling compartment. In contrast, the fluorescence labeled lipids were delivered to lysosomal compartments. The data presented here show that surface proteins of triglyceride-rich lipoproteins, such as apoproteins E and C and lipoprotein lipase follow a recycling pathway, whereas lipids and high molecular mass core proteins are degraded.

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  • pH-dependent release of ApoE from its receptor in the early endosome is important for endosomal maturation, cargo delivery, and rapid recycling of endosomal content to the cell surface.

    “The pH-dependent release of ApoE from its receptor in the EE is important for endosomal maturation and cargo delivery (Yamamoto et al., 2008) for the ability of endosomal content to rapidly recycle to the cell surface (Heeren et al., 1999; Nixon, 2017).”

    From NHE6 depletion corrects ApoE4-mediated synaptic impairments and reduces amyloid plaque load (2021) · cites this paper as Heeren et al., 1999

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Metadata

Authors
Heeren, J., Weber, W., & Beisiegel, U.
Affiliations
Medical Clinic, University Hospital Eppendorf, Hamburg, Germany
Venue
Journal of Cell Science
Publisher
The Company of Biologists
Published
1999-02-01
Volume
112
Issue
3
Pages
349-359
ISSN
  • 0021-9533
  • 1477-9137
DOI
10.1242/jcs.112.3.349
Cited by
55
References
2
Language
en
Licence
www.biologists.com/user-licence-1-1/ (vor)

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