Cited by 45Antifungal resistance and susceptibilityFlavonoids in Medical ResearchFungal Plant Pathogen Control

Combination of Baicalein and Amphotericin B Accelerates Candida albicans Apoptosis

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Fu, ZiJin, Lu, Hui, Zhu, ZhenYu, Yan, Lan, Jiang, YuanYing, Cao, YingYing·2011·Biological and Pharmaceutical Bulletin

Abstract

Candida albicans is the most important human fungal pathogen. Amphotericin (AmB) is a gold standard of antifungal treatment for fungi, but the severe side effect of this drug restricts its clinical application. In this study, the interaction of AmB and baicalein (BE) was investigated against thirty clinical isolates of C. albicans. Synergistic activities were determined using the checkerboard microdilution assay based on the fractional inhibitory concentration indices. Combination of BE and AmB accelerated C. albicans apoptosis accompanied with an increase of reactive oxygen species (ROS). Moreover, AmB increased the caspase activity and expression of the corresponding gene CaMCA1 in C. albicans. These effects were enhanced in the presence of BE. Deletion of CaMCA1 clearly attenuated AmB-induced apoptosis, indicating the involvement of CaMCA1-mediated caspase pathway in AmB-induced apoptosis and the synergistic action.

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Crossref · 10.1248/bpb.34.214

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  • Baicalin, berberine, and osthole can elevate ROS levels in C. albicans, thereby bolstering antifungal potency of azoles.

    “Our previous investigations have demonstrated that baicalin, berberine, and osthole can elevate ROS levels in C. albicans, thereby bolstering the antifungal potency of azoles58–60.”

    From Impairing the interaction between Erg11 and cytochrome P450 reductase Ncp1 enhances azoles’ antifungal activities (2025) · cites this paper as [59]

    The opening summary reports increased ROS and antifungal synergy in C. albicans, but the tested combination was baicalein with amphotericin B—not baicalin with an azole. The Discussion mentions antifungal activity of baicalein with fluconazole, but does not link that combination to ROS; the cited paper does not establish the claim for berberine or osthole. (LLM, conf=0.87)

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Research works printed but not linked: 3

  1. Phillips A. J., Sudbery I., Ramsdale M., Proc. Natl. Acad. Sci. U.S.A., 100, 14327-14332 (2003).
  2. Phillips A. J., Crowe J. D., Ramsdale M., Proc. Natl. Acad. Sci. U.S.A., 103, 726-731 (2006).
  3. Khan M. A., Chock P. B., Stadtman E. R., Proc. Natl. Acad. Sci. U.S.A., 102, 17326-17331 (2005).

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Authors
Fu, Z., Lu, H., Zhu, Z., Yan, L., Jiang, Y., & Cao, Y.
Affiliations
Second Military Medical University (CN)
Venue
Biological and Pharmaceutical Bulletin
Published
2011
Volume
34
Issue
2
Pages
214-218
ISSN
  • 0918-6158 (ISSN-L)
  • 1347-5215
OpenAlex
W2016007336
Cited by
45
References
34
Citations by year
2025: 3 · 2024: 5 · 2023: 1 · 2022: 9 · 2021: 5 · 2020: 2 · 2019: 2 · 2018: 1 · 2017: 3 · 2016: 2 · 2015: 5 · 2014: 2 · 2013: 2 · 2012: 2
FWCI
1.51
Type
journal article
Language
en
Open access
Locations
  • Biological and Pharmaceutical Bulletin (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (30870105)
  • Changhai Hospital of Shanghai
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Health Sciences › Medicine › Pharmacology › Flavonoids in Medical Research
  • Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Fungal Plant Pathogen Control
Keywords
Candida albicans, baicalein, amphotericin B, drug synergy, apoptosis, reactive oxygen species, caspase activity, antifungal agents, combination therapy
MeSH terms
Amphotericin B, Antifungal Agents, Candida albicans, Drug Synergism, Plant Extracts, Gene Expression, Apoptosis, Reactive Oxygen Species, Caspases, Scutellaria baicalensis, Flavanones

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