Effects of Macelignan Isolated from Myristica fragrans (Nutmeg) on Expression of Matrix Metalloproteinase-1 and Type I Procollagen in UVB-Irradiated Human Skin Fibroblasts
Open accessAbstract
Exposure to ultraviolet (UV) light causes premature skin aging that is associated with upregulated matrix metalloproteinases (MMPs) and decreased collagen synthesis. Macelignan, a natural lignan compound isolated from Myristica fragrans HOUTT. (nutmeg), has been reported to possess antioxidant and antiinflammatory activities. This study assessed the effects of macelignan on photoaging and investigated its mechanisms of action in UV-irradiated human skin fibroblasts (Hs68) by reverse transcription-polymerase chain reaction, Western blot analysis, 2',7'-dichlorofluorescein diacetate assay, and enzyme-linked immunosorbent assay. Our results show that macelignan attenuated UV-induced MMP-1 expression by suppressing phosphorylation of mitogen-activated protein kinases (MAPKs) induced by reactive oxygen species. Macelignan also increased type I procollagen expression and secretion through transforming growth factor β (TGF-β)/Smad signaling. These findings indicate that macelignan regulates the expression of MMP-1 and type I procollagen in UV-irradiated human skin fibroblasts by modulating MAPK and TGF-β/Smad signaling, suggesting its potential as an efficacious antiphotoaging agent.
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Collagen is derived from HSF cells and plays an important role in maintaining the elasticity of skin.
“Collagen is derived from HSF cells and plays important role in maintaining the elasticity of skin (Lee et al., 2012).”
From A Frog Peptide Ameliorates Skin Photoaging Through Scavenging Reactive Oxygen Species (2022) · cites this paper as Lee et al., 2012
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Sorted by citations
A Frog Peptide Ameliorates Skin Photoaging Through Scavenging Reactive Oxygen Species
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- Authors
- Lee, K.-E., Mun, S., Pyun, H.-B., Kim, M.-S., & Hwang, J.-K.
- Affiliations
- Yonsei University (KR)
- Department of Biomaterial Science and Engineering, Yonsei University
- Department of Biotechnology, Yonsei University
- Venue
- Biological and Pharmaceutical Bulletin
- Publisher
- Pharmaceutical Society of Japan
- Published
- 2012
- Volume
- 35
- Issue
- 10
- Pages
- 1669-1675
- ISSN
- 0918-6158 (ISSN-L)
- 1347-5215
- OpenAlex
- W2068303185
- Cited by
- 33
- References
- 30
- Citations by year
- 2026: 1 · 2025: 7 · 2024: 2 · 2023: 1 · 2022: 2 · 2021: 1 · 2019: 4 · 2018: 3 · 2017: 3 · 2016: 3 · 2015: 1 · 2014: 4 · 2013: 1
- FWCI
- 2.40
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biological and Pharmaceutical Bulletin (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Yonsei University
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioactive natural compounds
- Keywords
- macelignan, nutmeg, photoaging, UVB irradiation, MMP-1, type I procollagen, dermal fibroblasts, MAPK signaling, TGF-β/Smad signaling, reactive oxygen species, antioxidant activity, anti-inflammatory activity, collagen synthesis
- MeSH terms
- Cell Line, Cell Survival, Fibroblasts, Humans, RNA, Messenger, Skin, Ultraviolet Rays, Lignans, Protective Agents, Matrix Metalloproteinase 1, Mitogen-Activated Protein Kinases, Collagen Type I, Myristica
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