The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Deadenylation is a widespread effect of miRNA regulation
Open accessAbstract
miRNAs silence gene expression by repressing translation and/or by promoting mRNA decay. In animal cells, degradation of partially complementary miRNA targets occurs via deadenylation by the CAF1-CCR4-NOT1 deadenylase complex, followed by decapping and subsequent exonucleolytic digestion. To determine how generally miRNAs trigger deadenylation, we compared mRNA expression profiles in D. melanogaster cells depleted of AGO1, CAF1, or NOT1. We show that ∼60% of AGO1 targets are regulated by CAF1 and/or NOT1, indicating that deadenylation is a widespread effect of miRNA regulation. However, neither a poly(A) tail nor mRNA circularization are required for silencing, because mRNAs whose 3′ ends are generated by a self-cleaving ribozyme are also silenced in vivo. We show further that miRNAs trigger mRNA degradation, even when binding by 40S ribosomal subunits is inhibited in cis. These results indicate that miRNAs promote mRNA decay by altering mRNP composition and/or conformation, rather than by directly interfering with the binding and function of ribosomal subunits.
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Cites
The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14
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Silencing of microRNAs in vivo with ‘antagomirs’
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The impact of microRNAs on protein output
Baek, D., Villén, J., Shin, C., Camargo, F. D., Gygi, S. P., & Bartel, D. P. (2008) · Nature · doi:10.1038/nature07242 · Cited by 3,592
Widespread changes in protein synthesis induced by microRNAs
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Zebrafish MiR-430 Promotes Deadenylation and Clearance of Maternal mRNAs
Giraldez, A. J., Mishima, Y., Rihel, J., Grocock, R. J., Van Dongen, S., Inoue, K., Enright, A. J., & Schier, A. F. (2006) · Science · doi:10.1126/science.1122689 · Cited by 1,647
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Cited by
Mammalian microRNAs predominantly act to decrease target mRNA levels
Guo, H., Ingolia, N. T., Weissman, J. S., & Bartel, D. P. (2010) · Nature · doi:10.1038/nature09267 · Cited by 3,886
Regulation of mRNA Translation and Stability by microRNAs
Fabian, M. R., Sonenberg, N., & Filipowicz, W. (2010) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060308-103103 · Cited by 3,214
Gene silencing by microRNAs: contributions of translational repression and mRNA decay
Huntzinger, E., & Izaurralde, E. (2011) · Nature Reviews Genetics · doi:10.1038/nrg2936 · Cited by 2,339
Self-citationTowards a molecular understanding of microRNA-mediated gene silencing
Jonas, S., & Izaurralde, E. (2015) · Nature Reviews Genetics · doi:10.1038/nrg3965 · Cited by 1,872
miRNA-mediated gene silencing by translational repression followed by mRNA deadenylation and decay
Djuranovic, S., Nahvi, A., & Green, R. (2012) · Science · doi:10.1126/science.1215691 · Cited by 811
Metadata
- Authors
- Eulalio, A., Huntzinger, E., Nishihara, T., Rehwinkel, J., Fauser, M., & Izaurralde, E.
- Venue
- RNA
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2008-11-24
- Volume
- 15
- Issue
- 1
- Pages
- 21-32
- ISSN
- 1355-8382 (ISSN-L)
- 1469-9001
- OpenAlex
- W2147114301
- Cited by
- 430
- References
- 7
- FWCI
- 11.91
- Type
- journal article
- Language
- en
- Open access
- hybrid
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