Effects of Isoflavone and Exercise on BMD and Fat Mass in Postmenopausal Japanese Women: A 1-Year Randomized Placebo-Controlled Trial
Open accessAbstract
The combined intervention of isoflavone intake and walking exercise over 1 year in postmenopausal Japanese women exhibited a trend for a greater effect on prevention of bone loss at the total hip and Ward's triangle regions. Introduction: The additive effects of isoflavones and exercise on bone and lipid metabolism have been shown in estrogen-deficient animals. In this study, we determined the effects of isoflavone intake, walking exercise, and their interaction on bone, fat mass, and lipid metabolism over 1 year in postmenopausal Japanese women. Materials and Methods: A total of 136 postmenopausal women at <5 years after the onset of menopause were randomly assigned to four groups: (1) placebo, (2) walking (45 minutes/day, 3 days/week) with placebo, (3) isoflavone intake (75 mg of isoflavone conjugates/day), and (4) combination of isoflavone plus walking. BMD, fat mass, serum lipid, and serum and urinary isoflavone concentrations were assessed. Results: A significant main effect of isoflavone on the reduction in trunk fat mass was obtained at 12 months. Significant main effects of walking on the reduction in fat mass in the whole body and the trunk were observed at 3, 6, and 12 months and that in the legs and arms at 6 and 12 months. Serum high-density lipoprotein (HDL)-cholesterol concentration significantly increased by 12 months after the walking and the combined intervention. After 12 months, a significant main effect of isoflavone on BMD was observed only at Ward's triangle. Walking prevented bone loss at the total hip and the Ward's triangle to significant degrees. The effect of the combined intervention on BMD at total hip and Ward's triangle regions was greater than that of either alone. No significant interaction was observed between isoflavone and walking in any measurements recorded during the study. Conclusions: Our study suggest that combined intervention of 75 mg/day of isoflavone intake and walking exercise 3 times/week for 1 year showed a trend for a greater effect on BMD at total hip and Ward's triangle regions than either alone. Intervention with isoflavone in postmenopausal Japanese women showed a modest effect on BMD compared with those in Westerners. Further studies over longer treatment duration that include assessment of BMD at various regions are necessary to ascertain the clinical significance of the combined intervention of isoflavone plus walking in postmenopausal women.
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Genistein reverses truncal fat accumulation in postmenopausal women and ovariectomized rodent models.
“However, Genistein reverses the truncal fat accumulation in postmenopausal women and ovariectomized rodent models [144, 145].”
From Estrogen deficiency and the origin of obesity during menopause (2014) · cites this paper as [144]
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Cites
Writing Group for the Women's Health Initiative Investigators (2002) · JAMA: The Journal of the American Medical Association · doi:10.1001/jama.288.3.321 · Cited by 15,889 · see citing works
Bone density at various sites for prediction of hip fractures
Cummings, S. R., Browner, W., Cummings, S. R., Black, D. M., Nevitt, M. C., Browner, W., Genant, H. K., Cauley, J., Ensrud, K., Scott, J., et al. (1993) · The Lancet · doi:10.1016/0140-6736(93)92555-8 · Cited by 2,446 · see citing works
Gebbie, A. E. (2002) · Journal of Family Planning and Reproductive Health Care · doi:10.1783/147118902101196685 · Cited by 1,178 · see citing works
Three rules for bone adaptation to mechanical stimuli
Turner, C. H. (1998) · Bone · doi:10.1016/s8756-3282(98)00118-5 · Cited by 971 · see citing works
Wolff, I., van Croonenborg, J. J., Kemper, H. C. G., Kostense, P. J., & Twisk, J. W. R. (1999) · Osteoporosis International · doi:10.1007/s001980050109 · Cited by 589 · see citing works
Anderson, G. L. (2003) · JAMA · doi:10.1001/jama.290.13.1739 · Cited by 541 · see citing works
Axial and appendicular bone density predict fractures in older women
Black, D. M., Cummings, S. R., Genant, H. K., Nevitt, M. C., Palermo, L., & Browner, W. S. (1992) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.5650070607 · Cited by 509 · see citing works
Effect of menopausal status on body composition and abdominal fat distribution
Toth, M., Tchernof, A., Sites, C., & Poehlman, É. T. (2000) · International Journal of Obesity · doi:10.1038/sj.ijo.0801118 · Cited by 504 · see citing works
Kreijkamp-Kaspers, S., Kok, L., Grobbee, D. E., de Haan, E. H. F., Aleman, A., Lampe, J. W., & van der Schouw, Y. T. (2004) · JAMA · doi:10.1001/jama.292.1.65 · Cited by 438 · see citing works
Morabito, N., Crisafulli, A., Vergara, C., Gaudio, A., Lasco, A., Frisina, N., D’Anna, R., Corrado, F., Pizzoleo, M. A., Cincotta, M., et al. (2002) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.2002.17.10.1904 · Cited by 432 · see citing works
Setchell, K. D. R., & Lydeking-Olsen, E. (2003) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/78.3.593s · Cited by 369 · see citing works
Ensrud, K. E., Palermo, L., Black, D. M., Cauley, J. A., Jergas, M. D., ORWOLL, E. S., Nevitt, M. C., Fox, K. M., & Cummings, S. R. (1995) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.5650101122 · Cited by 357 · see citing works
Additive effects of weight-bearing exercise and estrogen on bone mineral density in older women
Kohrt, W. M., Snead, D. B., Slatopolsky, E., & Birge, S. J. (1995) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.5650100906 · Cited by 306 · see citing works
ARLOT, M. E., Sornay‐Rendu, E., Garnero, P., Vey-Marty, B., & Delmas, P. D. (1997) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.1997.12.4.683 · Cited by 300 · see citing works
Atkinson, C., Compston, J. E., Day, N., Dowsett, M., & Bingham, S. A. (2004) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/79.2.326 · Cited by 282 · see citing works
Dietary phyto-oestrogens and the menopause in Japan
Adlercreutz, H., Hämäläinen, E. K., Gorbach, S. L., & Goldin, B. R. (1992) · The Lancet · doi:10.1016/0140-6736(92)91174-7 · Cited by 277 · see citing works
Ishimi, Y., Miyaura, C., Ohmura, M., et al. (1999) · Endocrinology · doi:10.1210/endo.140.4.6663 · Cited by 254 · see citing works
Self-citationChen, Y., Ho, S. C., Ho, S. C., et al. (2003) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2003-030290 · Cited by 205 · see citing works
Difference in Effective Dosage of Genistein on Bone and Uterus in Ovariectomized Mice
Ishimi, Y., Arai, N., Wang, X., Wu, J., Umegaki, K., Miyaura, C., Takeda, A., & Ikegami, S. (2000) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2000.3175 · Cited by 175 · see citing works
Self-citationRelationship between Accumulated Walking and Body Composition in Middle-Aged Women
Thompson, D. L., Rakow, J., & Perdue, S. (2004) · Medicine & Science in Sports & Exercise · doi:10.1249/01.mss.0000126787.14165.b3 · Cited by 162 · see citing works
Dewell, A., Hollenbeck, C. B., & Bruce, B. (2002) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.87.1.8155 · Cited by 134 · see citing works
Uesugi, T., Toda, T., Tsuji, K., et al. (2001) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.24.368 · Cited by 127 · see citing works
Self-citationExercise Training Improves Fat Distribution Patterns in 60-to 70-year-old Men and Women
Kohrt, W. M., Obert, K. A., & Holloszy, J. O. (1992) · Journal of Gerontology · doi:10.1093/geronj/47.4.m99 · Cited by 126 · see citing works
Samaras, K., Hayward, C., SULLIVAN, D. R., Kelly, R. P., & Campbell, L. V. (1999) · Diabetes Care · doi:10.2337/diacare.22.9.1401 · Cited by 120 · see citing works
Jensen, L., Vestergaard, P., Hermann, A., Gram, J., Eiken, P., Abrahamsen, B., Brot, C., Kolthoff, N., Sørensen, O., Beck-Nielsen, H., et al. (2003) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.2003.18.2.333 · Cited by 119 · see citing works
Wu, J., Wang, X., Chiba, H., Higuchi, M., Nakatani, T., Ezaki, O., Cui, H., Yamada, K., & Ishimi, Y. (2004) · Metabolism · doi:10.1016/j.metabol.2004.01.019 · Cited by 118 · see citing works
Self-citationCheng, S., Sipilä, S., Taaffe, D. R., Puolakka, J. O., & Suominen, H. (2002) · Bone · doi:10.1016/s8756-3282(02)00794-9 · Cited by 117 · see citing works
Ravn, P., Hetland, M. L., Overgaard, K., et al. (1994) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.5650091218 · Cited by 110 · see citing works
Kohrt, W. M., Ehsani, A. A., & Birge, S. J. (1998) · Journal of Applied Physiology · doi:10.1152/jappl.1998.84.5.1506 · Cited by 108 · see citing works
Soy Isoflavones and Bone Health: The Relationship Is Still Unclear
Weaver, C. M., & Cheong, J. M. K. (2005) · Journal of Nutrition · doi:10.1093/jn/135.5.1243 · Cited by 95 · see citing works
Resistance Training in Postmenopausal Women with and without Hormone Therapy
Teixeira, P. J., Going, S. B., HOUTKOOPER, L. B., METCALFE, L. L., Blew, R. M., FLINT-WAGNER, H. G., CUSSLER, E. C., Sardinha, L. B., & Lohman, T. G. (2003) · Medicine & Science in Sports & Exercise · doi:10.1249/01.mss.0000058437.17262.11 · Cited by 92 · see citing works
Wu, J., Wang, X. X., Takasaki, M., et al. (2001) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.2001.16.10.1829 · Cited by 90 · see citing works
Self-citationGuyatt, G. H., Cranney, A., Griffith, L. E., Walter, S. D., Krolicki, N., Favus, M. J., & Rosen, C. J. (2002) · Endocrinology and Metabolism Clinics of North America · doi:10.1016/s0889-8529(02)00024-5 · Cited by 85 · see citing works
Ishimi, Y., Yoshida, M., Wakimoto, S., Wu, J., Chiba, H., Wang, X., Takeda, K., & Miyaura, C. (2002) · Bone · doi:10.1016/s8756-3282(02)00780-9 · Cited by 77 · see citing works
Self-citationEvans, E. M., Pelt, R. E. V., Binder, E. F., Williams, D. B., Ehsani, A. A., & Kohrt, W. M. (2001) · Journal of Applied Physiology · doi:10.1152/jappl.2001.90.6.2033 · Cited by 74 · see citing works
Lipoprotein abnormalities related to women’s health
Bittner, V. (2002) · The American Journal of Cardiology · doi:10.1016/s0002-9149(02)02637-1 · Cited by 72 · see citing works
Uesugi, S., Watanabe, S., Ishiwata, N., et al. (2004) · BioFactors · doi:10.1002/biof.5520220145 · Cited by 64 · see citing works
Suppressive Effects of Genistein Dosage and Resistance Exercise on Bone Loss in Ovariectomized Rats.
Nakajima, D., Kim, C.-S., Oh, T.-W., Yang, C.-Y., Naka, T., Igawa, S., & Fukio, O. H. T. A. (2001) · Journal of PHYSIOLOGICAL ANTHROPOLOGY and Applied Human Science · doi:10.2114/jpa.20.285 · Cited by 58 · see citing works
Wu, J., Wang, X. X., Chiba, H., Higuchi, M., Takasaki, M., Ohta, A., & Ishimi, Y. (2003) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00498.2002 · Cited by 43 · see citing works
Self-citationSanada, M., Higashi, Y., Nakagawa, K., Tsuda, M., Kodama, I., Kimura, M., Chayama, K., & Ohama, K. (2002) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2001-011223 · Cited by 33 · see citing works
Turner, C. H. (1998) · Bone · doi:10.1016/s8756-3282(98)00088-x · Cited by 27 · see citing works
Vigna, G. B., Donegagrave, P., Zanca, R., Barban, A., Passaro, A., Pansini, F. S., Bonaccorsi, G., Mollica, G., & Fellin, R. (2002) · Metabolism · doi:10.1053/meta.2002.35584 · Cited by 25 · see citing works
Darling, G. M., Johns, J., McCloud, P., & Davis, S. R. (1999) · Climacteric · doi:10.3109/13697139909038060 · Cited by 21 · see citing works
Long-term effects of hormone replacement therapy
Stevenson, J. C. (2003) · The Lancet · doi:10.1016/s0140-6736(03)12279-9 · Cited by 16 · see citing works
Chilibeck, P. D. (2004) · Canadian Journal of Applied Physiology · doi:10.1139/h04-006 · Cited by 12 · see citing works
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Kerksick, C. M., Wilborn, C. D., Roberts, M. D., Smith-Ryan, A., Kleiner, S. M., Jäger, R., Collins, R., Cooke, M., Davis, J. N., Galvan, E., et al. (2018) · Journal of the International Society of Sports Nutrition · doi:10.1186/s12970-018-0242-y · Cited by 945 · see citing works
Estrogen deficiency and the origin of obesity during menopause
Lizcano, F., & Guzmán, G. (2014) · BioMed Research International · doi:10.1155/2014/757461 · Cited by 586 · see citing works
Minireview: The Case for Obesogens
Grün, F., & Blumberg, B. (2009) · Molecular Endocrinology · doi:10.1210/me.2008-0485 · Cited by 188 · see citing works
Kemmler, W., Shojaa, M., Kohl, M., & von Stengel, S. (2020) · Calcified Tissue International · doi:10.1007/s00223-020-00744-w · Cited by 161 · see citing works
Poggiogalle, E., Migliaccio, S., Lenzi, A., & Donini, L. M. (2014) · Endocrine · doi:10.1007/s12020-014-0315-x · Cited by 67 · see citing works
- Authors
- Wu, J., Oka, J., Tabata, I., Higuchi, M., Toda, T., Fuku, N., Ezaki, J., Sugiyama, F., Uchiyama, S., Yamada, K., & Ishimi, Y.
- Affiliations
- National Institute of Health and Nutrition (JP)
- Fujicco Co., Ltd. (Japan) (JP)
- Otsuka (Japan) (JP)
- Corresponding author
- Ishimi, Yoshiko
- Venue
- Journal of Bone and Mineral Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2006-05-01
- Volume
- 21
- Issue
- 5
- Pages
- 780-789
- ISSN
- 0884-0431 (ISSN-L)
- 1523-4681
- OpenAlex
- W2053602264
- Cited by
- 140
- References
- 45
- Citations by year
- 2026: 1 · 2025: 8 · 2024: 1 · 2023: 5 · 2022: 7 · 2021: 4 · 2020: 14 · 2019: 3 · 2018: 4 · 2017: 4 · 2016: 9 · 2015: 6 · 2014: 9 · 2013: 8 · 2012: 14
- FWCI
- 5.13
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Journal of Bone and Mineral Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Tokyo University of Agriculture
- Japan Society for the Promotion of Science (16500536)
- Topics
- Health Sciences › Medicine › Pathology and Forensic Medicine › Phytoestrogen effects and research
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Menopause: Health Impacts and Treatments
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Keywords
- isoflavones, walking exercise, bone mineral density, postmenopausal women, fat mass, bone loss prevention, Ward's triangle, total hip, HDL cholesterol, serum lipids, estrogen deficiency, combined intervention, Japanese women, lipid metabolism, trunk fat, urinary isoflavone excretion
- MeSH terms
- Estradiol, Female, Humans, Isoflavones, Lipids, Middle Aged, Placebos, Surveys and Questionnaires, Biomarkers, Exercise, Bone Density, Postmenopause, Adiposity
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