The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy
Open accessAbstract
We have developed a digital holographic microscope (DHM), in a transmission mode, adapted to the quantitative study of cellular dynamics. Living cells in culture are optically probed by measuring the phase shift they produce on the transmitted wave front. The high temporal stability of the phase signal, equivalent to lambda/1800, and the low acquisition time (~20micros) enable to monitor cellular dynamics processes. An experimental procedure allowing to calculate both the integral refractive index and the cellular thickness (morphometry) from the measured phase shift is presented. Specifically, the method has been applied to study the dynamics of neurons in culture during a hypotonic stress. Such stress produces a paradoxical decrease of the phase which can be entirely resolved by applying the methodological approach described in this article; indeed the method allows to determine independently the thickness and the integral refractive index of cells.
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Cites
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Self-citationRefractive index of some mammalian tissues using a fiber optic cladding method
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Cited by
Choi, W., Fang-Yen, C., Badizadegan, K., Oh, S., Lue, N., Dasari, R. R., & Feld, M. S. (2007) · Nature Methods · doi:10.1038/nmeth1078 · Cited by 955
Diffraction phase microscopy: principles and applications in materials and life sciences
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L-Lactate protects neurons against excitotoxicity: implication of an ATP-mediated signaling cascade
Jourdain, P., Allaman, I., Rothenfusser, K., Fiumelli, H., Marquet, P., & Magistretti, P. J. (2016) · Scientific Reports · doi:10.1038/srep21250 · Cited by 116
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Metadata
- Authors
- Rappaz, B., Marquet, P., Cuche, E., Emery, Y., Depeursinge, C., & Magistretti, P. J.
- Venue
- Optics Express
- Publisher
- Optica Publishing Group
- Published
- 2005-11-14
- Volume
- 13
- Issue
- 23
- Pages
- 9361
- ISSN
- 1094-4087 (ISSN-L)
- OpenAlex
- W2014002533
- Cited by
- 674
- References
- 15
- FWCI
- 11.48
- Type
- journal article
- Language
- en
- Open access
- gold
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- Rappaz, B., Marquet, P., Cuche, E., Emery, Y., Depeursinge, C., & Magistretti, P. J. (2005). Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy. Optics Express. https://doi.org/10.1364/opex.13.009361
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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