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Natural and Experimental Infection of Caenorhabditis Nematodes by Novel Viruses Related to Nodaviruses
Open accessAbstract
An ideal model system to study antiviral immunity and host-pathogen co-evolution would combine a genetically tractable small animal with a virus capable of naturally infecting the host organism. The use of C. elegans as a model to define host-viral interactions has been limited by the lack of viruses known to infect nematodes. From wild isolates of C. elegans and C. briggsae with unusual morphological phenotypes in intestinal cells, we identified two novel RNA viruses distantly related to known nodaviruses, one infecting specifically C. elegans (Orsay virus), the other C. briggsae (Santeuil virus). Bleaching of embryos cured infected cultures demonstrating that the viruses are neither stably integrated in the host genome nor transmitted vertically. 0.2 µm filtrates of the infected cultures could infect cured animals. Infected animals continuously maintained viral infection for 6 mo (∼50 generations), demonstrating that natural cycles of horizontal virus transmission were faithfully recapitulated in laboratory culture. In addition to infecting the natural C. elegans isolate, Orsay virus readily infected laboratory C. elegans mutants defective in RNAi and yielded higher levels of viral RNA and infection symptoms as compared to infection of the corresponding wild-type N2 strain. These results demonstrated a clear role for RNAi in the defense against this virus. Furthermore, different wild C. elegans isolates displayed differential susceptibility to infection by Orsay virus, thereby affording genetic approaches to defining antiviral loci. This discovery establishes a bona fide viral infection system to explore the natural ecology of nematodes, host-pathogen co-evolution, the evolution of small RNA responses, and innate antiviral mechanisms.
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1 retracted
RETRACTED: Secondary siRNAs Result from Unprimed RNA Synthesis and Form a Distinct Class
Sijen, T., Steiner, F. A., Thijssen, K. L., & Plasterk, R. H. A. (2007) · Science · doi:10.1126/science.1136699 · Cited by 383
RetractedPotent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998) · Nature · doi:10.1038/35888 · Cited by 15,429
The Human Genome Browser at UCSC
Kent, W. J., Sugnet, C. W., Furey, T. S., Roskin, K. M., Pringle, T. H., Zahler, A. M., & Haussler, A. D. (2002) · Genome Research · doi:10.1101/gr.229102 · Cited by 10,400
Systematic functional analysis of the Caenorhabditis elegans genome using RNAi
Kamath, R. S., Fraser, A. G., Dong, Y., Poulin, G., Durbin, R., Gotta, M., Kanapin, A., Le Bot, N., Moreno, S., Sohrmann, M., et al. (2003) · Nature · doi:10.1038/nature01278 · Cited by 3,616
The Nematode Caenorhabditis elegans
Wood, W. B. (1988) · Cold Spring Harbor Laboratory eBooks · Cited by 2,820
Tetherin inhibits retrovirus release and is antagonized by HIV-1 Vpu
Neil, S. J. D., Zang, T., & Bieniasz, P. D. (2008) · Nature · doi:10.1038/nature06553 · Cited by 1,902
The rde-1 Gene, RNA Interference, and Transposon Silencing in C. elegans
Tabara, H., Sarkissian, M., Kelly, W. G., Fleenor, J., Grishok, A., Timmons, L., Fire, A., & Mello, C. C. (1999) · Cell · doi:10.1016/s0092-8674(00)81644-x · Cited by 1,282
Induction and Suppression of RNA Silencing by an Animal Virus
Li, H., Li, W. X., & Ding, S. W. (2002) · Science · doi:10.1126/science.1070948 · Cited by 868
A Conserved p38 MAP Kinase Pathway in Caenorhabditis elegans Innate Immunity
Kim, D. H., Feinbaum, R., Alloing, G., Emerson, F. E., Garsin, D. A., Inoue, H., Tanaka-Hino, M., Hisamoto, N., Matsumoto, K., Tan, M.-W., et al. (2002) · Science · doi:10.1126/science.1073759 · Cited by 853
Distinct Populations of Primary and Secondary Effectors During RNAi in C. elegans
Pak, J., & Fire, A. (2007) · Science · doi:10.1126/science.1132839 · Cited by 599
Distinct Argonaute-Mediated 22G-RNA Pathways Direct Genome Surveillance in the C. elegans Germline
Gu, W., Shirayama, M., Conte, D., Vasale, J., Batista, P. J., Claycomb, J. M., Moresco, J. J., Youngman, E. M., Keys, J., Stoltz, M. J., et al. (2009) · Molecular Cell · doi:10.1016/j.molcel.2009.09.020 · Cited by 505
Viral Discovery and Sequence Recovery Using DNA Microarrays
Wang, D., Urisman, A., Liu, Y.-T., Springer, M., Ksiazek, T. G., Erdman, D. D., Mardis, E. R., Hickenbotham, M., Magrini, V., Eldred, J., et al. (2003) · PLoS Biology · doi:10.1371/journal.pbio.0000002 · Cited by 465
Self-citationDrosophila RNAi screen identifies host genes important for influenza virus replication
Hao, L., Sakurai, A., Watanabe, T., Sorensen, E., Nidom, C. A., Newton, M. A., Ahlquist, P., & Kawaoka, Y. (2008) · Nature · doi:10.1038/nature07151 · Cited by 425
Animal virus replication and RNAi-mediated antiviral silencing in Caenorhabditis elegans
Lu, R., Maduro, M., Li, F., Li, H. W., Broitman-Maduro, G., Li, W. X., & Ding, S. W. (2005) · Nature · doi:10.1038/nature03870 · Cited by 422
Das, P. P., Bagijn, M. P., Goldstein, L. D., Woolford, J. R., Lehrbach, N. J., Sapetschnig, A., Buhecha, H. R., Gilchrist, M. J., Howe, K. L., Stark, R., et al. (2008) · Molecular Cell · doi:10.1016/j.molcel.2008.06.003 · Cited by 418
Self-citationRNA interference is an antiviral defence mechanism in Caenorhabditis elegans
Wilkins, C., Dishongh, R., Moore, S. C., Whitt, M. A., Chow, M., & Machaca, K. (2005) · Nature · doi:10.1038/nature03957 · Cited by 354
Microsporidia Are Natural Intracellular Parasites of the Nematode Caenorhabditis elegans
Troemel, E. R., Félix, M.-A., Whiteman, N. K., Barrière, A., & Ausubel, F. M. (2008) · PLoS Biology · doi:10.1371/journal.pbio.0060309 · Cited by 262
Self-citationSchulte, R. D., Makus, C., Hasert, B., Michiels, N. K., & Schulenburg, H. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1003113107 · Cited by 232
LIN-28 and the poly(U) polymerase PUP-2 regulate let-7 microRNA processing in Caenorhabditis elegans
Lehrbach, N. J., Armisen, J., Lightfoot, H. L., Murfitt, K. J., Bugaut, A., Balasubramanian, S., & Miska, E. A. (2009) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.1675 · Cited by 229
Self-citationSabin, L. R., Zhou, R., Gruber, J. J., Lukinova, N., Bambina, S., Berman, A., Lau, C.-K., Thompson, C. B., & Cherry, S. (2009) · Cell · doi:10.1016/j.cell.2009.04.045 · Cited by 215
RNA‐based viral immunity initiated by the Dicer family of host immune receptors
Aliyari, R., & Ding, S. (2009) · Immunological Reviews · doi:10.1111/j.1600-065x.2008.00722.x · Cited by 199
An antiviral role for the RNA interference machinery in Caenorhabditis elegans
Schott, D. H., Cureton, D. K., Whelan, S. P., & Hunter, C. P. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0507123102 · Cited by 183
Tijsterman, M., Okihara, K. L., Thijssen, K., & Plasterk, R. H. A. (2002) · Current Biology · doi:10.1016/s0960-9822(02)01110-7 · Cited by 173
Chapter 17 Electron Microscopy and Three-Dimensional Image Reconstruction
Hall, D. H. (1995) · Methods in Cell Biology · doi:10.1016/s0091-679x(08)61397-7 · Cited by 166
Powell, J. R., Kim, D. H., & Ausubel, F. M. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0813048106 · Cited by 131
Lu, R., Yigit, E., Li, W.-X., & Ding, S. (2009) · PLoS Pathogens · doi:10.1371/journal.ppat.1000286 · Cited by 130
Cryptic Quantitative Evolution of the Vulva Intercellular Signaling Network in Caenorhabditis
Félix, M. (2007) · Current Biology · doi:10.1016/j.cub.2006.12.024 · Cited by 110
Self-citationIsolation of C. elegans and related nematodes
Institut de Biologie du Développement de Marseille-Luminy, C., & Barrière, A. (2014) · WormBook · doi:10.1895/wormbook.1.115.2 · Cited by 105
Milloz, J., Duveau, F., Nuez, I., & Félix, M. (2008) · Genes & Development · doi:10.1101/gad.495308 · Cited by 97
Self-citationWeimer, R. M. (2006) · Humana Press eBooks · doi:10.1385/1-59745-151-7:203 · Cited by 91
Chapter 6 Drosophila Viruses and the Study of Antiviral Host‐Defense
Huszar, T. I., & Imler, J. (2008) · Advances in virus research · doi:10.1016/s0065-3527(08)00406-5 · Cited by 84
Spontaneous Mutational Variation for Body Size inCaenorhabditis elegans
Azevedo, R. B. R., Keightley, P. D., Laurén‐Määttä, C., Vassilieva, L. L., Lynch, M., & Leroi, A. M. (2002) · Genetics · doi:10.1093/genetics/162.2.755 · Cited by 70
Hüsken, K., Wiesenfahrt, T., Abraham, C., Windoffer, R., Bossinger, O., & Leube, R. E. (2008) · Differentiation · doi:10.1111/j.1432-0436.2008.00264.x · Cited by 44
Liu, W., Lin, Y., Wang, J.-P., Liou, W., Hou, R. F., Wu, Y., & Liao, C. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0506442103 · Cited by 40
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Early life dynamics of the human gut virome and bacterial microbiome in infants
Lim, E. S., Zhou, Y., Zhao, G., Bauer, I. K., Droit, L., Ndao, I. M., Warner, B. B., Tarr, P. I., Wang, D., & Holtz, L. R. (2015) · Nature Medicine · doi:10.1038/nm.3950 · Cited by 658
Self-citationInsulin/insulin-like growth factor signaling in C. elegans
Murphy, C. T. (2013) · WormBook · doi:10.1895/wormbook.1.164.1 · Cited by 532
Small RNA-based antimicrobial immunity
Guo, Z., Li, Y., & Ding, S.-W. (2019) · Nature Reviews Immunology · doi:10.1038/s41577-018-0071-x · Cited by 391
Transgenerational Inheritance of an Acquired Small RNA-Based Antiviral Response in C. elegans
Rechavi, O., Minevich, G., & Hobert, O. (2011) · Cell · doi:10.1016/j.cell.2011.10.042 · Cited by 351
Sequence Analysis of the Human Virome in Febrile and Afebrile Children
Wylie, K. M., Mihindukulasuriya, K. A., Sodergren, E., Weinstock, G. M., & Storch, G. A. (2012) · PLoS ONE · doi:10.1371/journal.pone.0027735 · Cited by 193
Metadata
- Authors
- Félix, M.-A., Ashe, A., Piffaretti, J., Wu, G., Nuez, I., Bélicard, T., Jiang, Y., Zhao, G., Franz, C. J., Goldstein, L. D., Sanroman, M., Miska, E. A., & Wang, D.
- Venue
- PLoS Biology
- Publisher
- Public Library of Science (PLoS)
- Published
- 2011-01-25
- Volume
- 9
- Issue
- 1
- Pages
- e1000586
- ISSN
- 1544-9173 (ISSN-L)
- 1545-7885
- OpenAlex
- W2020639485
- Cited by
- 416
- References
- 34
- FWCI
- 12.76
- Type
- journal article
- Language
- en
- Open access
- gold
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