The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Bio-On-Magnetic-Beads (BOMB): Open platform for high-throughput nucleic acid extraction and manipulation
Open accessAbstract
Current molecular biology laboratories rely heavily on the purification and manipulation of nucleic acids. Yet, commonly used centrifuge- and column-based protocols require specialised equipment, often use toxic reagents, and are not economically scalable or practical to use in a high-throughput manner. Although it has been known for some time that magnetic beads can provide an elegant answer to these issues, the development of open-source protocols based on beads has been limited. In this article, we provide step-by-step instructions for an easy synthesis of functionalised magnetic beads, and detailed protocols for their use in the high-throughput purification of plasmids, genomic DNA, RNA and total nucleic acid (TNA) from a range of bacterial, animal, plant, environmental and synthetic sources. We also provide a bead-based protocol for bisulfite conversion and size selection of DNA and RNA fragments. Comparison to other methods highlights the capability, versatility, and extreme cost-effectiveness of using magnetic beads. These open-source protocols and the associated webpage (https://bomb.bio) can serve as a platform for further protocol customisation and community engagement.
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Metadata
- Authors
- Oberacker, P., Stepper, P., Bond, D. M., Höhn, S., Focken, J., Meyer, V., Schelle, L., Sugrue, V. J., Jeunen, G.-J., Moser, T., Hore, S. R., von Meyenn, F., Hipp, K., Hore, T. A., & Jurkowski, T. P.
- Venue
- PLOS Biology
- Publisher
- Public Library of Science (PLoS)
- Published
- 2019-01-10
- Volume
- 17
- Issue
- 1
- Pages
- e3000107
- ISSN
- 1544-9173 (ISSN-L)
- 1545-7885
- OpenAlex
- W2890617931
- Cited by
- 293
- References
- 29
- FWCI
- 8.16
- Type
- journal article
- Language
- en
- Open access
- gold
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