The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.
DNA Methylation Dynamics in Human Induced Pluripotent Stem Cells over Time
Open accessAbstract
Epigenetic reprogramming is a critical event in the generation of induced pluripotent stem cells (iPSCs). Here, we determined the DNA methylation profiles of 22 human iPSC lines derived from five different cell types (human endometrium, placental artery endothelium, amnion, fetal lung fibroblast, and menstrual blood cell) and five human embryonic stem cell (ESC) lines, and we followed the aberrant methylation sites in iPSCs for up to 42 weeks. The iPSCs exhibited distinct epigenetic differences from ESCs, which were caused by aberrant methylation at early passages. Multiple appearances and then disappearances of random aberrant methylation were detected throughout iPSC reprogramming. Continuous passaging of the iPSCs diminished the differences between iPSCs and ESCs, implying that iPSCs lose the characteristics inherited from the parent cells and adapt to very closely resemble ESCs over time. Human iPSCs were gradually reprogrammed through the "convergence" of aberrant hyper-methylation events that continuously appeared in a de novo manner. This iPS reprogramming consisted of stochastic de novo methylation and selection/fixation of methylation in an environment suitable for ESCs. Taken together, random methylation and convergence are driving forces for long-term reprogramming of iPSCs to ESCs.
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Cites
Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources
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Genome-scale DNA methylation maps of pluripotent and differentiated cells
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Cited by
Interactions between JARID2 and Noncoding RNAs Regulate PRC2 Recruitment to Chromatin
Kaneko, S., Bonasio, R., Saldaña-Meyer, R., Yoshida, T., Son, J., Nishino, K., Umezawa, A., & Reinberg, D. (2014) · Molecular Cell · doi:10.1016/j.molcel.2013.11.012 · Cited by 371
Self-citationGenetic and Epigenetic Variations in iPSCs: Potential Causes and Implications for Application
Liang, G., & Zhang, Y. (2013) · Cell Stem Cell · doi:10.1016/j.stem.2013.07.001 · Cited by 369
Control of intestinal stem cell function and proliferation by mitochondrial pyruvate metabolism
Schell, J. C., Wisidagama, D. R., Bensard, C., Zhao, H., Wei, P., Tanner, J., Flores, A., Mohlman, J., Sorensen, L. K., Earl, C. S., et al. (2017) · Nature Cell Biology · doi:10.1038/ncb3593 · Cited by 322
Epigenetic Regulation of Pluripotency and Differentiation
Boland, M. J., Nazor, K. L., & Loring, J. F. (2014) · Circulation Research · doi:10.1161/circresaha.115.301517 · Cited by 271
The hallmarks of fibroblast ageing
Tigges, J., Krutmann, J., Fritsche, E., Haendeler, J., Schaal, H., Fischer, J. W., Kalfalah, F., Reinke, H., Reifenberger, G., Stühler, K., et al. (2014) · Mechanisms of Ageing and Development · doi:10.1016/j.mad.2014.03.004 · Cited by 250
Metadata
- Authors
- Nishino, K., Toyoda, M., Yamazaki-Inoue, M., Fukawatase, Y., Chikazawa, E., Sakaguchi, H., Akutsu, H., & Umezawa, A.
- Venue
- PLoS Genetics
- Publisher
- Public Library of Science (PLoS)
- Published
- 2011-05-26
- Volume
- 7
- Issue
- 5
- Pages
- e1002085
- ISSN
- 1553-7390 (ISSN-L)
- 1553-7404
- OpenAlex
- W1991059160
- Cited by
- 314
- References
- 48
- FWCI
- 11.40
- Type
- journal article
- Language
- en
- Open access
- gold
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- Nishino, K., Toyoda, M., Yamazaki-Inoue, M., Fukawatase, Y., Chikazawa, E., Sakaguchi, H., Akutsu, H., & Umezawa, A. (2011). DNA Methylation Dynamics in Human Induced Pluripotent Stem Cells over Time. PLoS Genetics. https://doi.org/10.1371/journal.pgen.1002085
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