Electrochemically Generated Acid and Its Containment to 100 Micron Reaction Areas for the Production of DNA Microarrays
Open accessAbstract
An addressable electrode array was used for the production of acid at sufficient concentration to allow deprotection of the dimethoxytrityl (DMT) protecting group from an overlaying substrate bound to a porous reaction layer. Containment of the generated acid to an active electrode of 100 micron diameter was achieved by the presence of an organic base. This procedure was then used for the production of a DNA array, in which synthesis was directed by the electrochemical removal of the DMT group during synthesis. The product array was found to have a detection sensitivity to as low as 0.5 pM DNA in a complex background sample.
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Proton-coupled redox reactions, such as electrochemical oxidation of hydroquinone to benzoquinone, can create the required acidic condition to remove protecting groups.
“It has been reported that proton-coupled redox reactions, such as the electrochemical oxidation of hydroquinone to benzoquinone, can create the required acidic condition to remove the protecting groups (35, 36).”
From Electrochemical DNA synthesis and sequencing on a single electrode with scalability for integrated data storage (2021) · cites this paper as [36]
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- Authors
- Maurer, K., Cooper, J., Caraballo, M., Crye, J., Suciu, D., Ghindilis, A., Leonetti, J. A., Wang, W., Rossi, F. M., Stöver, A. G., Larson, C., Gao, H., Dill, K., & McShea, A.
- Affiliations
- CombiMatrix (US)
- Corresponding author
- Maurer, Karl
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2006-12-20
- Volume
- 1
- Issue
- 1
- Pages
- e34
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W1993110711
- Cited by
- 80
- References
- 16
- Citations by year
- 2026: 5 · 2025: 10 · 2024: 6 · 2023: 8 · 2022: 6 · 2021: 8 · 2020: 1 · 2019: 8 · 2018: 6 · 2017: 1 · 2016: 1 · 2015: 4 · 2014: 1 · 2013: 2 · 2012: 2
- FWCI
- 0.54
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion, cc-by)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
- Keywords
- DNA microarrays, organic bases, DNA synthesis, detection sensitivity
- MeSH terms
- Acids, Base Sequence, Indicators and Reagents, Microchemistry, Microelectrodes, Oligodeoxyribonucleotides, DNA Primers, Oligonucleotide Array Sequence Analysis, Electrochemical Techniques
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