Electrochemically directed synthesis of oligonucleotides for DNA microarray fabrication
Open accessAbstract
We demonstrate a new method for making oligonucleotide microarrays by synthesis in situ. The method uses conventional DNA synthesis chemistry with an electrochemical deblocking step. Acid is delivered to specific regions on a glass slide, thus allowing nucleotide addition only at chosen sites. The acid is produced by electrochemical oxidation controlled by an array of independent microelectrodes. Deblocking is complete in a few seconds, when competing side-product reactions are minimal. We demonstrate the successful synthesis of 17mers and discrimination of single base pair mismatched hybrids. Features generated in this study are 40 mum wide, with sharply defined edges. The synthetic technique may be applicable to fabrication of other molecular arrays.
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Proton-coupled redox reactions, such as electrochemical oxidation of hydroquinone to benzoquinone, can create the required acidic condition to remove protecting groups.
“It has been reported that proton-coupled redox reactions, such as the electrochemical oxidation of hydroquinone to benzoquinone, can create the required acidic condition to remove the protecting groups (35, 36).”
From Electrochemical DNA synthesis and sequencing on a single electrode with scalability for integrated data storage (2021) · cites this paper as [35]
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- Authors
- Egeland, R. D.
- Affiliations
- University of Oxford (GB)
- Corresponding author
- Egeland, R. D.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2005-08-02
- Volume
- 33
- Issue
- 14
- Pages
- e125-e125
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- PMC
- PMC1183109
- OpenAlex
- W2102518893
- Cited by
- 122
- References
- 34
- Citations by year
- 2026: 8 · 2025: 14 · 2024: 10 · 2023: 11 · 2022: 7 · 2021: 6 · 2019: 5 · 2018: 3 · 2017: 1 · 2016: 3 · 2015: 2 · 2014: 2 · 2013: 4 · 2012: 5
- FWCI
- 3.26
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Nucleic Acids Research (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Rhodes Scholarships
- Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Keywords
- oligonucleotide synthesis, DNA microarrays, electrochemical oxidation, microelectrodes, in situ synthesis, molecular arrays
- MeSH terms
- Acids, Electrochemistry, Methods, Microelectrodes, Nucleic Acid Hybridization, Oxidation-Reduction, Oligonucleotide Probes, Oligonucleotide Array Sequence Analysis
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