Cited by 122Advanced biosensing and bioanalysis techniquesDNA and Nucleic Acid ChemistryMicrofluidic and Capillary Electrophoresis Applications

Electrochemically directed synthesis of oligonucleotides for DNA microarray fabrication

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Egeland, R. D.·2005·Nucleic Acids Research

Abstract

We demonstrate a new method for making oligonucleotide microarrays by synthesis in situ. The method uses conventional DNA synthesis chemistry with an electrochemical deblocking step. Acid is delivered to specific regions on a glass slide, thus allowing nucleotide addition only at chosen sites. The acid is produced by electrochemical oxidation controlled by an array of independent microelectrodes. Deblocking is complete in a few seconds, when competing side-product reactions are minimal. We demonstrate the successful synthesis of 17mers and discrimination of single base pair mismatched hybrids. Features generated in this study are 40 mum wide, with sharply defined edges. The synthetic technique may be applicable to fabrication of other molecular arrays.

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Authors
Egeland, R. D.
Affiliations
University of Oxford (GB)
Corresponding author
Egeland, R. D.
Venue
Nucleic Acids Research
Published
2005-08-02
Volume
33
Issue
14
Pages
e125-e125
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
OpenAlex
W2102518893
Cited by
122
References
34
Citations by year
2026: 8 · 2025: 14 · 2024: 10 · 2023: 11 · 2022: 7 · 2021: 6 · 2019: 5 · 2018: 3 · 2017: 1 · 2016: 3 · 2015: 2 · 2014: 2 · 2013: 4 · 2012: 5
FWCI
3.26
Type
journal article
Language
en
Open access
Locations
  • Nucleic Acids Research (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Rhodes Scholarships
  • Medical Research Council
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
  • Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
Keywords
oligonucleotide synthesis, DNA microarrays, electrochemical oxidation, microelectrodes, in situ synthesis, molecular arrays
MeSH terms
Acids, Electrochemistry, Methods, Microelectrodes, Nucleic Acid Hybridization, Oxidation-Reduction, Oligonucleotide Probes, Oligonucleotide Array Sequence Analysis

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