The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
Genetic Profiling Reveals Cross-Contamination and Misidentification of 6 Adenoid Cystic Carcinoma Cell Lines: ACC2, ACC3, ACCM, ACCNS, ACCS and CAC2
Open accessAbstract
Adenoid cystic carcinoma (ACC) is the second most common malignant neoplasm of the salivary glands. Most patients survive more than 5 years after surgery and postoperative radiation therapy. The 10 year survival rate, however, drops to 40%, due to locoregional recurrences and distant metastases. Improving long-term survival in ACC requires the development of more effective systemic therapies based on a better understanding of the biologic behavior of ACC. Much preclinical research in this field involves the use of cultured cells and, to date, several ACC cell lines have been established. Authentication of these cell lines, however, has not been reported. We performed DNA fingerprint analysis on six ACC cell lines using short tandem repeat (STR) examinations and found that all six cell lines had been contaminated with other cells. ACC2, ACC3, and ACCM were determined to be cervical cancer cells (HeLa cells), whereas the ACCS cell line was composed of T24 urinary bladder cancer cells. ACCNS and CAC2 cells were contaminated with cells derived from non-human mammalian species: the cells labeled ACCNS were mouse cells and the CAC2 cells were rat cells. These observations suggest that future studies using ACC cell lines should include cell line authentication to avoid the use of contaminated or non-human cells.
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“Two adenoid cystic carcinoma, ACC-2 and ACC-M were reported as being contaminated by HeLa [22].”
From Investigation of Cross-Contamination and Misidentification of 278 Widely Used Tumor Cell Lines (2017) · cites this paper as [22]
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Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species
Hebert, P. D. N., Ratnasingham, S., & de Waard, J. R. (2003) · Proceedings of the Royal Society of London. Series B: Biological Sciences · doi:10.1098/rsbl.2003.0025 · Cited by 4,466
(1952) · Cancer Research · Cited by 987
Schweppe, R. E., Klopper, J. P., Korch, C., Pugazhenthi, U., Benezra, M., Knauf, J. A., Fagin, J. A., Marlow, L. A., Copland, J. A., Smallridge, R. C., et al. (2008) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2008-1102 · Cited by 563
HeLa cells 50 years on: the good, the bad and the ugly
Masters, J. R. (2002) · Nature Reviews Cancer · doi:10.1038/nrc775 · Cited by 560
Established cell line of urinary bladder carcinoma (T24) containing tumour‐specific antigen
Bubenik, J., Barešová, M., Viklický, V., Jakoubková, J., Sainerová, H., & DONNER, J. (1973) · International Journal of Cancer · doi:10.1002/ijc.2910110327 · Cited by 524
Short tandem repeat profiling provides an international reference standard for human cell lines
Masters, J. R., Thomson, J. A., Daly-Burns, B., Reid, Y. A., Dirks, W. G., Packer, P., Toji, L. H., Ohno, T., Tanabe, H., Arlett, C. F., et al. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.121616198 · Cited by 478
Validity of nucleic acid purities monitored by 260nm/280nm absorbance ratios.
Glasel, J. A. (1995) · PubMed · Cited by 437
The Costs of using Unauthenticated, Over-Passaged Cell Lines: How Much more Data do we Need?
Hughes, P., Marshall, D., Reid, Y., Parkes, H., & Gelber, C. (2007) · BioTechniques · doi:10.2144/000112598 · Cited by 434
Spontaneous transformation and immortalization of human endothelial cells
Takahashi, K., Sawasaki, Y., Hata, J., Mukai, K., & Goto, T. (1990) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/bf02624456 · Cited by 419
Rae, J. M., Creighton, C. J., Meck, J. M., Haddad, B. R., & Johnson, M. D. (2007) · Breast Cancer Research and Treatment · doi:10.1007/s10549-006-9392-8 · Cited by 374
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Research works printed but not linked: 4
- [8]Carlson ER Kim j Mirowski GW Stewart JCB Zarbo RJ 2008 Salivary gland diseases. Oral Pathology: Clinical Pathology Correlations. Regezi JA Sciubba JJ Jordan RCK St. Louis, MI, USA Saunders 179 215
- [16]Takata T Kudo Y Zhao M Ogawa I Miyauchi M 1999 Reduced expression of p27(Kip1) protein in relation to salivary adenoid cystic carcinoma metastasis. Cancer 86 928 35 10491517
- [30]Suda K Rothen-Rutishauser B Günthert M Wunderli-Allenspach H 2001 Phenotypic characterization of human umbilical vein endothelial (ECV304) and urinary carcinoma (T24) cells: endothelial versus epithelial features. In Vitro Cell Dev Biol Anim 37 505 14 11669284
- [37]MacLeod RA Dirks WG Matsuo Y Kaufmann M Milch H 1999 Widespread intraspecies cross-contamination of human tumor cell lines arising at source. Int J Cancer 83 555 63 10508494
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Items 101–105 of 105
Gonçalves, M. T., Lavareze, L., Egal, É. S. A., ALTEMANI, A. M. D. A. M., & Mariano, F. V. (2025) · Cytotechnology · doi:10.1007/s10616-025-00726-6
“The ACC-2, ACC-M, and ACC-3 cell lines were initially described in the 1980s and 1990s, but their usage remained limited until the 2000s when molecular studies revealed that they were cervical cancer cells (N.-N. Liu et al. 2017; Phuchareon et al. 2009; Zhao et al. 2011).”
InfluentialStawarz, K., Gorzelnik, A., Klos, W., Korzon, J., Kissin, F., Bieńkowska‐Pluta, K., Stawarz, G., Rusetska, N., & Zwoliński, J. (2026) · World Journal of Clinical Oncology · doi:10.5306/wjco.v17.i4.116329
Stawarz, K., Rusetska, N., Salamaga, H., Kubala, S., Paczkowska, I., Cwiek, P., Berezowska, A., Jozghorbani, M., Kluebsoongnoen, J., Wilga, M., et al. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.10.07.757230
Chen, C., Zhao, X., Chen, X., & Gao, Y. (2026) · International Journal of Cancer · doi:10.1002/ijc.70771
The Impact of the HeLa Cell in the Field of Otolaryngology: Honoring Henrietta Lacks
Jamil, T. L., Thomas, C. M., Mann, S. E., Johnson, A. P., & Cabrera‐Muffly, C. (2026) · Laryngoscope Investigative Otolaryngology · doi:10.1002/lio2.70593
Metadata
- Authors
- Phuchareon, J., Ohta, Y., Woo, J. M., Eisele, D. W., & Tetsu, O.
- Affiliations
- University of California, San Francisco (US)
- Corresponding author
- Tetsu, Osamu
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2009-06-25
- Volume
- 4
- Issue
- 6
- Pages
- e6040
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC2698276
- OpenAlex
- W2000248995
- Cited by
- 105
- References
- 46
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 2 · 2023: 5 · 2022: 3 · 2021: 3 · 2020: 4 · 2019: 2 · 2018: 10 · 2017: 14 · 2016: 10 · 2015: 5 · 2014: 7 · 2013: 10 · 2012: 9
- FWCI
- 7.44
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Figshare (submittedVersion, cc-by)
- Funders
- Adenoid Cystic Carcinoma Research Foundation
- Johns Hopkins University
- University of California, San Francisco
- Topics
- Health Sciences › Medicine › Surgery › Salivary Gland Tumors Diagnosis and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Identification and Quantification in Food
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
- Keywords
- adenoid cystic carcinoma, cell line contamination, cell line authentication, short tandem repeat profiling, DNA fingerprinting, HeLa cells, T24 cells, salivary gland carcinoma
- MeSH terms
- Alleles, Animals, Urinary Bladder Neoplasms, Carcinoma, Adenoid Cystic, Electron Transport Complex IV, HeLa Cells, Humans, Salivary Gland Neoplasms, Gene Expression Regulation, Neoplastic, Gene Expression Profiling, Cell Line, Tumor, Mice, Rats
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