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Cited by 608Extracellular vesicles in diseaseMicroRNA in disease regulationMesenchymal stem cell research

Microvesicles Derived from Adult Human Bone Marrow and Tissue Specific Mesenchymal Stem Cells Shuttle Selected Pattern of miRNAs

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Collino, Federica, Deregibus, Maria Chiara, Bruno, Stefania, Sterpone, Luca, Aghemo, Giulia, Viltono, Laura, Tetta, Ciro, Camussi, Giovanni·2010·PLoS ONE
DOI 10.1371/journal.pone.0011803PMC PMC2910725
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Abstract

BACKGROUND: Cell-derived microvesicles (MVs) have been described as a new mechanism of cell-to-cell communication. MVs after internalization within target cells may deliver genetic information. Human bone marrow derived mesenchymal stem cells (MSCs) and liver resident stem cells (HLSCs) were shown to release MVs shuttling functional mRNAs. The aim of the present study was to evaluate whether MVs derived from MSCs and HLSCs contained selected micro-RNAs (miRNAs). METHODOLOGY/PRINCIPAL FINDINGS: MVs were isolated from MSCs and HLSCs. The presence in MVs of selected ribonucleoproteins involved in the traffic and stabilization of RNA was evaluated. We observed that MVs contained TIA, TIAR and HuR multifunctional proteins expressed in nuclei and stress granules, Stau1 and 2 implicated in the transport and stability of mRNA and Ago2 involved in miRNA transport and processing. RNA extracted from MVs and cells of origin was profiled for 365 known human mature miRNAs by real time PCR. Hierarchical clustering and similarity analysis of miRNAs showed 41 co-expressed miRNAs in MVs and cells. Some miRNAs were accumulated within MVs and absent in the cells after MV release; others were retained within the cells and not secreted in MVs. Gene ontology analysis of predicted and validated targets showed that the high expressed miRNAs in cells and MVs could be involved in multi-organ development, cell survival and differentiation. Few selected miRNAs shuttled by MVs were also associated with the immune system regulation. The highly expressed miRNAs in MVs were transferred to target cells after MV incorporation. CONCLUSIONS: This study demonstrated that MVs contained ribonucleoproteins involved in the intracellular traffic of RNA and selected pattern of miRNAs, suggesting a dynamic regulation of RNA compartmentalization in MVs. The observation that MV-highly expressed miRNAs were transferred to target cells, rises the possibility that the biological effect of stem cells may, at least in part, depend on MV-shuttled miRNAs. Data generated from this study, stimulate further functional investigations on the predicted target genes and pathways involved in the biological effect of human adult stem cells.

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  • microRNA's presence in exosomes has been reported in different cells and cancer types.

    “microRNA's presence in exosomes have been already reported in different cells and cancer types (44–50).”

    From Potential Therapeutic Effects of Exosomes Packed With a miR-21-Sponge Construct in a Rat Model of Glioblastoma (2019) · cites this paper as [44]

    The Introduction (page not available) reports miRNAs in mast-cell exosomes and in microvesicles from several other cell sources, including the blood of patients with ovarian tumors. It does not establish their presence in exosomes across different cancer types; the tumor-related example concerns blood microvesicles, not identified cancer-cell exosomes. (LLM, conf=0.85)

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Authors
Collino, F., Deregibus, M. C., Bruno, S., Sterpone, L., Aghemo, G., Viltono, L., Tetta, C., & Camussi, G.
Affiliations
  • University of Turin (IT)
  • Politecnico di Torino (IT)
  • Fresenius Medical Care (Germany) (DE)
Corresponding author
Camussi, Giovanni
ORCID
Venue
PLoS ONE
Publisher
Public Library of Science (PLoS)
Published
2010-07-27
Volume
5
Issue
7
Pages
e11803
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0011803
PMC
PMC2910725
OpenAlex
W2108541168
Cited by
608
References
3
Citations by year
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FWCI
20.73
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Institutional Research Information System University of Turin (University of Turin) (submittedVersion, other-oa)
  • Padua Research Archive (University of Padova) (submittedVersion, cc-by)
  • Archivio Istituzionale della Ricerca (Universita Degli Studi Di Milano) (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
  • PORTO@Iris (submittedVersion, cc-by)
  • PORTO Publications Open Repository TOrino (Politecnico di Torino) (submittedVersion)
Funders
  • Fresenius Medical Care North America
  • Associazione Italiana per la Ricerca sul Cancro
  • Regione Piemonte
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
Keywords
microvesicles, mesenchymal stem cells, bone marrow, microRNA, extracellular vesicles, ribonucleoprotein, transient ischemic attack, TIAR, HuR, STAU1, STAU2, Argonaute 2, cell-to-cell communication, immune regulation
MeSH terms
Bone Marrow, Cells, Cultured, Cytoskeleton, Humans, Ribonucleoproteins, Stem Cells, Blotting, Western, In Situ Hybridization, Reverse Transcriptase Polymerase Chain Reaction, Cytoplasmic Vesicles, MicroRNAs, Mesenchymal Stem Cells

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