The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.
Multiplexed DNA Sequence Capture of Mitochondrial Genomes Using PCR Products
Open accessAbstract
BACKGROUND: To utilize the power of high-throughput sequencers, target enrichment methods have been developed. The majority of these require reagents and equipment that are only available from commercial vendors and are not suitable for the targets that are a few kilobases in length. METHODOLOGY/PRINCIPAL FINDINGS: We describe a novel and economical method in which custom made long-range PCR products are used to capture complete human mitochondrial genomes from complex DNA mixtures. We use the method to capture 46 complete mitochondrial genomes in parallel and we sequence them on a single lane of an Illumina GA(II) instrument. CONCLUSIONS/SIGNIFICANCE: This method is economical and simple and particularly suitable for targets that can be amplified by PCR and do not contain highly repetitive sequences such as mtDNA. It has applications in population genetics and forensics, as well as studies of ancient DNA.
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<tt>BLAT</tt> —The <tt>BLAST</tt> -Like Alignment Tool
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Target-enrichment strategies for next-generation sequencing
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Genome-wide in situ exon capture for selective resequencing
Hodges, E., Xuan, Z., Balija, V., Kramer, M., Molla, M. N., Smith, S. W., Middle, C. M., Rodesch, M. J., Albert, T. J., Hannon, G. J., et al. (2007) · Nature Genetics · doi:10.1038/ng.2007.42 · Cited by 677
A Complete Neandertal Mitochondrial Genome Sequence Determined by High-Throughput Sequencing
Green, R. E., Malaspinas, A.-S., Krause, J., Briggs, A. W., Johnson, P. L. F., Uhler, C., Meyer, M., Good, J. M., Maricic, T., Stenzel, U., et al. (2008) · Cell · doi:10.1016/j.cell.2008.06.021 · Cited by 599
Self-citationMeyerhans, A., Vartanian, J.-P., & Wain-Hobson, S. (1990) · Nucleic Acids Research · doi:10.1093/nar/18.7.1687 · Cited by 574
Heteroplasmic mitochondrial DNA mutations in normal and tumour cells
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Targeted Retrieval and Analysis of Five Neandertal mtDNA Genomes
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Self-citationMITOCHONDRIAL DNA AND HUMAN EVOLUTION
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Cited by
Gut microbiota, metabolites and host immunity
Rooks, M. G., & Garrett, W. S. (2016) · Nature reviews. Immunology · doi:10.1038/nri.2016.42 · Cited by 3,409
Genetic history of an archaic hominin group from Denisova Cave in Siberia
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Self-citationAncient human genomes suggest three ancestral populations for present-day Europeans
Lazaridis, I., Patterson, N., Mittnik, A., Renaud, G., Mallick, S., Kirsanow, K., Sudmant, P. H., Schraiber, J. G., Castellano, S., Lipson, M., et al. (2014) · Nature · doi:10.1038/nature13673 · Cited by 1,357
Self-citationDouble indexing overcomes inaccuracies in multiplex sequencing on the Illumina platform
Kircher, M., Sawyer, S., & Meyer, M. (2012) · Nucleic Acids Research · doi:10.1093/nar/gkr771 · Cited by 1,106
“Second, the same libraries were enriched individually for mitochondrial DNA using a recently published hybridization capture method (21) and then pooled for sequencing (experiment SP-CAP).”
Method
Metadata
- Authors
- Maricic, T., Whitten, M., & Pääbo, S.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2010-11-16
- Volume
- 5
- Issue
- 11
- Pages
- e14004
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W1975574050
- Cited by
- 565
- References
- 16
- FWCI
- 10.63
- Type
- journal article
- Language
- en
- Open access
- gold
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