The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
Global Changes in Staphylococcus aureus Gene Expression in Human Blood
Open accessAbstract
Staphylococcus aureus is a leading cause of bloodstream infections worldwide. In the United States, many of these infections are caused by a strain known as USA300. Although progress has been made, our understanding of the S. aureus molecules that promote survival in human blood and ultimately facilitate metastases is incomplete. To that end, we analyzed the USA300 transcriptome during culture in human blood, human serum, and trypticase soy broth (TSB), a standard laboratory culture media. Notably, genes encoding several cytolytic toxins were up-regulated in human blood over time, and hlgA, hlgB, and hlgC (encoding gamma-hemolysin subunits HlgA, HlgB, and HlgC) were among the most highly up-regulated genes at all time points. Compared to culture supernatants from a wild-type USA300 strain (LAC), those derived from an isogenic hlgABC-deletion strain (LACΔhlgABC) had significantly reduced capacity to form pores in human neutrophils and ultimately cause neutrophil lysis. Moreover, LACΔhlgABC had modestly reduced ability to cause mortality in a mouse bacteremia model. On the other hand, wild-type and LACΔhlgABC strains caused virtually identical abscesses in a mouse skin infection model, and bacterial survival and neutrophil lysis after phagocytosis in vitro was similar between these strains. Comparison of the cytolytic capacity of culture supernatants from wild-type and isogenic deletion strains lacking hlgABC, lukS/F-PV (encoding PVL), and/or lukDE revealed functional redundancy among two-component leukotoxins in vitro. These findings, along with a requirement of specific growth conditions for leukotoxin expression, may explain the apparent limited contribution of any single two-component leukotoxin to USA300 immune evasion and virulence.
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Klevens, R. M. (2007) · JAMA · doi:10.1001/jama.298.15.1763 · Cited by 3,726 · cited 3×
“the United State and Canada [3–7].”
Waves of resistance: Staphylococcus aureus in the antibiotic era
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Self-citationAndrews, S. C., Robinson, A. K., & Rodríguez-Quiñones, F. (2003) · FEMS Microbiology Reviews · doi:10.1016/s0168-6445(03)00055-x · Cited by 2,702 · cited 1×
“0 mM [11,28].”
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Self-citationPassage of Heme-Iron Across the Envelope of Staphylococcus aureus
Mazmanian, S. K., Skaar, E. P., Gaspar, A. H., Humayun, M., Gornicki, P., Jelenska, J., Joachmiak, A., Missiakas, D. M., & Schneewind, O. (2003) · Science · doi:10.1126/science.1081147 · Cited by 593
Insights into Mechanisms Used by Staphylococcus aureus to Avoid Destruction by Human Neutrophils
Voyich, J. M., Braughton, K. R., Sturdevant, D. E., Whitney, A. R., Saïd-Salim, B., Porcella, S. F., Long, R. D., Dorward, D. W., Gardner, D. J., Kreiswirth, B. N., et al. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.175.6.3907 · Cited by 563
Self-citationIron-Source Preference of Staphylococcus aureus Infections
Skaar, E. P., Humayun, M., Bae, T., DeBord, K. L., & Schneewind, O. (2004) · Science · doi:10.1126/science.1099930 · Cited by 440
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Self-citationDale, S. E., Doherty-Kirby, A., Lajoie, G., & Heinrichs, D. E. (2004) · Infection and Immunity · doi:10.1128/iai.72.1.29-37.2004 · Cited by 232
Cited by
Predictors of Mortality in Staphylococcus aureus Bacteremia
van Hal, S. J., Jensen, S. O., Vaska, V. L., Espedido, B. A., Paterson, D. L., & Gosbell, I. B. (2012) · Clinical Microbiology Reviews · doi:10.1128/cmr.05022-11 · Cited by 932
Staphylococcus aureus Secreted Toxins and Extracellular Enzymes
Tam, K., & Torres, V. J. (2019) · Microbiology Spectrum · doi:10.1128/microbiolspec.gpp3-0039-2018 · Cited by 452
Sully, E. K., Malachowa, N., Elmore, B. O., Alexander, S. M., Femling, J. K., Gray, B. M., DeLeo, F. R., Otto, M., Cheung, A. L., Edwards, B. S., et al. (2014) · PLoS Pathogens · doi:10.1371/journal.ppat.1004174 · Cited by 386
Self-citationThe Bicomponent Pore-Forming Leucocidins of Staphylococcus aureus
Alonzo, F., & Torres, V. J. (2014) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00055-13 · Cited by 276
Recent developments in understanding the iron acquisition strategies of gram positive pathogens
Sheldon, J. R., & Heinrichs, D. E. (2015) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuv009 · Cited by 272
Metadata
- Authors
- Malachowa, N., Whitney, A. R., Kobayashi, S. D., Sturdevant, D. E., Kennedy, A. D., Braughton, K. R., Shabb, D. W., Diep, B. A., Chambers, H. F., Otto, M., & DeLeo, F. R.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2011-04-15
- Volume
- 6
- Issue
- 4
- Pages
- e18617
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2048580389
- Cited by
- 230
- References
- 11
- FWCI
- 13.61
- Type
- journal article
- Language
- en
- Open access
- gold
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- Malachowa, N., Whitney, A. R., Kobayashi, S. D., Sturdevant, D. E., Kennedy, A. D., Braughton, K. R., Shabb, D. W., Diep, B. A., Chambers, H. F., Otto, M., & DeLeo, F. R. (2011). Global Changes in Staphylococcus aureus Gene Expression in Human Blood. PLoS ONE. https://doi.org/10.1371/journal.pone.0018617
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
