The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Daptomycin Resistance in Enterococci Is Associated with Distinct Alterations of Cell Membrane Phospholipid Content
Open accessAbstract
BACKGROUND: The lipopeptide antibiotic, daptomycin (DAP) interacts with the bacterial cell membrane (CM). Development of DAP resistance during therapy in a clinical strain of Enterococcus faecalis was associated with mutations in genes encoding enzymes involved in cell envelope homeostasis and phospholipid metabolism. Here we characterized changes in CM phospholipid profiles associated with development of DAP resistance in clinical enterococcal strains. METHODOLOGY: Using two clinical strain-pairs of DAP-susceptible and DAP-resistant E. faecalis (S613 vs. R712) and E. faecium (S447 vs. R446) recovered before and after DAP therapy, we compared four distinct CM profiles: phospholipid content, fatty acid composition, membrane fluidity and capacity to be permeabilized and/or depolarized by DAP. Additionally, we characterized the cell envelope of the E. faecium strain-pair by transmission electron microscopy and determined the relative cell surface charge of both strain-pairs. PRINCIPAL FINDINGS: Both E. faecalis and E. faecium mainly contained four major CM PLs: phosphatidylglycerol (PG), cardiolipin, lysyl-phosphatidylglycerol (L-PG) and glycerolphospho-diglycodiacylglycerol (GP-DGDAG). In addition, E. faecalis CMs (but not E. faecium) also contained: i) phosphatidic acid; and ii) two other unknown species of amino-containing PLs. Development of DAP resistance in both enterococcal species was associated with a significant decrease in CM fluidity and PG content, with a concomitant increase in GP-DGDAG. The strain-pairs did not differ in their outer CM translocation (flipping) of amino-containing PLs. Fatty acid content did not change in the E. faecalis strain-pair, whereas a significant decrease in unsaturated fatty acids was observed in the DAP-resistant E. faecium isolate R446 (vs S447). Resistance to DAP in E. faecium was associated with distinct structural alterations of the cell envelope and cell wall thickening, as well as a decreased ability of DAP to depolarize and permeabilize the CM. CONCLUSION: Distinct alterations in PL content and fatty acid composition are associated with development of enterococcal DAP resistance.
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Hidron, A. I., Edwards, J. R., Patel, J., Horan, T. C., Sievert, D. M., Pollock, D. A., Fridkin, S. K., & National Healthcare Safety Network Team and Participating National Healthcare Safety Network Facilities (2008) · Infection Control & Hospital Epidemiology · doi:10.1086/591861 · Cited by 3,724
Membrane lipid homeostasis in bacteria
Zhang, Y.-M., & Rock, C. O. (2008) · Nature Reviews Microbiology · doi:10.1038/nrmicro1839 · Cited by 1,393 · cited 5×
“coli, the balance of zwitterionic and acidic (negatively-charged) PLs is the result of the formation of PG, which is tightly coupled with the regulation of the synthesis of other PLs [26].”
BackgroundInfluentialGenetic Basis for In Vivo Daptomycin Resistance in Enterococci
Arias, C. A., Panesso, D., McGrath, D. M., Qin, X., Mojica, M. F., Miller, C., Diaz, L., Tran, T. T., Rincon, S., Barbu, E. M., et al. (2011) · New England Journal of Medicine · doi:10.1056/nejmoa1011138 · Cited by 377
Self-citationPogliano, J., Pogliano, N., & Silverman, J. A. (2012) · Journal of Bacteriology · doi:10.1128/jb.00011-12 · Cited by 319
Bayer, A. S., Prasad, R., Chandra, J., Koul, A., Smriti, M., Varma, A., Skurray, R. A., Firth, N., Brown, M. H., Koo, S.-P., et al. (2000) · Infection and Immunity · doi:10.1128/iai.68.6.3548-3553.2000 · Cited by 150 · cited 2×
“The protocol followed previously-published techniques for DPH incorporation into target CMs, measurement of fluorescence polarization and calculation of the degree of fluorescence polarization (polarization index) [46].”
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Cited by
Molecular mechanisms of antibiotic resistance revisited
Darby, E. M., Trampari, E., Siasat, P., Gaya, M. S., Alav, I., Webber, M. A., & Blair, J. M. A. (2023) · Nature Reviews Microbiology · doi:10.1038/s41579-022-00820-y · Cited by 1,398
Mechanisms of antibiotic resistance in enterococci
Miller, W. R., Munita, J. M., & Arias, C. A. (2014) · Expert Review of Anti-infective Therapy · doi:10.1586/14787210.2014.956092 · Cited by 772
Self-citationAntimicrobial Peptides Targeting Gram-Positive Bacteria
Malanovic, N., & Lohner, K. (2016) · Pharmaceuticals · doi:10.3390/ph9030059 · Cited by 420
Tran, T. T., Panesso, D., Mishra, N. N., Mileykovskaya, E., Guan, Z., Munita, J. M., Reyes, J., Diaz, L., Weinstock, G. M., Murray, B. E., et al. (2013) · mBio · doi:10.1128/mbio.00281-13 · Cited by 192
Self-citationTran, T. T., Panesso, D., Gao, H., Roh, J. H., Munita, J. M., Reyes, J., Diaz, L., Lobos, E. A., Shamoo, Y., Mishra, N. N., et al. (2013) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01454-12 · Cited by 125
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Metadata
- Authors
- Mishra, N. N., Bayer, A. S., Tran, T. T., Shamoo, Y., Mileykovskaya, E., Dowhan, W., Guan, Z., & Arias, C. A.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2012-08-27
- Volume
- 7
- Issue
- 8
- Pages
- e43958
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W1978631761
- Cited by
- 168
- References
- 5
- FWCI
- 4.47
- Type
- journal article
- Language
- en
- Open access
- gold
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- Mishra, N. N., Bayer, A. S., Tran, T. T., Shamoo, Y., Mileykovskaya, E., Dowhan, W., Guan, Z., & Arias, C. A. (2012). Daptomycin Resistance in Enterococci Is Associated with Distinct Alterations of Cell Membrane Phospholipid Content. PLoS ONE. https://doi.org/10.1371/journal.pone.0043958
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