The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Assessing the Fecal Microbiota: An Optimized Ion Torrent 16S rRNA Gene-Based Analysis Protocol
Open accessAbstract
Assessing the distribution of 16S rRNA gene sequences within a biological sample represents the current state-of-the-art for determination of human gut microbiota composition. Advances in dissecting the microbial biodiversity of this ecosystem have very much been dependent on the development of novel high-throughput DNA sequencing technologies, like the Ion Torrent. However, the precise representation of this bacterial community may be affected by the protocols used for DNA extraction as well as by the PCR primers employed in the amplification reaction. Here, we describe an optimized protocol for 16S rRNA gene-based profiling of the fecal microbiota.
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Cites
QIIME allows analysis of high-throughput community sequencing data
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An obesity-associated gut microbiome with increased capacity for energy harvest
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A human gut microbial gene catalogue established by metagenomic sequencing
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“The Ion Torrent PGM instrument represents a recently commercialized bench-top NGS platform and is marketed as being less costly and with a faster turnaround as compared to other NGS techniques such as the 454 and Illumina platforms [10,11].”
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Cited by
Milani, C., Duranti, S., Bottacini, F., Casey, E., Turroni, F., Mahony, J., Belzer, C., Delgado Palacio, S., Arboleya Montes, S., Mancabelli, L., et al. (2017) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00036-17 · Cited by 1,950
Self-citationProbiotics, gut microbiota, and their influence on host health and disease
Sánchez, B., Delgado, S., Blanco‐Míguez, A., Lourenço, A., Gueimonde, M., & Margolles, A. (2017) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.201600240 · Cited by 1,104
Self-citationA new view of the fish gut microbiome: advances from next-generation sequencing
Ghanbari, M., Kneifel, W., & Domig, K. J. (2015) · Aquaculture · doi:10.1016/j.aquaculture.2015.06.033 · Cited by 669
Intestinal Dysbiosis Associated with Systemic Lupus Erythematosus
Hevia, A., Milani, C., López, P., Cuervo, A., Arboleya, S., Duranti, S., Turroni, F., González, S., Suárez, A., Gueimonde, M., et al. (2014) · mBio · doi:10.1128/mbio.01548-14 · Cited by 622
Self-citationBertocchi, A., Carloni, S., Ravenda, P. S., Bertalot, G., Spadoni, I., Lo Cascio, A., Gandini, S., Lizier, M., Braga, D., Asnicar, F., et al. (2021) · Cancer Cell · doi:10.1016/j.ccell.2021.03.004 · Cited by 515
Metadata
- Authors
- Milani, C., Hevia, A., Foroni, E., Duranti, S., Turroni, F., Lugli, G. A., Sanchez, B., Martín, R., Gueimonde, M., van Sinderen, D., Margolles, A., & Ventura, M.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2013-07-15
- Volume
- 8
- Issue
- 7
- Pages
- e68739
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2059198752
- Cited by
- 307
- References
- 14
- FWCI
- 8.61
- Type
- journal article
- Language
- en
- Open access
- gold
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