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Generation of 3D Skin Equivalents Fully Reconstituted from Human Induced Pluripotent Stem Cells (iPSCs)
Open accessAbstract
Recent generation of patient-specific induced pluripotent stem cells (PS-iPSCs) provides significant advantages for cell- and gene-based therapy. Establishment of iPSC-based therapy for skin diseases requires efficient methodology for differentiating iPSCs into both keratinocytes and fibroblasts, the major cellular components of the skin, as well as the reconstruction of skin structures using these iPSC-derived skin components. We previously reported generation of keratinocytes from human iPSCs for use in the treatment of recessive dystrophic epidermolysis bullosa (RDEB) caused by mutations in the COL7A1 gene. Here, we developed a protocol for differentiating iPSCs into dermal fibroblasts, which also produce type VII collagen and therefore also have the potential to treat RDEB. Moreover, we generated in vitro 3D skin equivalents composed exclusively human iPSC-derived keratinocytes and fibroblasts for disease models and regenerative therapies for skin diseases, first demonstrating that iPSCs can provide the basis for modeling a human organ derived entirely from two different types of iPSC-derived cells.
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“However, human skin equivalents have notable drawbacks, such as a lack of crucial skin tissue structures (e.g., hair follicles and sebaceous glands) and limited culture durations (e.g., 2–3 weeks)”
From Generation and characterization of hair-bearing skin organoids from human pluripotent stem cells (2022) · cites this paper as [19]
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“Furthermore, in response to challenges associated with donor variability, conventional primary cell-based HSEs have transitioned to more standardized and reproducible in vitro culture models. These models utilize either immortalized cell lines [45] or cells derived from hiPSCs [17].”
From Diversity of human skin three-dimensional organotypic cultures (2024) · cites this paper as [17]
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“Reconstruction of appendage-bearing skin in cultures and in bioengineered grafts remains an unmet biomedical challenge3–9.”
From Hair-bearing human skin generated entirely from pluripotent stem cells (2020) · cites this paper as [6]
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“To date, in vitro skin derivation strategies have focused on generating keratinocytes and fibroblasts from PSCs in separate cultures first, then combining the two types of cells to form a skin-like bilayer (Gledhill et al., 2015; Itoh et al., 2013; Oh et al., 2013; Sun et al., 2014).”
From Hair Follicle Development in Mouse Pluripotent Stem Cell-Derived Skin Organoids (2018) · cites this paper as Itoh et al., 2013
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“The logic of these approaches is that the individual parts can be combined to generate full-thickness skin (Itoh et al., 2013).”
From Hair Follicle Development in Mouse Pluripotent Stem Cell-Derived Skin Organoids (2018) · cites this paper as Itoh et al., 2013
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“and that healthy cells can be generated from reprogrammed cells from patients with RDEB (153, 154).”
From Wound repair and regeneration: Mechanisms, signaling, and translation (2014) · cites this paper as [153]
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“It has been shown that human skin equivalents can be created entirely from fibroblasts and keratinocytes generated from patients’ iPSCs, and that healthy cells can be generated from reprogrammed cells from patients with RDEB (153, 154).”
From Wound repair and regeneration: Mechanisms, signaling, and translation (2014) · cites this paper as [153]
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Cites
Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., & Yamanaka, S. (2007) · Cell · doi:10.1016/j.cell.2007.11.019 · Cited by 20,221
Induction of Midbrain Dopaminergic Neurons from ES Cells by Stromal Cell–Derived Inducing Activity
Kawasaki, H., Mizuseki, K., Nishikawa, S., Kaneko, S., Kuwana, Y., Nakanishi, S., Nishikawa, S., & Sasai, Y. (2000) · Neuron · doi:10.1016/s0896-6273(00)00083-0 · Cited by 1,277
Embryonic stem cell differentiation: emergence of a new era in biology and medicinePreprint
Keller, G. M. (2005) · Genes & Development · doi:10.1101/gad.1303605 · Cited by 1,174
Genetic engineering of human pluripotent cells using TALE nucleases
Hockemeyer, D. F., Wang, H., Kiani, S., Lai, C. S., Gao, Q., Cassady, J. P., Cost, G. J., Zhang, L., Santiago, Y., Miller, J. C., et al. (2011) · Nature Biotechnology · doi:10.1038/nbt.1927 · Cited by 1,161
Schuldiner, M., Yanuka, O., Itskovitz‐Eldor, J., Melton, D. A., & Benvenisty, N. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.21.11307 · Cited by 1,132
Targeted gene correction of α1-antitrypsin deficiency in induced pluripotent stem cells
Yusa, K., Rashid, S. T., Strick‐Marchand, H., Varela, I., Liu, P.-Q., Paschon, D. E., Miranda, E., Ordóñez, A., Hannan, N. R. F., Rouhani, F. J., et al. (2011) · Nature · doi:10.1038/nature10424 · Cited by 647
Gene Targeting of a Disease-Related Gene in Human Induced Pluripotent Stem and Embryonic Stem Cells
Zou, J., Maeder, M. L., Mali, P., Pruett‐Miller, S. M., Thibodeau-Beganny, S., Chou, B.-K., Chen, G., Ye, Z., Park, I., Daley, G. Q., et al. (2009) · Cell stem cell · doi:10.1016/j.stem.2009.05.023 · Cited by 513
Prolyl 4-hydroxylases, the key enzymes of collagen biosynthesis
Myllyharju, J. (2003) · Matrix Biology · doi:10.1016/s0945-053x(03)00006-4 · Cited by 500
Ascorbic Acid Enhances Differentiation of Embryonic Stem Cells Into Cardiac Myocytes
Takahashi, T., Lord, B., Schulze, P. C., Fryer, R. M., Sarang, S. S., Gullans, S. R., & Lee, R. T. (2003) · Circulation · doi:10.1161/01.cir.0000064899.53876.a3 · Cited by 478
Roles of TGF-β family signaling in stem cell renewal and differentiation
Watabe, T., & Miyazono, K. (2008) · Cell Research · doi:10.1038/cr.2008.323 · Cited by 446
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Self-citationHair-bearing human skin generated entirely from pluripotent stem cells
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Marx, U. (2016) · ALTEX · doi:10.14573/altex.1603161 · Cited by 295
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Sierra-Sánchez, Á., Kim, K. H., Blasco‐Morente, G., & Arias‐Santiago, S. (2021) · npj Regenerative Medicine · doi:10.1038/s41536-021-00144-0 · Cited by 152
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Lee, J., van der Valk, W. H., Serdy, S. A., Deakin, C., Kim, J., Le, A. P., & Koehler, K. R. (2022) · Nature Protocols · doi:10.1038/s41596-022-00681-y · Cited by 132
Gledhill, K., Guo, Z., Umegaki-Arao, N., Higgins, C. A., Itoh, M., & Christiano, A. M. (2015) · PLOS ONE · doi:10.1371/journal.pone.0136713 · Cited by 132
Self-citationUmegaki-Arao, N., Pasmooij, A. M. G., Itoh, M., Cerise, J. E., Guo, Z., Levy, B., Gostyński, A., Rothman, L. R., Jonkman, M. F., & Christiano, A. M. (2014) · Science Translational Medicine · doi:10.1126/scitranslmed.3009342 · Cited by 123
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Self-citationvan den Broek, L. J., Bergers, L. L., Reijnders, C. M. A., & Gibbs, S. (2017) · Stem Cell Reviews and Reports · doi:10.1007/s12015-017-9737-1 · Cited by 118
Petrova, A., Celli, A., Jacquet, L., Dafou, D., Crumrine, D. A., Hupe, M., Arno, M. J., Hobbs, C., Čvoro, A., Karagiannis, P., et al. (2014) · Stem Cell Reports · doi:10.1016/j.stemcr.2014.03.009 · Cited by 112
Shinkuma, S., Guo, Z., & Christiano, A. M. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1512028113 · Cited by 108
Self-citationKim, Y., Park, N., Rim, Y. A., Nam, Y., Jung, H., Lee, K.-J., & Ju, J. H. (2018) · Stem Cell Research & Therapy · doi:10.1186/s13287-018-0958-2 · Cited by 115
Jarrige, M., Frank, E., Herardot, E., Martineau, S., Darle, A., Benabidès, M., Domingues, S., Chose, O., Habeler, W., Lorant, J., et al. (2021) · Cells · doi:10.3390/cells10020240 · Cited by 107
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Abaci, H., Guo, Z., Doucet, Y., Jacków, J., & Christiano, A. (2017) · Experimental Biology and Medicine · doi:10.1177/1535370217712690 · Cited by 104
Self-citationDystrophic epidermolysis bullosa: a review
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Zielins, E. R., Atashroo, D. A., Maan, Z. N., Duscher, D., Walmsley, G. G., Marecic, O., Hu, M. S.-M., Senarath-Yapa, K., McArdle, A., Tevlin, R., et al. (2014) · Regenerative Medicine · doi:10.2217/rme.14.54 · Cited by 99
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Roy, B., Yuan, L., Lee, Y., Bharti, A., Mitra, A., & Shivashankar, G. V. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1911497117 · Cited by 92
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Veraitch, O. K., Mabuchi, Y., Matsuzaki, Y., Sasaki, T., Okuno, H., Tsukashima, A., Amagai, M., Okano, H., & Ohyama, M. (2017) · Scientific Reports · doi:10.1038/srep42777 · Cited by 69
Yan, Y. C., Wu, R., Bo, Y., Zhang, M., Chen, Y., Wang, X., Huang, M., Liu, B., & Zhang, L. (2020) · Theranostics · doi:10.7150/thno.46639 · Cited by 66
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Choi, K.-Y. G., Wu, B. C., Lee, A. H., Baquir, B., & Hancock, R. E. W. (2020) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2020.00228 · Cited by 54
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Conese, M., Annacontini, L., Carbone, A., Beccia, E., Cecchino, L. R., Parisi, D., Gioia, S. D., Lembo, F., Angiolillo, A., Mastrangelo, F., et al. (2020) · Stem Cells International · doi:10.1155/2020/7056261 · Cited by 55
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Kajiwara, K., Tanemoto, T., Wada, S., Karibe, J., Ihara, N., Ikemoto, Y., Kawasaki, T., Oishi, Y., Samura, O., Okamura, K., et al. (2017) · Stem Cell Reports · doi:10.1016/j.stemcr.2017.05.013 · Cited by 51
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Ohyama, M. (2019) · Inflammation and Regeneration · doi:10.1186/s41232-019-0093-1 · Cited by 46
Matei, A., Chen, C., Kiesewetter, L., Györfi, A., Li, Y., Trinh‐Minh, T., Xu, X., Manh, C. T., Kuppevelt, T. H. V., Hansmann, J., et al. (2019) · Annals of the Rheumatic Diseases · doi:10.1136/annrheumdis-2019-216108 · Cited by 47
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Metadata
- Authors
- Itoh, M., Umegaki-Arao, N., Guo, Z., Liu, L., Higgins, C. A., & Christiano, A. M.
- Affiliations
- Columbia University (US)
- Corresponding author
- Christiano, Angela M.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2013-10-11
- Volume
- 8
- Issue
- 10
- Pages
- e77673
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3795682
- OpenAlex
- W1981286729
- Cited by
- 239
- References
- 42
- Citations by year
- 2026: 11 · 2025: 14 · 2024: 21 · 2023: 16 · 2022: 24 · 2021: 22 · 2020: 18 · 2019: 20 · 2018: 25 · 2017: 23 · 2016: 10 · 2015: 16 · 2014: 18 · 2013: 1
- FWCI
- 6.71
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion, cc-by)
- Columbia Academic Commons (Columbia University)
- Funders
- New York State Foundation for Science, Technology and Innovation
- Leona M. and Harry B. Helmsley Charitable Trust
- New York State Stem Cell Science
- Stanford Diabetes Research Center
- National Institutes of Health (U18TR000561)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (P30AR044535)
- National Center for Advancing Translational Sciences
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
- Health Sciences › Medicine › Dermatology › Hair Growth and Disorders
- Keywords
- induced pluripotent stem cells, 3D skin equivalents, dermal fibroblasts, keratinocytes, recessive dystrophic epidermolysis bullosa, COL7A1, type VII collagen, iPSC differentiation, regenerative therapy, patient-derived iPSCs
- MeSH terms
- Cell Differentiation, Cells, Cultured, Fibroblasts, Flow Cytometry, Humans, Skin, Blotting, Western, Keratinocytes, Reverse Transcriptase Polymerase Chain Reaction, Induced Pluripotent Stem Cells
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