Chlorogenic Acid Inhibits Human Platelet Activation and Thrombus Formation
Open accessAbstract
BACKGROUND: Chlorogenic acid is a potent phenolic antioxidant. However, its effect on platelet aggregation, a critical factor in arterial thrombosis, remains unclear. Consequently, chlorogenic acid-action mechanisms in preventing platelet activation and thrombus formation were examined. METHODS AND RESULTS: Chlorogenic acid in a dose-dependent manner (0.1 to 1 mmol/L) inhibited platelet secretion and aggregation induced by ADP, collagen, arachidonic acid and TRAP-6, and diminished platelet firm adhesion/aggregation and platelet-leukocyte interactions under flow conditions. At these concentrations chlorogenic acid significantly decreased platelet inflammatory mediators (sP-selectin, sCD40L, CCL5 and IL-1β) and increased intraplatelet cAMP levels/PKA activation. Interestingly, SQ22536 (an adenylate cyclase inhibitor) and ZM241385 (a potent A2A receptor antagonist) attenuated the antiplatelet effect of chlorogenic acid. Chlorogenic acid is compatible to the active site of the adenosine A2A receptor as revealed through molecular modeling. In addition, chlorogenic acid had a significantly lower effect on mouse bleeding time when compared to the same dose of aspirin. CONCLUSIONS: Antiplatelet and antithrombotic effects of chlorogenic acid are associated with the A2A receptor/adenylate cyclase/cAMP/PKA signaling pathway.
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Polyphenols such as chlorogenic acid or the anthocyanin cyanidin-3-glucoside interrupt thrombin-induced platelet aggregation.
“In vitro and in vivo studies showed that polyphenols such as chlorogenic acid or the anthocyanin cyanidin-3-glucoside interrupt thrombin-induced platelet aggregation [60,61].”
From Mediterranean Diet as an Antioxidant: The Impact on Metabolic Health and Overall Wellbeing (2021) · cites this paper as [60]
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Mediterranean Diet as an Antioxidant: The Impact on Metabolic Health and Overall Wellbeing
Gantenbein, K. V., & Kanaka-Gantenbein, C. (2021) · Nutrients · doi:10.3390/nu13061951 · Cited by 288 · see citing works
Polyphenols: Modulators of Platelet Function and Platelet Microparticle Generation?
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- Authors
- Fuentes, E., Caballero, J., Alarcón, M., Rojas, A., & Palomo, I.
- Affiliations
- Universidad de Talca (CL)
- Corresponding author
- Palomo, Iván
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2014-03-05
- Volume
- 9
- Issue
- 3
- Pages
- e90699
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3944540
- OpenAlex
- W2014198498
- Cited by
- 115
- References
- 57
- Citations by year
- 2026: 5 · 2025: 7 · 2024: 10 · 2023: 10 · 2022: 25 · 2021: 11 · 2020: 15 · 2019: 5 · 2018: 8 · 2017: 6 · 2016: 6 · 2015: 4 · 2014: 3
- FWCI
- 4.49
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Comisión Nacional de Investigación Científica y Tecnológica (1130216, R09I2001)
- Topics
- Health Sciences › Medicine › Hematology › Platelet Disorders and Treatments
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Antiplatelet Therapy and Cardiovascular Diseases
- Health Sciences › Medicine › Hematology › Blood Coagulation and Thrombosis Mechanisms
- Keywords
- chlorogenic acid, platelet activation, platelet aggregation, thrombus formation, antiplatelet activity, adenosine A2A receptor, adenylate cyclase, cAMP/PKA signaling, platelet-leukocyte interaction, soluble P-selectin, soluble CD40 ligand, RANTES, IL-1β, arterial thrombosis, bleeding time, aspirin
- MeSH terms
- Adenine, Cyclic AMP, Adenosine Triphosphate, Animals, Binding Sites, Bleeding Time, Cell Communication, Cell Line, Cell Survival, Chlorogenic Acid, Collagen, Enzyme Activation, Humans, Leukocytes, Models, Molecular, Platelet Adhesiveness, Platelet Aggregation, Thrombosis, Triazines, Triazoles, Platelet Activation, Cyclic AMP-Dependent Protein Kinases, Hemorheology, Inflammation Mediators, P-Selectin, Platelet Glycoprotein GPIIb-IIIa Complex, Receptor, Adenosine A2A, Mice
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