Effects of Escherichia coli on Mixotrophic Growth of Chlorella minutissima and Production of Biofuel Precursors
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Chlorella minutissima was co-cultured with Escherichia coli in airlift reactors under mixotrophic conditions (glucose, glycerol, and acetate substrates) to determine possible effects of bacterial contamination on algal biofuel production. It was hypothesized that E. coli would compete with C. minutissima for nutrients, displacing algal biomass. However, C. minutissima grew more rapidly and to higher densities in the presence of E. coli, suggesting a symbiotic relationship between the organisms. At an initial 1% substrate concentration, the co-culture produced 200-587% more algal biomass than the axenic C. minutissima cultures. Co-cultures grown on 1% substrate consumed 23-737% more of the available carbon substrate than the sum of substrate consumed by E. coli and C. minutissima alone. At 1% substrate, total lipid and starch productivity were elevated in co-cultures compared to axenic cultures indicating that bacterial contamination was not detrimental to the production of biofuel precursors in this specific case. Bio-fouling of the reactors observed in co-cultures and acid formation in all mixotrophic cultures, however, could present challenges for scale-up.
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At 1% substrate concentration, the total starch productivity as well as lipid productivity increased in all the co-cultures compared to axenic conditions.
“At 1% substrate concentration, the total starch productivity as well as lipid productivity increased in all the co-cultures compared to axenic conditions.”
From Toward the Enhancement of Microalgal Metabolite Production through Microalgae–Bacteria Consortia (2021) · cites this paper as [72]
Higgins and VanderGheynst reported significant increments in the starch produced by Chlorella minutissima when co-cultured with E. coli under mixotrophic conditions (glucose, glycerol, and acetate substrates).
“Higgins and VanderGheynst [72] reported significant increments in the starch produced by Chlorella minutissima when co-cultured with E. coli under mixotrophic conditions (glucose, glycerol, and acetate substrates).”
From Toward the Enhancement of Microalgal Metabolite Production through Microalgae–Bacteria Consortia (2021) · cites this paper as [72]
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- Authors
- Higgins, B. T., & VanderGheynst, J. S.
- Affiliations
- University of California, Davis (US)
- Corresponding author
- VanderGheynst, Jean S.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2014-05-07
- Volume
- 9
- Issue
- 5
- Pages
- e96807
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC4013066
- OpenAlex
- W2077651348
- Cited by
- 89
- References
- 56
- Citations by year
- 2026: 5 · 2025: 11 · 2024: 7 · 2023: 4 · 2022: 7 · 2021: 12 · 2020: 11 · 2019: 12 · 2018: 8 · 2017: 2 · 2016: 4 · 2015: 4 · 2014: 2
- FWCI
- 1.32
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- eScholarship (California Digital Library) (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- Figshare (submittedVersion, cc-by)
- Funders
- National Science Foundation (0948021, 1139644)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Keywords
- Chlorella minutissima, Escherichia coli, mixotrophic cultivation, algal-bacterial co-culture, biofuel precursors, lipid productivity, bacterial contamination, airlift bioreactor, glycerol, acetate, glucose, reactor fouling, acid production, symbiosis
- MeSH terms
- Carbon, Chlorella, Culture Media, Escherichia coli, Glucose, Humans, Starch, Biomass, Coculture Techniques, Biofuels
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