Increased pigment and lipid content, lipid variety, and cell and population size of the microalgae Chlorella spp. when co-immobilized in alginate beads with the microalgae-growth-promoting bacterium Azospirillum brasilense
Abstract
Three strains of the freshwater microalgae used for wastewater treatment, Chlorella vulgaris and Chlorella sorokiniana co-immobilized separately in alginate beads with the microalgae-growth-promoting bacterium Azospirillum brasilense Cd, resulted in significant changes in microalgal-population size, cell size, cell cytology, pigment, lipid content, and the variety of fatty acids produced in comparison with microalgae immobilized in alginate without the bacterium. Cells of C. vulgaris UTEX 2714 did not change in size, but the population size within the beads significantly increased. On the other hand, C. vulgaris UTEX 395 cells grew 62% larger, but their numbers did not increase. The population of C. sorokiniana UTEX 1602 increased, but not their cell size. The content of pigments chlorophyll a and b, lutein, and violoaxanthin increased in all microalgal species. The lipid content also significantly increased in all three strains, and the number of different fatty acids in the microalgae increased from four to eight. This study indicates that the microalgae-growth-promoting bacterium induced significant changes in the metabolism of the microalgae.
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In co-cultures of three Chlorella strains with A. brasilense immobilized in alginate beads, concentration and variety of fatty acids increased, reaching up to eight different fatty acids compared to four to five in microalgae-only immobilized cells.
“In all the co-cultures, the concentration and variety of fatty acids increased, reaching up to eight different fatty acids in microalgae co-immobilized with the MGPB in comparison to four to five in microalgae-only immobilized cells.”
From Toward the Enhancement of Microalgal Metabolite Production through Microalgae–Bacteria Consortia (2021) · cites this paper as [75]
A significant increase in these four pigments was observed in C. sorokiniana when co-immobilized in alginate beads with A. brasilense.
“Likewise, a significant increase in these four pigments was observed in the microalga C. sorokiniana when co-immobilized in alginate beads with A. brasilense [75].”
From Toward the Enhancement of Microalgal Metabolite Production through Microalgae–Bacteria Consortia (2021) · cites this paper as [75]
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- Authors
- de-Bashan, L. E., Bashan, Y., Moreno, M., Lebsky, V. K., & Bustillos, J. J.
- Venue
- Canadian Journal of Microbiology
- Publisher
- Canadian Science Publishing
- Published
- 2002-06-01
- Volume
- 48
- Issue
- 6
- Pages
- 514-521
- ISSN
- 0008-4166 (ISSN-L)
- 1480-3275
- OpenAlex
- W2159797801
- Cited by
- 222
- References
- 32
- Citations by year
- 2026: 2 · 2025: 7 · 2024: 8 · 2023: 8 · 2022: 7 · 2021: 17 · 2020: 17 · 2019: 20 · 2018: 13 · 2017: 16 · 2016: 16 · 2015: 16 · 2014: 12 · 2013: 13 · 2012: 12
- FWCI
- 3.25
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Canadian Journal of Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
- Keywords
- Chlorella vulgaris, Chlorella sorokiniana, Azospirillum brasilense, microalgae-bacteria co-culture, chlorophyll content, carotenoids, lipid content, fatty acid composition, wastewater treatment
- MeSH terms
- Alginates, Chlorella, Lipids, Microscopy, Electron, Pigments, Biological, Water Microbiology, Azospirillum brasilense, Waste Management, Cells, Immobilized, Lipid Metabolism
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