Cited by 238Machine Learning in BioinformaticsRNA and protein synthesis mechanismsFractal and DNA sequence analysis

Identification of Real MicroRNA Precursors with a Pseudo Structure Status Composition Approach

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Liu, Bin, Fang, Longyun, Liu, Fule, Wang, Xiaolong, Chen, Junjie, Chou, Kuo-Chen·2015·PLOS ONE

Abstract

Containing about 22 nucleotides, a micro RNA (abbreviated miRNA) is a small non-coding RNA molecule, functioning in transcriptional and post-transcriptional regulation of gene expression. The human genome may encode over 1000 miRNAs. Albeit poorly characterized, miRNAs are widely deemed as important regulators of biological processes. Aberrant expression of miRNAs has been observed in many cancers and other disease states, indicating they are deeply implicated with these diseases, particularly in carcinogenesis. Therefore, it is important for both basic research and miRNA-based therapy to discriminate the real pre-miRNAs from the false ones (such as hairpin sequences with similar stem-loops). Particularly, with the avalanche of RNA sequences generated in the postgenomic age, it is highly desired to develop computational sequence-based methods in this regard. Here two new predictors, called "iMcRNA-PseSSC" and "iMcRNA-ExPseSSC", were proposed for identifying the human pre-microRNAs by incorporating the global or long-range structure-order information using a way quite similar to the pseudo amino acid composition approach. Rigorous cross-validations on a much larger and more stringent newly constructed benchmark dataset showed that the two new predictors (accessible at http://bioinformatics.hitsz.edu.cn/iMcRNA/) outperformed or were highly comparable with the best existing predictors in this area.

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Authors
Liu, B., Fang, L., Liu, F., Wang, X., Chen, J., & Chou, K.-C.
Affiliations
  • Harbin Institute of Technology (CN)
  • King Abdulaziz University (SA)
Corresponding author
Liu, Bin
Venue
PLOS ONE
Published
2015-03-30
Volume
10
Issue
3
Pages
e0121501
ISSN
1932-6203 (ISSN-L)
OpenAlex
W2001168095
Cited by
238
References
93
Citations by year
2026: 1 · 2025: 4 · 2024: 1 · 2023: 3 · 2022: 6 · 2021: 9 · 2020: 19 · 2019: 29 · 2018: 46 · 2017: 26 · 2016: 48 · 2015: 46
FWCI
24.01
Type
journal article
Language
en
Open access
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Natural Science Foundation of China (HIT.NSRIF.2013103, 61272383, 61300112)
  • Harbin Institute of Technology
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Machine Learning in Bioinformatics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fractal and DNA sequence analysis
Keywords
pseudo amino acid composition, RNA secondary structure, human miRNAs, sequence-based prediction, benchmark dataset
MeSH terms
Amino Acids, Base Sequence, Humans, Nucleic Acid Conformation, Nucleotides, RNA Precursors, Genome, Human, Computational Biology, MicroRNAs, RNA, Small Untranslated

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