A Cellular Function for the RNA-Interference Enzyme Dicer in the Maturation of the let-7 Small Temporal RNA
Abstract
The 21-nucleotide small temporal RNA (stRNA) let-7 regulates developmental timing in Caenorhabditis elegans and probably in other bilateral animals. We present in vivo and in vitro evidence that in Drosophila melanogaster a developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce mature let-7 stRNA. Targeted destruction in cultured human cells of the messenger RNA encoding the enzyme Dicer, which acts in the RNA interference pathway, leads to accumulation of the let-7 precursor. Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression.
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The enzyme responsible for cleavage of miRNA hairpin precursors is Dicer.
“The enzyme responsible for this cleavage is Dicer (Hutvagner et al. 2001;Ketting et al. 2001).”
From A microRNA array reveals extensive regulation of microRNAs during brain development (2003) · cites this paper as Hutvagner et al. 2001
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- Authors
- Hutvágner, G., McLachlan, J., Pasquinelli, A. E., Bálint, E., Tuschl, T., & Zamore, P. D.
- Affiliations
- Department of Biochemistry and Molecular Pharmacology;
- Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, MA 02114, USA.
- Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA.
- Department of Cellular Biochemistry, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, D-37077 Göttingen, Germany.
- Venue
- Science
- Published
- 2001-08-03
- Volume
- 293
- Issue
- 5531
- Pages
- 834-838
- ISSN
- 0036-8075
- 1095-9203
- Cited by
- 2,287
- References
- 2
- Language
- en
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