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Detection of Cytosine Methylation in Ancient DNA from Five Native American Populations Using Bisulfite Sequencing
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Bisulfite conversion is also extremely harmful to DNA and, thus, generally not recommended for aDNA, despite a few successes on limited numbers of samples.
“However, bisulfite conversion is also extremely harmful to DNA and, thus, generally not recommended for aDNA, despite a few successes on limited numbers of samples 244,245 .”
From Ancient DNA analysis (2021) · cites this paper as [245]
The Discussion (page unavailable) says sodium bisulfite can reduce viable DNA molecules by 90% or more, supporting the claim that conversion is harmful to aDNA. But the paper reports successful methylation recovery in large numbers of ancient remains, and its Conclusions say bisulfite sequencing may be useful for well-preserved aDNA; it does not generally advise against the method or characterize its successes as limited to a few samples. (LLM, conf=0.88)
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Orlando, L., Allaby, R., Skoglund, P., Der Sarkissian, C., Stockhammer, P. W., Ávila-Arcos, M. C., Fu, Q., Krause, J., Willerslev, E., Stone, A. C., et al. (2021) · Nature Reviews Methods Primers · doi:10.1038/s43586-020-00011-0 · Cited by 411
Human evolution: a tale from ancient genomes
Llamas, B., Willerslev, E., & Orlando, L. (2017) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2015.0484 · Cited by 100
Assessing DNA sequence alignment methods for characterizing ancient genomes and methylomes
Poullet, M., & Orlando, L. (2020) · Frontiers in Ecology and Evolution · doi:10.3389/fevo.2020.00105 · Cited by 61
Metadata
- Authors
- Smith, R. W. A., Monroe, C., & Bolnick, D. A.
- Venue
- PLOS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2015-05-27
- Volume
- 10
- Issue
- 5
- Pages
- e0125344
- ISSN
- 1932-6203
- PMC
- PMC4445908
- Cited by
- 43
- References
- 2
- Language
- en
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