Screening of probiotic candidates in human oral bacteria for the prevention of dental disease
Open accessAbstract
The oral cavity in healthy subjects has a well-balanced microbiota that consists of more than 700 species. However, a disturbance of this balance, with an increase of harmful microbes and a decrease of beneficial microbes, causes oral disorders such as periodontal disease or dental caries. Nowadays, probiotics are expected to confer oral health benefits by modulating the oral microbiota. This study screened new probiotic candidates with potential oral health benefits and no harmful effects on the oral cavity. We screened 14 lactobacillus strains and 36 streptococcus strains out of 896 oral isolates derived from healthy subjects. These bacteria did not produce volatile sulfur compounds or water-insoluble glucan, had higher antibacterial activity against periodontal bacteria, and had higher adherence activity to oral epithelial cells or salivary-coated hydroxyapatite in vitro. We then evaluated the risk of primary cariogenicity and infective endocarditis of the selected oral isolates. As a result, Lactobacillus crispatus YIT 12319, Lactobacillus fermentum YIT 12320, Lactobacillus gasseri YIT 12321, and Streptococcus mitis YIT 12322 were selected because they showed no cariogenic potential in an artificial mouth system and a lower risk of experimental infective endocarditis in a rat model. These candidates are expected as new probiotics with potential oral health benefits and no adverse effects on general health.
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Probiotic therapy exerts a beneficial effect on dental caries, periodontitis, and pharyngitis in the oral cavity, as well as on obesity, hypertension, insulin resistance, and heart failure via the gut.
“Given, however, that probiotic therapy exerts a beneficial effect on dental caries, periodontitis, and pharyngitis in the oral cavity[408–411], as well as on obesity, hypertension, insulin resistance, and heart failure via the gut(reviewed:[399,403])”
From Enterosalivary nitrate metabolism and the microbiome: Intersection of microbial metabolism, nitric oxide and diet in cardiac and pulmonary vascular health (2017) · cites this paper as [410]
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- Authors
- Terai, T., Okumura, T., Imai, S., Nakao, M., Yamaji, K., Ito, M., Nagata, T., Kaneko, K., Miyazaki, K., Okada, A., Nomura, Y., & Hanada, N.
- Affiliations
- Yakult Central Institute (JP)
- Tsurumi University (JP)
- Corresponding author
- Terai, Tomohiko
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2015-06-08
- Volume
- 10
- Issue
- 6
- Pages
- e0128657
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC4459870
- OpenAlex
- W1561959808
- Cited by
- 99
- References
- 55
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 10 · 2023: 15 · 2022: 11 · 2021: 8 · 2020: 10 · 2019: 11 · 2018: 8 · 2017: 13 · 2016: 4 · 2015: 1
- FWCI
- 11.74
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Japan Society for the Promotion of Science (25463270, 15K20655)
- Topics
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Health Sciences › Dentistry › Periodontics › Dental Health and Care Utilization
- Keywords
- probiotics, oral microbiota, periodontal disease, dental caries, Lactobacillus, Streptococcus mitis, Lactobacillus crispatus, Lactobacillus fermentum, Lactobacillus gasseri, antibacterial activity, volatile sulfur compounds, water-insoluble glucan, infective endocarditis, oral health
- MeSH terms
- Adult, Aged, Animals, Anti-Bacterial Agents, Bacteria, Bacterial Adhesion, Cattle, Dental Caries, Dental Enamel, DNA, Ribosomal, Endocarditis, Epithelial Cells, Female, Glucans, Humans, Lactobacillus, Male, Microbial Sensitivity Tests, Middle Aged, Mouth, Stomatognathic Diseases, Oral Health, Solubility, Sucrose, Sulfur Compounds, Durapatite, Probiotics
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