Tiron Inhibits UVB-Induced AP-1 Binding Sites Transcriptional Activation on MMP-1 and MMP-3 Promoters by MAPK Signaling Pathway in Human Dermal Fibroblasts
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Recent research found that Tiron was an effective antioxidant that could act as the intracellular reactive oxygen species (ROS) scavenger or alleviate the acute toxic metal overload in vivo. In this study, we investigated the inhibitory effect of Tiron on matrix metalloproteinase (MMP)-1 and MMP-3 expression in human dermal fibroblast cells. Western blot and ELISA analysis revealed that Tiron inhibited ultraviolet B (UVB)-induced protein expression of MMP-1 and MMP-3. Real-time quantitative PCR confirmed that Tiron could inhibit UVB-induced mRNA expression of MMP-1 and MMP-3. Furthermore, Tiron significantly blocked UVB-induced activation of the MAPK signaling pathway and activator protein (AP)-1 in the downstream of this transduction pathway in fibroblasts. Through the AP-1 binding site mutation, it was found that Tiron could inhibit AP-1-induced upregulation of MMP-1 and MMP-3 expression through blocking AP-1 binding to the AP-1 binding sites in the MMP-1 and MMP-3 promoter region. In conclusion, Tiron may be a novel antioxidant for preventing and treating skin photoaging UV-induced.
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UV radiation activates MMPs and induces their expression in skin.
“Studies have shown that UV radiation activates MMPs and induces their expression in skin [38].”
From Boosting the Photoaged Skin: The Potential Role of Dietary Components (2021) · cites this paper as [38]
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- Authors
- Lu, J., Guo, J.-H., Tu, X.-L., Zhang, C., Zhao, M., Zhang, Q.-W., & Gao, F.-H.
- Affiliations
- Shanghai Jiao Tong University (CN)
- Henan University of Science and Technology (CN)
- Tongji University (CN)
- Zhengzhou University (CN)
- Corresponding author
- Zhang, Quan-Wu
- Gao, Feng-Hou
- Venue
- PLOS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2016-08-03
- Volume
- 11
- Issue
- 8
- Pages
- e0159998
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC4972414
- OpenAlex
- W2493242788
- Cited by
- 79
- References
- 47
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 9 · 2023: 13 · 2022: 6 · 2021: 9 · 2020: 4 · 2019: 12 · 2018: 7 · 2017: 10
- FWCI
- 11.62
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (81172322, 81172322, 81302006, 81302006)
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
- Keywords
- Tiron, UV-B radiation, MMP-1, matrix metalloproteinase-3, AP-1 transcription factor, MAPK signaling, dermal fibroblasts, photoaging, antioxidants, reactive oxygen species, promoter regulation
- MeSH terms
- Antioxidants, Binding Sites, Cells, Cultured, Fibroblasts, Humans, Promoter Regions, Genetic, 1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt, Ultraviolet Rays, Signal Transduction, Transcriptional Activation, Skin Aging, Transcription Factor AP-1, Matrix Metalloproteinase 3, Cytoprotection, Dermis, Matrix Metalloproteinase 1, MAP Kinase Signaling System
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