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Cited by 24Skin Protection and AgingNonmelanoma Skin Cancer StudiesDNA Repair Mechanisms

Transcriptome Analysis Identifies the Dysregulation of Ultraviolet Target Genes in Human Skin Cancers

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Shen, Yao, Kim, Arianna L., Du, Rong, Liu, Liang·2016·PLOS ONE
DOI 10.1371/journal.pone.0163054PMC PMC5028058
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Abstract

Exposure to ultraviolet radiation (UVR) is a major risk factor for both melanoma and non-melanoma skin cancers. In addition to its mutagenic effect, UVR can also induce substantial transcriptional instability in skin cells affecting thousands of genes, including many cancer genes, suggesting that transcriptional instability may be another important etiological factor in skin photocarcinogenesis. In this study, we performed detailed transcriptomic profiling studies to characterize the kinetic changes in global gene expression in human keratinocytes exposed to different UVR conditions. We identified a subset of UV-responsive genes as UV signature genes (UVSGs) based on 1) conserved UV-responsiveness of this subset of genes among different keratinocyte lines; and 2) UV-induced persistent changes in their mRNA levels long after exposure. Interestingly, 11 of the UVSGs were shown to be critical to skin cancer cell proliferation and survival. Through computational Gene Set Enrichment Analysis, we demonstrated that a significant portion of the UVSGs were dysregulated in human skin squamous cell carcinomas, but not in other human malignancies. This highlights the potential and specificity of the UVSGs in clinical diagnosis of UV damage and stratification of skin cancer risk.

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  • Ultraviolet radiation causes significant changes in the skin and modifies immunological responses.

    “Ultraviolet radiation has been shown to cause significant changes in the skin as well as modify immunological responses (Shen et al. 2016).”

    From Skin protection from solar ultraviolet radiation using natural compounds: a review (2024) · cites this paper as Shen et al. 2016

    The Results section (“Transcriptomic responses to different UVR conditions”; page unavailable) reports substantial UV-induced gene-expression changes in cultured human keratinocytes, including enrichment of inflammatory-response and leukocyte-activation pathways. This supports changes in skin cells and immune-related gene expression, but the Discussion cautions that cultured cells do not capture interactions among cell types in vivo; the paper does not directly establish a modified immunological response in skin. (LLM, conf=0.84)

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Cited by

Sorted by citations

Authors
Shen, Y., Kim, A. L., Du, R., & Liu, L.
Affiliations
Columbia University (US)
Corresponding author
Liu, Liang
ORCID
Venue
PLOS ONE
Publisher
Public Library of Science (PLoS)
Published
2016-09-19
Volume
11
Issue
9
Pages
e0163054
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0163054
PMC
PMC5028058
OpenAlex
W2521246436
Cited by
24
References
5
Citations by year
2024: 2 · 2023: 3 · 2022: 5 · 2021: 3 · 2020: 1 · 2019: 3 · 2018: 5 · 2017: 2
FWCI
4.01
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
  • Columbia Academic Commons (Columbia University)
Funders
  • Office of Extramural Research, National Institutes of Health (K01AR064315, P30 ES009089)
  • American Skin Association
  • National Institute of Arthritis and Musculoskeletal and Skin Diseases (P30AR44535)
Topics
  • Health Sciences › Medicine › Dermatology › Skin Protection and Aging
  • Health Sciences › Medicine › Epidemiology › Nonmelanoma Skin Cancer Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
Keywords
ultraviolet radiation, skin cancer, keratinocytes, squamous cell carcinoma, melanoma, photocarcinogenesis, gene expression profiling
MeSH terms
Carcinoma, Squamous Cell, Dose-Response Relationship, Radiation, Humans, Skin Neoplasms, Ultraviolet Rays, Gene Expression Profiling, Cell Proliferation, Transcriptome

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