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A multi-chamber microfluidic intestinal barrier model using Caco-2 cells for drug transport studies

Tan, Hsih-Yin, Trier, Sofie, Rahbek, Ulrik L., Dufva, Martin, Kutter, Jörg P., Andresen, Thomas L.·2018·PLOS ONE
DOI 10.1371/journal.pone.0197101PMC PMC5944968
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  • Gut epithelial cells require physiologically relevant fluid shear stress (FSS) from the perfusion flow to express their organ cell functions.

    “Practically gut epithelial cells require physiological relevant fluid shear stress (FSS) from the perfusion flow, to express their organ cell functions.14,15”

    From Gut-liver-axis microphysiological system for studying cellular fluidic shear stress and inter-tissue interaction (2022) · cites this paper as [15]

    In Results and discussion (page not provided), the paper reports that physiologically relevant perfusion shear appeared to induce Mucin-2 production in Caco-2 cells, unlike static Transwell cultures. That supports a role for flow in expressing one intestinal function, but not the broader claim that gut epithelial cells require it to express their organ functions generally; the Introduction also says Caco-2 cells differentiate and form tight monolayers in static Transwells. (LLM, conf=0.86)

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Cited by

Sorted by citations

Authors
Tan, H.-Y., Trier, S., Rahbek, U. L., Dufva, M., Kutter, J. P., & Andresen, T. L.
ORCID
Dufva, Martin
Venue
PLOS ONE
Publisher
Public Library of Science (PLoS)
Published
2018-05-10
Volume
13
Issue
5
Pages
e0197101
ISSN
1932-6203
DOI
10.1371/journal.pone.0197101
PMC
PMC5944968
Cited by
133
References
7
Language
en
Licence
creativecommons.org/licenses/by/4.0/ (vor)
Funders
  • Lundbeckfonden
  • Teknologi og Produktion, Det Frie Forskningsråd

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