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The effect of aging in primary human dermal fibroblasts

Lago, Juliana Carvalhães, Puzzi, Maria Beatriz·2019·PLOS ONE

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Crossref · 10.1371/journal.pone.0219165

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Fig 1 Images from premature senescence activity induced by stress. 1a –Primary HDF from the young donors control assay at passage 5. The absence of the blue color indicates cells with non-senescence activity. 1b –Primary HDF from elderly donors at passage 5. The blue color indicates a few senescent cells. 1c –Primary HDF from young donors submitted to UVB irradiation at passage 5. The blue color indicates cells with senescence activity. 1d –Primary HDF from young donors submitted to accelerated proliferation at passage 20. The blue color indicates cells with senescence activity.
Fig 1 Images from premature senescence activity induced by stress. 1a –Primary HDF from the young donors control assay at passage 5. The absence of the blue color indicates cells with non-senescence activity. 1b –Primary HDF from elderly donors at passage 5. The blue color indicates a few senescent cells. 1c –Primary HDF from young donors submitted to UVB irradiation at passage 5. The blue color indicates cells with senescence activity. 1d –Primary HDF from young donors submitted to accelerated proliferation at passage 20. The blue color indicates cells with senescence activity.CC BY · PubMed Central
Fig 2 Cell growth rate. The HDF cell growth rates from all the in-vitro aging models were analyzed after 3 and 6 days of incubation. Data is expressed in number of cells, and the results were compared between days 0, 3, and 6 (*) and between young HDF donors versus aged cells (**). Test Z statistics (p<0.0001; ns: not significant).
Fig 2 Cell growth rate. The HDF cell growth rates from all the in-vitro aging models were analyzed after 3 and 6 days of incubation. Data is expressed in number of cells, and the results were compared between days 0, 3, and 6 (*) and between young HDF donors versus aged cells (**). Test Z statistics (p<0.0001; ns: not significant).CC BY · PubMed Central
Fig 3 Cell growth rate: Real-time evaluation by resistance and impedance measurement with ECIS (electric cell-substrate impedance sensing). The analysis of the cell growth rate, evaluated in real time by determining the resistance in relation to time, obtained via impedance sensing indicates an increase in impedance in comparison to young control.
Fig 3 Cell growth rate: Real-time evaluation by resistance and impedance measurement with ECIS (electric cell-substrate impedance sensing). The analysis of the cell growth rate, evaluated in real time by determining the resistance in relation to time, obtained via impedance sensing indicates an increase in impedance in comparison to young control.CC BY · PubMed Central

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Research works printed but not linked: 10

  1. ED Lephart, 2018, A review of the role of estrogen in dermal aging and facial attractiveness in women, 10.1111/jocd.12508 · DOI · Paper page
  2. A Ibuki, 2018, Aging-like physiological changes in the skin of Japanese obese diabetic patients, Vol. 6
  3. S Hatem, 2018, Recent advances in antioxidant cosmeceutical topical delivery, 10.2174/1567201815666180214143551 · DOI
  4. W Kaisers, 2017, Age, gender and UV-exposition related effects on gene expression in in vivo aged short term cultivated human dermal fibroblasts, Vol. 12, e0175657
  5. H Chen, 2017, AKT and its related molecular feature in aged mice skin, Vol. 12, e0178969
  6. T Shoko, 2018, Anti-aging potential of extracts from Sclerocarya birrea (A. Rich.) Hochst and its chemical profiling by UPLC-Q-TOF-MS, Vol. 18, 54
  7. A Porcheron, 2013, Aspects of facial contrast decrease with age and are cues for age perception, Vol. 8, e57985
  8. H Abbas, 2018, Dermal anti-oxidant, anti-inflammatory and anti-aging effects of Compritol ATO-based Resveratrol colloidal carriers prepared using mixed surfactants, 10.1016/j.ijpharm.2018.01.054 · DOI
  9. L Subedi, 2017, Resveratrol-Enriched Rice Attenuates UVB-ROS-Induced Skin Aging via Downregulation of Inflammatory Cascades, Vol. 2017
  10. V Kostyuk, 2012, Photo-oxidation products of skin surface squalene mediate metabolic and inflammatory responses to solar UV in human keratinocytes, Vol. 7, e44472

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Metadata

Authors
Lago, J. C., & Puzzi, M. B.
Venue
PLOS ONE
Published
2019-07-03
Volume
14
Issue
7
Pages
e0219165
ISSN
1932-6203
Cited by
168
References
5
Language
en

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