Cited by 90Aquaculture Nutrition and GrowthAquaculture disease management and microbiotaAnimal Nutrition and Physiology

Effects of dietary supplementation with a microalga (Schizochytrium sp.) on the hemato-immunological, and intestinal histological parameters and gut microbiota of Nile tilapia in net cages

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Souza, Felipe Pinheiro de, Lima, Ed Christian Suzuki de, Urrea-Rojas, Angela Maria, Suphoronski, Suelen Aparecida, Facimoto, César Toshio, Bezerra Júnior, Jailton da Silva, Oliveira, Thalita Evani Silva de, Pereira, Ulisses de Pádua +3 more·2020·PLoS ONE

Abstract

Nutritional improvements in intensive aquaculture production systems is necessary for the reduction of stress, maximum utilization of nutritional components, and expression of the genetic potential of fish. The objective of this study was to evaluate the hemato-immunological, and histological parameters and gut microbiota of Nile tilapia fed with the microalga Schizochytrium sp. Males of Nile tilapia were distributed among eight net cages (6 m3), and fed for 105 days with two diets: control (CON), without Schizochytrium sp., and supplemented (SUP), with 1.2% Schizochytrium sp. in the diet. The final weight, mortality, hematocrit, total erythrocyte count (RBC), hemoglobin, hematimetric indices, white blood cell count (WBC), total protein, and serum lysozyme were measured. Alterations in intestinal morphology were evaluated. The gut microbiota was evaluated with next-generation sequencing. No significant differences (p>0.05) were found in the final weight and mortality between diets. Regarding the hematological parameters, a difference (p<0.05) was detected only in RBC, with there being lower values in the SUP, although this group also showed a tendency toward having an increased mean corpuscular hemoglobin level. There were no differences (p>0.05) in total protein and serum lysozyme concentrations or in WBCs between diets, except for lymphocytes, which presented lower values (p<0.05) in the SUP, suggesting immunomodulation by the polyunsaturated fatty acids present in the microalga. There was no difference (p>0.05) in the intestinal morphology between diets. Metagenomic data indicated greater richness (represented by the Chao index) and a higher abundance of the bacterial phylum Firmicutes in the gut microbiota of the tilapia fed with the SUP diet, demonstrating that the digestion and use of the components of the microalga could influence the microbial community. The results indicated that the microalga had modulatory effects on blood cells and the intestinal microbiota, without affecting the structure and integrity of the intestinal villi.

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  • The addition of a 1.2% Schizochytrium sp. meal to the diet influenced the gut microbiota, resulting in improved Nile tilapia health.

    “The addition of a 1.2% Schizochytrium sp.meal to the diet influenced the gut microbiota, resulting in improved Nile tilapia health (Souza et al., 2020).”

    From Potential of microalgae as a sustainable feed ingredient for aquaculture (2021) · cites this paper as Souza et al., 2020

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Research works printed but not linked: 4

  1. [1]FAO. The State of World Fisheries and Aquaculture 2018—Meeting the sustainable development goals [Internet]. Vol. 35. 2018. 176 p. ftp://ftp.fao.org/docrep/fao/011/i0250e/i0250e.pdf
  2. [14]dos Santos SKA, Guilherme de Souza Moura MMP, Aline Danielle Souza Prates ALF, Azevedo RC. Microalga Schizochytrium sp. em Rações para Tilápia do Nilo. Cad Ciências Agrárias. 2015; 7(1): 75– 9.
  3. [19]Kumala FB, Wahjuningrum D, Setiawati M. Effects of dietary algae, fungi and herb on the growth and innate immunity of Nile tilapia Oreochromis niloticus challenged with Streptococcus agalactiae. AACL Bioflux. 2018; 11(4): 1368– 77.
  4. [42]Araujo DDM, Pezzato AC, Barros MM. Hematology of Nile tilapia fed diets with vegetable oils and stimulated by cold. Pesqui Agropecu Bras. 2011; 46(3): 294– 302.

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Authors
Souza, F. P. D., Lima, E. C. S. D., Urrea-Rojas, A. M., Suphoronski, S. A., Facimoto, C. T., Bezerra Júnior, J. D. S., Oliveira, T. E. S. D., Pereira, U. D. P., Santis, G. W. D., Oliveira, C. A. L. D., & Lopera-Barrero, N. M.
Affiliations
Universidade Estadual de Londrina (BR)
Venue
PLoS ONE
Published
2020-01-02
Volume
15
Issue
1
Pages
e0226977
ISSN
1932-6203 (ISSN-L)
OpenAlex
W2996763297
Cited by
90
References
72
Citations by year
2026: 10 · 2025: 12 · 2024: 17 · 2023: 15 · 2022: 17 · 2021: 12 · 2020: 6 · 2018: 1
FWCI
9.03
Type
journal article
Language
en
Open access
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
Funders
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico
Topics
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
  • Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
Keywords
Schizochytrium, Oreochromis niloticus, fish immunomodulation, hematological parameters, serum lysozyme, white blood cell count, intestinal histology, gut microbiota, Firmicutes, polyunsaturated fatty acids, net cage aquaculture, fish nutrition
MeSH terms
Gastrointestinal Microbiome, Animals, Blood Cells, Body Weight, Intestines, Male, Dietary Supplements, Cichlids, Microalgae, Microbiota

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