The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Hydroxytyrosol Disposition in Humans
Abstract
Background: Animal and in vitro studies suggest that phenolic compounds in virgin olive oil are effective antioxidants. In animal and in vitro studies, hydroxytyrosol and its metabolites have been shown to be strong antioxidants. One of the prerequisites to assess their in vivo physiologic significance is to determine their presence in human plasma. Methods: We developed an analytical method for both hydroxytyrosol and 3-O-methyl-hydroxytyrosol in plasma. The administered dose of phenolic compounds was estimated from methanolic extracts of virgin olive oil after subjecting them to different hydrolytic treatments. Plasma and urine samples were collected from 0 to 12 h before and after 25 mL of virgin olive oil intake, a dose close to that used as daily intake in Mediterranean countries. Samples were analyzed by capillary gas chromatography–mass spectrometry before and after being subjected to acidic and enzymatic hydrolytic treatments. Results: Calibration curves were linear (r >0.99). Analytical recoveries were 42–60%. Limits of quantification were <1.5 mg/L. Plasma hydroxytyrosol and 3-O-methyl-hydroxytyrosol increased as a response to virgin olive oil administration, reaching maximum concentrations at 32 and 53 min, respectively (P <0.001 for quadratic trend). The estimated hydroxytyrosol elimination half-life was 2.43 h. Free forms of these phenolic compounds were not detected in plasma samples. Conclusions: The proposed analytical method permits quantification of hydroxytyrosol and 3-O-methyl-hydroxytyrosol in plasma after real-life doses of virgin olive oil. From our results, ∼98% of hydroxytyrosol appears to be present in plasma and urine in conjugated forms, mainly glucuronoconjugates, suggesting extensive first-pass intestinal/hepatic metabolism of the ingested hydroxytyrosol.
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Cited by
Dietary polyphenols and their biological significance
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“It h as been reported that around 98% of hydroxytyrosol is present in plasma and urine in conjugated forms, mainly glucuronoconjugates, suggesting an extensive first pass intestinal/ hepatic metabolism of the ingested primary forms [197-199] and the 3O-glucuronide of hydroxytyrosol shows stronger activ ity as a radica…”
BackgroundIbuprofen-like activity in extra-virgin olive oil
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Iron-chelation properties of phenolic acids bearing catechol and galloyl groups
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Valuable Nutrients and Functional Bioactives in Different Parts of Olive (Olea europaea L.)—A Review
Ghanbari, R., Anwar, F., Alkharfy, K. M., Gilani, A.-H., & Saari, N. (2012) · International Journal of Molecular Sciences · doi:10.3390/ijms13033291 · Cited by 600
Metadata
- Authors
- Miro-Casas, E., Covas, M.-I., Farre, M., Fito, M., Ortuño, J., Weinbrenner, T., Roset, P., & de la Torre, R.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 2003-06-01
- Volume
- 49
- Issue
- 6
- Pages
- 945-952
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W2084023771
- Cited by
- 295
- References
- 27
- FWCI
- 9.86
- Type
- journal article
- Language
- en
- Open access
- closed
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