CoChat
Sign Up
Cited by 125Clinical Laboratory Practices and Quality ControlDiabetes Management and ResearchAnalytical chemistry methods development

The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.

Stabilization of Glucose in Blood Samples: Why It Matters

Open access
Bruns, David E, Knowler, William C·2009·Clinical Chemistry
DOI 10.1373/clinchem.2009.126037PMC PMC2956743
Open this paper in CoChat — free account

Abstract

In this issue of Clinical Chemistry , Gambino et al. (1) describe careful studies of a blood-collection container that stabilizes the concentration of glucose in blood samples. At a time of an increasing disease burden attributable to diabetes and the use of lower glucose concentrations than in the past for the diagnosis of diabetes, this study and its findings are especially important. Measurements of glucose are used worldwide to diagnose diabetes and to identify patients at risk of developing diabetes [e.g., (2)(3)]. For both diagnosis and risk assessment, fixed cutpoints of plasma glucose concentrations are used to classify patients and to make decisions regarding management. For this reason, all steps in the analytical process require careful attention. Clinical chemists in hospital laboratories and diagnostic companies have made great strides in improving the measurement of glucose. With the use of enzymatic methods and sophisticated analyzers with stable optics, electronics, fluid handling, and other components, central clinical laboratories routinely achieve an astoundingly low within-laboratory imprecision (CV) of 1%–2%. [Glucose meters do not fare so well and are not recommended for diagnosis of diabetes (4).] By contrast, the preanalytical issues surrounding glucose measurements have not been solved. The loss of glucose in blood samples has been studied for many years (5). Glucose is lost through glycolysis at a rate of 5%–7%/h at concentrations near the reference interval. In absolute terms, a loss in glucose of about 0.67 mmol/L (12 mg/dL) occurs at a concentration of 5.55 mmol/L (100 mg/dL) after 2 h at room temperature (6). Higher rates of loss occur commonly, such as with increased ambient temperature and in samples with high white blood cell counts. In 1923, Major introduced potassium fluoride as a potent inhibitor of glycolysis (7), and fluoride, usually as NaF, has been used for decades to …

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1373/clinchem.2009.126037

Cites

11 of 11 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 11 references

Research works printed but not linked: 2

  1. 1923, JAMA, 81, 1952
  2. 2008, Surveys, 18

Cited by

Sorted by citations

Items 101–125 of 125

Related

See all

Metadata

Authors
Bruns, D. E., & Knowler, W. C.
Affiliations
  • University of Virginia (US)
  • National Institute of Diabetes and Digestive and Kidney Diseases (US)
ORCID
Knowler, William C
Venue
Clinical Chemistry
Publisher
Oxford University Press (OUP)
Published
2009-05-01
Volume
55
Issue
5
Pages
850-852
ISSN
  • 0009-9147 (ISSN-L)
  • 1530-8561
DOI
10.1373/clinchem.2009.126037
PMC
PMC2956743
OpenAlex
W2111541302
Semantic Scholar
3ca817c8f2f1deef73d71009f4d66a5cb410827f
Cited by
125
References
11
Citations by year
2026: 1 · 2025: 10 · 2024: 4 · 2023: 5 · 2022: 3 · 2021: 8 · 2020: 7 · 2019: 4 · 2018: 8 · 2017: 7 · 2016: 9 · 2015: 10 · 2014: 8 · 2013: 11 · 2012: 8
Type
journal article
Language
en
Open access
Licence
academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
Locations
  • Clinical Chemistry (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Institute of Diabetes and Digestive and Kidney Diseases
Topics
  • Health Sciences › Health Professions › Medical Laboratory Technology › Clinical Laboratory Practices and Quality Control
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Management and Research
  • Physical Sciences › Chemistry › Analytical Chemistry › Analytical chemistry methods development
Keywords
blood glucose stabilization, pre-analytical variables, glycolysis, potassium fluoride, sodium fluoride, diabetes diagnosis, pre-analytical errors
MeSH terms
Blood Glucose, Diabetes Mellitus, Humans, Specimen Handling, Reproducibility of Results

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review