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Androgen Receptor Regulates Expression of Skeletal Muscle–Specific Proteins and Muscle Cell Types
Abstract
C2C12 myoblasts expressing the androgen receptor (AR) were used to analyze the role of androgen-AR signaling pathway in skeletal muscle development. Marked up-regulation of AR expression was observed in differentiated myotubes. A nuclear run-on transcription assay demonstrated that transcription of the AR gene is increased during skeletal muscle cell differentiation. Regulation of skeletal muscle-specific protein expression by the androgen-AR signaling pathway was further analyzed using quadriceps skeletal muscle from wild-type (WT) and AR knock-out (ARKO) male mice. A histological analysis of quadriceps skeletal muscle indicates no morphological differences between ARKO and WT mice. However, the androgen-AR signaling pathway increases expression of slow-twitch-specific skeletal muscle proteins and downregulates fast-twitch-specific skeletal muscle proteins, resulting in an increase of slow-twitch muscle fiber type cells in quadriceps muscle.
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Analysis of mRNA reveals an upregulation of genes encoding slow-twitch muscle contractile proteins in AR-null mice.
“Analysis of mRNA reveals an upregulation of genes encoding slow-twitch muscle contractile proteins in AR-null mice (2, 59).”
From Sex-Based Differences in Skeletal Muscle Kinetics and Fiber-Type Composition (2015) · cites this paper as [2]
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Metadata
- Authors
- Altuwaijri, S., Lee, D. K., Chuang, K.-H., Ting, H.-J., Yang, Z., Xu, Q., Tsai, M.-Y., Yeh, S., Hanchett, L. A., Chang, H.-C., & Chang, C.
- Affiliations
- University of Rochester Medicine (US)
- Chang Gung University (TW)
- National Taiwan University (TW)
- Corresponding author
- Chang, Chawnshang
- ORCID
- Venue
- Endocrine
- Published
- 2004
- Volume
- 25
- Issue
- 1
- Pages
- 27-32
- ISSN
- 0969-711X (ISSN-L)
- OpenAlex
- W2056602633
- Cited by
- 58
- References
- 25
- Citations by year
- 2025: 2 · 2024: 4 · 2023: 5 · 2022: 3 · 2021: 1 · 2020: 1 · 2019: 4 · 2018: 4 · 2017: 4 · 2016: 4 · 2015: 5 · 2014: 2 · 2013: 1 · 2012: 1
- FWCI
- 0.36
- Type
- journal article
- Language
- en
- Update notices
- correction (2020-07-02)
- Open access
- closed
- Locations
- Endocrine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Diabetes and Digestive and Kidney Diseases (DK 60948, R01DK060948)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Keywords
- androgen receptor, androgen signaling, skeletal muscle development, muscle fiber type, slow-twitch muscle, fast-twitch muscle, myogenesis, C2C12 myoblasts, quadriceps
- MeSH terms
- Animals, Cell Differentiation, Cell Line, Hindlimb, Male, Mice, Inbred Strains, Muscle Proteins, Receptors, Androgen, Signal Transduction, Down-Regulation, Up-Regulation, Mice, Knockout, Muscle, Skeletal, Muscle Fibers, Skeletal, Muscle Fibers, Fast-Twitch, Muscle Fibers, Slow-Twitch, Myoblasts, Mice
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