Immunohistochemical study of cytochrome b5 in human adrenal gland and in adrenocortical adenomas from patients with Cushing’s syndrome
Open accessAbstract
Cytochrome b5, a component of the electron transfer system increases the relative activity of 17,20-lyase to 17alpha-hydroxylase of P450c17 in vitro. In the present study, immunohistochemical analysis of cytochrome b5 was performed in the human adrenal gland and in adrenocortical adenomas from patients with Cushing's syndrome. In the human adrenal gland, cytochrome b5 was stained in all three adrenocortical layers but the staining was most remarkable in the zona reticularis. All of the adenomas were composed mainly of compact cells, which exhibited immunoreactive staining for cytochrome b5 as well as for P450c17 and 3beta-hydroxysteroid dehydrogenase (3beta-HSD). The distribution of b5 in the adenomas was correlated with that of P450c17 rather than with that of 3beta-HSD. The immunoreactive staining for cytochrome b5 appeared to be more prominent in the two adenomas that produced relatively high concentrations of adrenal androgens than in adenomas that produced low concentrations of adrenal androgens. These results immunohistochemically support the functional association of b5 with androgen production through interaction with P450c17 and the previous finding that higher concentrations of cytochrome b5 are associated with greater production of adrenal androgens in adrenocortical adenomas from patients with Cushing's syndrome.
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Self-citation
- Authors
- YANASE, T., SASANO, H., YUBISUI, T., SAKAI, Y., TAKAYANAGI, R., & NAWATA, H.
- Affiliations
- Kyushu University (JP)
- Tohoku University (JP)
- University of Kochi (JP)
- Venue
- Endocrine Journal
- Publisher
- Japan Endocrine Society
- Published
- 1998
- Volume
- 45
- Issue
- 1
- Pages
- 89-95
- ISSN
- 0918-8959 (ISSN-L)
- 1348-4540
- OpenAlex
- W2069032561
- Semantic Scholar
- fe6fc62106c6336d34755bf48d9ef1ff9e55b2b6
- Cited by
- 54
- References
- 23
- Citations by year
- 2025: 1 · 2021: 3 · 2018: 1 · 2016: 1 · 2015: 1 · 2012: 3
- FWCI
- 4.30
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Endocrine Journal (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Adrenal and Paraganglionic Tumors
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal Regulation and Hypertension
- Keywords
- cytochrome b5, Cushing's syndrome, adrenal adenoma, adrenal gland, P450c17, 3β-hydroxysteroid dehydrogenase, adrenal androgen production, zona reticularis, 17,20-lyase, 17α-hydroxylase, steroidogenesis
- MeSH terms
- Adenoma, Adrenal Cortex, Adrenal Cortex Neoplasms, Cushing Syndrome, Humans, Immunohistochemistry, Male, Middle Aged, Steroid 17-alpha-Hydroxylase, Tissue Distribution, 3-Hydroxysteroid Dehydrogenases, Zona Reticularis, Cytochromes b5
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