Cited by 109Adrenal Hormones and DisordersHormonal Regulation and HypertensionSexual Differentiation and Disorders

Temporal and spatial distribution of corticosteroidogenic enzymes immunoreactivity in developing human adrenal

Narasaka, Toshiaki, Suzuki, Takashi, Moriya, Takuya, Sasano, Hironobu·2001·Molecular and Cellular Endocrinology

Abstract

Steroid hormones secreted by fetal adrenocortical cells are considered to be a requirement for a fetus to maintain intrauterine life, but, to date, the regulation of steroid hormone secretion has not been studied in detail in the human fetal adrenal gland. In this study, we examined the immunolocalization of steroidogenic enzymes and their local regulation, including adrenal 4-binding protein (Ad4BP or NR5A1), steroidogenic acute regulatory protein (StAR), P450 cholesterol side-chain cleavage (P450scc or CYP11A1), P450 17alpha-hydroxylase/17,20-lyase (P450c17 or CYP17), 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD), P450 21 hydroxylase (P450c21 or CYP21), dehydroepiandrosterone sulfotransferase (DHEA-ST), P450 oxidoreductase and cytochrome b5, in the human fetal adrenal gland (n=31) obtained from fetuses ranging in ages from 14 to 40 weeks of gestation. Ad4BP immunoreactivity was detected in all adrenocortical zones throughout gestation, suggesting that this nuclear protein is likely to be essential in the development of the human adrenal. Immunoreactivity for StAR, P450scc, P450c21, P450 oxidoreductase and cytochrome b5 was detected only in fetal and transitional zone between 14 and 22 weeks of gestation, but was detected in all three zones after 23 gestational weeks. 3beta-HSD immunoreactivity was not detected in any of the three cortical zones prior to 22 weeks of gestation, but became discernible in the transitional zone and definitive zone after 23 weeks. Immunoreactivity for P450c17 and DHEA-ST was detected in the transitional and fetal zones throughout gestation, but not in the definitive zone. These results suggest that the human adrenal cortex may produce dehydroepiandrosterone (DHEA) in the transitional and fetal zones throughout gestation, and cortisol in the transitional zone after the 23rd week of gestation.

Integrity signals

Registry

Crossref · 10.1016/s0303-7207(00)00445-7

Cites

48 of 48 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

3 of the 51 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Authors
Narasaka, T., Suzuki, T., Moriya, T., & Sasano, H.
Affiliations
Tohoku University (JP)
Corresponding author
Narasaka, Toshiaki
Venue
Molecular and Cellular Endocrinology
Publisher
Elsevier BV
Published
2001-03
Volume
174
Issue
1-2
Pages
111-120
ISSN
0303-7207 (ISSN-L)
OpenAlex
W1980898029
Cited by
109
References
51
Citations by year
2026: 1 · 2025: 3 · 2024: 1 · 2023: 3 · 2022: 4 · 2021: 8 · 2020: 4 · 2019: 3 · 2018: 6 · 2017: 4 · 2016: 7 · 2014: 2 · 2012: 2
FWCI
5.99
Type
journal article
Language
en
Open access
closed
Locations
  • Molecular and Cellular Endocrinology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Ministry of Education, Culture, Sports, Science and Technology (B-11470047)
  • Naito Foundation
  • Ministry of Health, Labour and Welfare (A-11137301)
  • Suzuken Memorial Foundation
  • Japan Society for the Promotion of Science
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal Regulation and Hypertension
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sexual Differentiation and Disorders
Keywords
fetal adrenal gland, adrenal cortex development, steroidogenic enzymes, immunoreactivity, gestational age, dehydroepiandrosterone, cortisol, steroidogenic acute regulatory protein, 3β-hydroxysteroid dehydrogenase, 17α-hydroxylase/17,20-lyase, 21-hydroxylase, cytochrome P450 oxidoreductase, cytochrome b5
MeSH terms
Steroidogenic Acute Regulatory Protein, Adrenal Cortex Hormones, Adrenal Glands, DNA-Binding Proteins, Enzymes, Female, Fetus, Humans, Immunohistochemistry, Male, Phosphoproteins, Pregnancy, Pregnancy Trimester, Second, Transcription Factors, Receptors, Cytoplasmic and Nuclear, Homeodomain Proteins, Fushi Tarazu Transcription Factors, Steroidogenic Factor 1

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review