Establishment of detailed reference values for luteinizing hormone, follicle stimulating hormone, estradiol, and progesterone during different phases of the menstrual cycle on the Abbott ARCHITECT® analyzer
Abstract
During a normal menstrual cycle, serum levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), estradiol, and progesterone can vary widely between cycles for the same woman, as well as between different woman. Reliable reference values based on the local population are important for correct interpretation of laboratory results. The purpose of our study was to determine detailed reference values for these hormones throughout the menstrual cycle using the Abbott ARCHITECT system. From 20 volunteers (age 20–36years) with normal cycles and no use of oral contraceptives, samples were taken every day during their cycle. Volunteers received three vaginal ultrasound examinations (days 10 and 13, and 1 or 2days after ovulation) to measure follicular and corpus luteum development. Hormone levels were measured using the corresponding ARCHITECT assay and were synchronized to the LH peak. Median, and 5th and 95th percentile values were determined for each day of the cycle, as well as for early follicular (days −15 to −6), late follicular (days −5 to −1), LH peak (day 0), early luteal (+1 to +4), mid-luteal (days +5 to +9), and late luteal (days +10 to +14) phases of the cycle. Based on our data, we were able to establish detailed reference values for LH, FSH, estradiol, and progesterone, which should aid in the interpretation of results for these reproductive hormones in a variety of circumstances. Clin Chem Lab Med 2006;44:883–7.
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Stricker et al. summarized mean, median, 5th, and 95th percentiles of daily levels of LH, FSH, E2, and P4 but did not report inhibin levels.
“In another study, Stricker et al. [54] summarized the mean, median, 5th, and 95th percentiles of the daily levels of LH, FSH, E . 2, and P . 4 of study participants but did not report inhibin levels.”
From A Survey of Mathematical Modeling of Hormonal Contraception and the Menstrual Cycle (2024) · cites this paper as [54]
Circulating levels of estrogens vary greatly during the course of the menstrual cycle and decrease significantly in postmenopausal women.
“Circulating levels of estrogens vary greatly during the course of the menstrual cycle and decrease significantly in postmenopausal women (Table 3) [[306], [307], [308]].”
From Human steroid biosynthesis, metabolism and excretion are differentially reflected by serum and urine steroid metabolomes: a comprehensive review (2019) · cites this paper as [306]
Population-based RIs based on monthly variation are available for hormones, but population-based RIs based on seasonal data are not common in routine practice.
“In clinical practice, population-based RIs (popRI) based on monthly variation is available for hormones [ 169 , 170 , 171 ] but unfortunately, the popRI based on seasonal data are not common, particularly in routine practice.”
From Physiological Rhythms and Biological Variation of Biomolecules: The Road to Personalized Laboratory Medicine (2023) · cites this paper as [171]
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- Authors
- Stricker, R., Eberhart, R., Chevailler, M.-C., Quinn, F. A., Bischof, P., & Stricker, R.
- Affiliations
- University of Geneva (CH)
- Venue
- Clinical Chemistry and Laboratory Medicine (CCLM)
- Publisher
- Walter de Gruyter GmbH
- Published
- 2006-01-01
- Volume
- 44
- Issue
- 7
- Pages
- 883-7
- ISSN
- 1434-6621 (ISSN-L)
- 1437-4331
- OpenAlex
- W2054292719
- Cited by
- 392
- References
- 9
- Citations by year
- 2026: 29 · 2025: 42 · 2024: 32 · 2023: 25 · 2022: 27 · 2021: 30 · 2020: 25 · 2019: 26 · 2018: 15 · 2017: 16 · 2016: 13 · 2015: 28 · 2014: 24 · 2013: 22 · 2012: 18
- FWCI
- 1.99
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Clinical Chemistry and Laboratory Medicine (CCLM) (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
- Health Sciences › Medicine › Reproductive Medicine › Ovarian function and disorders
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Keywords
- luteinizing hormone, follicle-stimulating hormone, estradiol, progesterone, menstrual cycle, reference values, reproductive hormones, follicular phase, luteal phase, ovulation, folliculogenesis, corpus luteum
- MeSH terms
- Adult, Estradiol, Female, Follicle Stimulating Hormone, Hematologic Tests, Humans, Luteinizing Hormone, Menstrual Cycle, Progesterone, Reference Values
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