Correlation of cortisol in 1-cm hair segment with salivary cortisol in human: hair cortisol as an endogenous biomarker
Abstract
BACKGROUND: Cortisol level in human hair would be an endogenous biomarker for the retrospective assessment of long-term central hypothalamo-pituitary-adrenal activity. However, no direct evidence supports that blood-related diffusion is a biologically endogenous source of hair cortisol in humans. The present study aims to validate the direct correlation between cortisol in 1-cm hair segments and salivary cortisol in healthy humans. METHODS: We collected three saliva samples from the same participant at Time 1, Time 2 (1 week later) and Time 3 (2 weeks later), and hair 4 weeks later. Cortisol levels in 1-cm hair segments and saliva were determined with high performance liquid chromatography tandem mass spectrometry. RESULTS: Salivary cortisol at Time 1 was significantly associated with that at Time 2 (r=0.514, p=0.003), but not with that at Time 3 (r=0.187, p=0.305); and the one at Time 2 was significantly associated with that at Time 3 (r=0.380, p=0.032). Hair cortisol was significantly correlated with salivary cortisol at Time 2 (r=0.389, p<0.05) and average salivary cortisol (r=0.383, p<0.05) from three sampling. CONCLUSIONS: Our results confirmed that blood-related diffusion mechanism is a biologically endogenous source of hair cortisol.
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Three independent published samples report cortisol decline rates per 1-cm hair segment of 2.5 pg/mg, 2.7±0.3 pg/mg, and 2.9±0.6 pg/mg.
“Three independent, published samples report cortisol decline rates per 1-cm hair segment of 2.5 pg/mg (Kirschbaum et al., 2009), 2.7±0.3 pg/mg (Gao et al., 2010), and 2.9±0.6 pg/mg (Xie et al., 2011).”
From Chronic Stress, Hair Cortisol and Depression: A Prospective and Longitudinal Study of Medical Internship (2018) · cites this paper as Xie et al., 2011
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Self-citation
- Authors
- Xie, Q., Gao, W., Li, J., Qiao, T., Jin, J., Deng, H., & Lu, Z.
- Affiliations
- Southeast University (CN)
- Corresponding author
- Deng, Huihua
- Venue
- Clinical Chemistry and Laboratory Medicine (CCLM)
- Publisher
- Walter de Gruyter GmbH
- Published
- 2011-01-01
- Volume
- 49
- Issue
- 12
- Pages
- 2013-9
- ISSN
- 1434-6621 (ISSN-L)
- 1437-4331
- OpenAlex
- W2052995288
- Cited by
- 85
- References
- 16
- Citations by year
- 2025: 2 · 2024: 6 · 2023: 8 · 2022: 2 · 2021: 4 · 2020: 11 · 2019: 9 · 2018: 5 · 2017: 8 · 2016: 8 · 2015: 4 · 2014: 5 · 2013: 6 · 2012: 7
- FWCI
- 2.25
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Clinical Chemistry and Laboratory Medicine (CCLM) (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China
- Program for New Century Excellent Talents in University
- Topics
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Medicinal Plants and Bioactive Compounds
- Keywords
- hair cortisol, salivary cortisol, cortisol, hypothalamic-pituitary-adrenal axis, chronic stress, biomarker validation
- MeSH terms
- Adult, Chromatography, High Pressure Liquid, Female, Hair, Humans, Hydrocortisone, Male, Retrospective Studies, Saliva, Biomarkers, Tandem Mass Spectrometry, Young Adult
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