Catechol oxidation by peroxidase-positive astrocytes in primary culture: an electron spin resonance study
Open accessAbstract
In rodents, chronic estrogenization has been shown to induce degeneration of dendrites and myelin figures in the hypothalamic arcuate nucleus adjacent to peroxidase-positive astrocyte processes. Because in this brain region estradiol is metabolized to 2-hydroxyestradiol (catecholestrogen), we hypothesized that the latter may be oxidized by the astrocytic peroxidase activity to cytotoxic ortho-semiquinones as occurs in peripheral tissues. Cysteamine induces nonenzymatic peroxidase activity in cultured astroglia identical to that observed in vivo. Using electron spin resonance, we demonstrate robust peroxidase-catalyzed oxidation of 2-hydroxyestradiol and dopamine by cysteamine-pretreated astrocyte cultures relative to untreated controls. These results implicate the peroxidase-positive astrocytes in the pathogenesis of estradiol-related hypothalamic damage, parkinsonism, and other free-radical-related neurologic disorders.
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Catecholamines, such as dopamine, might be oxidised to highly reactive or toxic quinones via the reduction of ferric iron or enzymatically.
“Cathecolamines, such as dopamine, might be oxidised to highly reactive or toxic quinones via the reduction of ferric iron or enzymatically.65–67”
From The role of iron in brain ageing and neurodegenerative disorders (2014) · cites this paper as [65]
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Cites
Oxidative Pathways for Catecholamines in the Genesis of Neuromelanin and Cytotoxic Quinones
GRAHAM, D. G. (1978) · Molecular Pharmacology · doi:10.1016/s0026-895x(25)13535-9 · Cited by 1,126 · cited 1× · see citing works
“…cultures (Schipper et al., 1990b), some degree of autoxidation that occurs at neutral pH, and the enzymatic activities of various oxidases and peroxidases (Graham , 1978; Graham et al., 1978; Rosenberg, 1988) may all contribute to the low level of catechol oxidation in the control cell incubations.”
BackgroundKontos, H. A., Wei, E. P., Ellis, E. F., Jenkins, L. W., Povlishock, J. T., Rowe, G. T., & Hess, M. L. (1985) · Circulation Research · doi:10.1161/01.res.57.1.142 · Cited by 291 · see citing works
Brawer, J. R., Naftolin, F. N., Martin, J. B., & Sonnenschein, C. (1978) · Endocrinology · doi:10.1210/endo-103-2-501 · Cited by 225 · see citing works
Synaptic remodeling in the rat arcuate nucleus during the estrous cycle
Olmos, G., Naftolin, F. N., Perez, J., Tranque, P., & García‐Segura, L. M. (1989) · Neuroscience · doi:10.1016/0306-4522(89)90288-1 · Cited by 202 · see citing works
Ovary-Dependent Degeneration in the Hypothalamic Arcuate Nucleus*
Brawer, J. R., Schipper, H. M., & Naftolin, F. N. (1980) · Endocrinology · doi:10.1210/endo-107-1-274 · Cited by 133 · see citing works
Self-citationEffects of Long Term Androgen and Estradiol Exposure on the Hypothalamus*
Brawer, J. R., Schipper, H. M., & Robaire, B. (1983) · Endocrinology · doi:10.1210/endo-112-1-194 · Cited by 131 · see citing works
Self-citationEtiology of Parkinson’s Disease: A Research Strategy
Barbeau, A. (1984) · Canadian Journal of Neurological Sciences / Journal Canadien des Sciences Neurologiques · doi:10.1017/s0317167100045273 · Cited by 109 · see citing works
Role of the Gonads in the Histologic Aging of the Hypothalamic Arcuate Nucleus1
Schipper, H. M., Brawer, J. R., Nelson, F., Felicio, L. S., & Finch, C. E. (1981) · Biology of Reproduction · doi:10.1095/biolreprod25.2.413 · Cited by 97 · see citing works
Self-citationThe cellular effects of estrogens on neuroendocrine tissues
Naftolin, F., Maclusky, N. J., Leranth, C. Z., Sakamoto, H. S., & Garcia-Segura, L. M. (1988) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(88)90093-3 · Cited by 86 · see citing works
Distribution of endogenous diaminobenzidine-staining cells in the normal rat brain
Keefer, D. A., & Christ, J. F. (1976) · Brain Research · doi:10.1016/0006-8993(76)90909-4 · Cited by 59 · see citing works
Schipper, H. M., Lechan, R. M., & Reichlin, S. (1990) · Brain Research · doi:10.1016/0006-8993(90)90273-e · Cited by 50 · see citing works
Self-citationEvidence for a peroxidatic oxidation of norepinephrine, a catecholamine, by lactoperoxidase
Metodiewa, D., Reszka, K. J., & Dunford, H. B. (1989) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(89)80128-7 · Cited by 42 · see citing works
Gomori-positive astrocytes in primary culture: effects of in vitro age and cysteamine exposure
Schipper, H. M., Scarborough, D. E., Lechan, R. M., & Reichlin, S. (1990) · Developmental Brain Research · doi:10.1016/0165-3806(90)90066-8 · Cited by 33 · see citing works
Self-citationPeriventricular gomori-positive glia in brains of X-irradiated rats
Srebro, Z. (1971) · Brain Research · doi:10.1016/0006-8993(71)90488-4 · Cited by 29 · see citing works
Goldgefter, L., Schejter, A., & Gill, D. (1980) · Cell and Tissue Research · doi:10.1007/bf00234404 · Cited by 24 · see citing works
Abul‐Hajj, Y. J., & Cisek, P. L. (1988) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(88)90212-9 · Cited by 18 · see citing works
Studies on the structure and function of Gomori-positive glial cells in the rat hypothalamus
Goldgefter, L. (1976) · Cells Tissues Organs · doi:10.1159/000144642 · Cited by 16 · see citing works
Histochemical study on endogenous diaminobenzidine-positive granules in the glia cell of rat brain.
Kumamoto, T. (1981) · ACTA HISTOCHEMICA ET CYTOCHEMICA · doi:10.1267/ahc.14.173 · Cited by 9 · see citing works
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- Cohen G (1987) Oxygen radicals and Parkinson's disease. Upjohn Symposium on Oxygen Radicals (Halliwell B, ed.), pp 130-l 35. Au- gusta, MI.
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- Authors
- Schipper, H., Kotake, Y., & Janzen, E.
- Affiliations
- Jewish General Hospital (CA)
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 1991-07-01
- Volume
- 11
- Issue
- 7
- Pages
- 2170-2176
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6575459
- OpenAlex
- W1756695389
- Cited by
- 58
- References
- 19
- Citations by year
- 2023: 2 · 2022: 1 · 2020: 1 · 2019: 2 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 1 · 2014: 1
- FWCI
- 1.59
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Parkinson's Disease Foundation
- Medical Research Council Canada
- Parkinsonfonden
- Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Neurology › Parkinson's Disease Mechanisms and Treatments
- Keywords
- catechol oxidation, 2-hydroxyestradiol, catechol estrogens, cysteamine, free radicals, hypothalamic arcuate nucleus, dopamine oxidation, astrocytes, parkinsonism
- MeSH terms
- Animals, Astrocytes, Brain, Cells, Cultured, Cysteamine, Dopamine, Electron Spin Resonance Spectroscopy, Estradiol, Fluorescent Antibody Technique, Glial Fibrillary Acidic Protein, Histocytochemistry, Peroxidase, NADP, Oxidation-Reduction, Rats, Inbred Strains, Staining and Labeling, 3,3'-Diaminobenzidine, Benzoquinones, Rats
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