The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Multiple Structural Elements Determine Subunit Specificity of Mg2+Block in NMDA Receptor Channels
Open accessAbstract
In NMDA receptor channels, subtype-specific differences of Mg 2+block are determined by the NR2 subunits. Channels assembled from the NR1-NR2A or NR1-NR2B subunits are blocked more strongly than channels formed by the NR1-NR2C or NR1-NR2D subunits, predominantly reflecting a difference in voltage dependence. A determinant of Mg 2+block common to the NR2 subunits is located in the M2 domain (N-site or Q/R/N-site). However, subunit-specific differences of block suggested that additional structural elements exist. Chimeric NR2 subunits were constructed by replacing segments of the least sensitive NR2C subunit with homologous segments of the most sensitive NR2B subunit. Mutant NR2 subunits were coexpressed with wild-type NR1 in Xenopus oocytes, and Mg 2+block was quantified. Replacement of the entire M1–M4 region resulted in a chimera with a sensitivity of Mg 2+block similar to that of the NR2B wild type. Replacing smaller segments or introducing point mutations did not generate channels with Mg 2+block characteristic of NR2B wild type. However, combining in a single chimera three small segments (M1, M2–M3 linker, M4), each independently mediating an increase in Mg 2+block, produced channels close to NR2B wild type. Thus, differences in Mg 2+block as controlled by the NR2 subunits cannot be explained by a single structural determinant in addition to the N-site. Moreover, three elements of the NR2 subunit are the major determinants of subtype-specific differences of Mg 2+block in heteromeric NMDA receptor channels.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The cited source says what the claim attributes to it.
In the citing paper
“Notably, the magnitude of Mg2+ block and Ca2+ permeability is dependent on the subunit composition of the receptor”
From Mechanisms of ketamine and its metabolites as antidepressants (2022) · cites this paper as Kuner & Schoepfer, 1996
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A synaptic model of memory: long-term potentiation in the hippocampus
Bliss, T. V. P., & Collingridge, G. L. (1993) · Nature · doi:10.1038/361031a0 · Cited by 11,566
Glutamate neurotoxicity and diseases of the nervous system
Choi, D. W. (1988) · Neuron · doi:10.1016/0896-6273(88)90162-6 · Cited by 8,188
Magnesium gates glutamate-activated channels in mouse central neurones
Nowak, L. M., Bregestovski, P. D., Ascher, P., Herbet, A., & Prochiantz, A. (1984) · Nature · doi:10.1038/307462a0 · Cited by 4,116
Hollmann, M., & Heinemann, S. F. (1994) · Annual Review of Neuroscience · doi:10.1146/annurev.ne.17.030194.000335 · Cited by 4,056
Monyer, H., Burnashev, N., Laurie, D. J., Sakmann, B., & Seeburg, P. H. (1994) · Neuron · doi:10.1016/0896-6273(94)90210-0 · Cited by 3,535
Voltage-dependent block by Mg2+ of NMDA responses in spinal cord neurones
Mayer, M. L., Westbrook, G. L., & Guthrie, P. B. (1984) · Nature · doi:10.1038/309261a0 · Cited by 2,941
Heteromeric NMDA receptors: molecular and functional distinction of subtypes
Monyer, H., Sprengel, R., Schoepfer, R., Herb, A., Higuchi, M., Lomeli, H., Burnashev, N., Sakmann, B., & Seeburg, P. H. (1992) · Science · doi:10.1126/science.256.5060.1217 · Cited by 2,541
Self-citationMolecular cloning and characterization of the rat NMDA receptor
Moriyoshi, K., Masu, M., Ishii, T., Shigemoto, R., Mizuno, N., & Nakanishi, S. (1991) · Nature · doi:10.1038/354031a0 · Cited by 1,771
Ionic Blockage of Sodium Channels in Nerve
Woodhull, A. M. (1973) · The Journal of General Physiology · doi:10.1085/jgp.61.6.687 · Cited by 1,581
Molecular diversity of the NMDA receptor channel
Kutsuwada, T., Kashiwabuchi, N., Mori, H., Sakimura, K., Kushiya, E., Araki, K., Meguro, H., Hisashi, M., Kumanishi, T., Arakawa, M., et al. (1992) · Nature · doi:10.1038/358036a0 · Cited by 1,352
Modulation of Neuronal Migration by NMDA Receptors
Komuro, H., & RAKIC, P. T. (1993) · Science · doi:10.1126/science.8096653 · Cited by 1,041
Molecular characterization of the family of the N-methyl-D-aspartate receptor subunits.
Ishii, T. M., Moriyoshi, K., Sugihara, H., Sakurada, K., Kadotani, H., Yokoi, M., Akazawa, C., Shigemoto, R., Mizuno, N., & Masu, M. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)53849-7 · Cited by 980
N-methyl-D-aspartic acid receptor structure and function
McBain, C. J., & Mayer, M. L. (1994) · Physiological Reviews · doi:10.1152/physrev.1994.74.3.723 · Cited by 976
Protein kinase C reduces Mg2+ block of NMDA-receptor channels as a mechanism of modulation
Chen, L., & Huang, L.-Y. M. (1992) · Nature · doi:10.1038/356521a0 · Cited by 974
Mayer, M. L., & Westbrook, G. L. (1987) · The Journal of Physiology · doi:10.1113/jphysiol.1987.sp016883 · Cited by 943
Excitatory amino acid receptors and synaptic plasticity
Collingridge, G. L., & Singer, W. (1990) · Trends in Pharmacological Sciences · doi:10.1016/0165-6147(90)90011-v · Cited by 867
Ascher, P., & Nowak, L. (1988) · The Journal of Physiology · doi:10.1113/jphysiol.1988.sp017078 · Cited by 819
Functional characterization of a heteromeric NMDA receptor channel expressed from cloned cDNAs
Meguro, H., Mori, H., Araki, K., Kushiya, E., Kutsuwada, T., Yamazaki, M., Kumanishi, T., Arakawa, M., Sakimura, K., & Mishina, M. (1992) · Nature · doi:10.1038/357070a0 · Cited by 799
Impulse activity and the patterning of connections during cns development
Shatz, C. J. (1990) · Neuron · doi:10.1016/0896-6273(90)90333-b · Cited by 766
Developmental changes in distribution of NMDA receptor channel subunit mRNAs
Watanabe, M., Inoue, Y., Sakimura, K., & Mishina, M. (1992) · Neuroreport · doi:10.1097/00001756-199212000-00027 · Cited by 721
Zinc potentiates agonist-lnduced currents at certain splice variants of the NMDA receptor
Hollmann, M., Boulter, J., Maron, C., Beasley, L., Sullivan, J., Pecht, G., & Heinemann, S. F. (1993) · Neuron · doi:10.1016/0896-6273(93)90209-a · Cited by 614
Akazawa, C., Shigemoto, R., Bessho, Y., Nakanishi, S., & Mizuno, N. (1994) · The Journal of Comparative Neurology · doi:10.1002/cne.903470112 · Cited by 506
Burnashev, N., Zhou, Z., Neher, E., & Sakmann, B. J. (1995) · The Journal of Physiology · doi:10.1113/jphysiol.1995.sp020738 · Cited by 465
Köhler, M. G., Burnashev, N., Sakmann, B. J., & Seeburg, P. H. (1993) · Neuron · doi:10.1016/0896-6273(93)90336-p · Cited by 464
Control by Asparagine Residues of Calcium Permeability and Magnesium Blockade in the NMDA Receptor
Burnashev, N., Schoepfer, R., Monyer, H., Ruppersberg, J. P., Gunther, W. C., Seeburg, P. H., & Sakmann, B. J. (1992) · Science · doi:10.1126/science.1382314 · Cited by 455
Self-citationStern-Bach, Y., Bettler, B., Hartley, M., Sheppard, P. O., O’Hara, P. J., & Heinemann, S. F. (1994) · Neuron · doi:10.1016/0896-6273(94)90420-0 · Cited by 432
Hollmann, M., Maron, C., & Heinemann, S. (1994) · Neuron · doi:10.1016/0896-6273(94)90419-7 · Cited by 421
A quantitative description of NMDA receptor-channel kinetic behavior
Jahr, C. E., & Stevens, C. F. (1990) · Journal of Neuroscience · doi:10.1523/jneurosci.10-06-01830.1990 · Cited by 327
Cloning and expression of the ε4 subunit of the NMDA receptor channel
Ikeda, K., Nagasawa, M., Mori, H., Araki, K., Sakimura, K., Watanabe, M., Inoue, Y., & Mishina, M. (1992) · FEBS Letters · doi:10.1016/0014-5793(92)81178-o · Cited by 299
Cloning, expression and modulation of a mouse NMDA receptor subunit
Yamazaki, M., Mori, H., Araki, K., Mori, K. J., & Mishina, M. (1992) · FEBS Letters · doi:10.1016/0014-5793(92)80160-i · Cited by 290
Bennett, J. A., & Dingledine, R. J. (1995) · Neuron · doi:10.1016/0896-6273(95)90293-7 · Cited by 288
Stern, P., Béhé, P., Schoepfer, R., & Colquhoun, D. (1992) · Proceedings of the Royal Society B Biological Sciences · doi:10.1098/rspb.1992.0159 · Cited by 267
Self-citationIdentification by mutagenesis of a Mg2+ -block site of the NMDA receptor channel
Mori, H., Hisashi, M., Yamakura, T., & Mishina, M. (1992) · Nature · doi:10.1038/358673a0 · Cited by 262
Transmembrane topology of two kainate receptor subunits revealed by N-glycosylation.
Wo, Z. G., & Oswald, R. E. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.15.7154 · Cited by 195
DiFrancesco, D. (1982) · The Journal of Physiology · doi:10.1113/jphysiol.1982.sp014315 · Cited by 183
Kato, N., Artola, A., & Singer, W. (1991) · Developmental Brain Research · doi:10.1016/0165-3806(91)90153-a · Cited by 156
Sakurada, K., Masu, M., & Nakanishi, S. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)54166-1 · Cited by 155
Regulation of hippocampal NMDA receptors by magnesium and glycine during development.
Kleckner, N. W., & Dingledine, R. J. (1991) · PubMed · Cited by 125
Leonard, J. P., & Kelso, S. R. (1990) · Neuron · doi:10.1016/0896-6273(90)90443-j · Cited by 124
The NMDA Receptor Channel: Molecular Design of a Coincidence Detector
SEEBURG, P. H., BURNASHEV, N., KÖHR, G., KUNER, T., SPRENGEL, R., & MONYER, H. (1995) · Proceedings of the 1993 Laurentian Hormone Conference · doi:10.1016/b978-0-12-571150-0.50006-8 · Cited by 118
Self-citationEthanol inhibits glutamate-induced currents in heteromeric NMDA receptor subtypes
Kuner, T., Schoepfer, R., & Korpi, E. R. (1993) · Neuroreport · doi:10.1097/00001756-199312000-00029 · Cited by 95
Self-citationMultiple structural determinants of voltage-dependent magnesium block in recombinant NMDA receptors
Shinichi, K., & Dingledine, R. J. (1993) · Neuropharmacology · doi:10.1016/0028-3908(93)90014-t · Cited by 95
Kato, N., & Yoshimura, H. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.15.7114 · Cited by 79
Structural basis of ion channel permeation and selectivity
Sather, W. A., Yand, J., & Tsien, R. W. (1994) · Current Opinion in Neurobiology · doi:10.1016/0959-4388(94)90091-4 · Cited by 77
NMDA Receptor as a Mediator of Activity-dependent Synaptogenesis in the Developing BrainPreprint
Constantine‐Paton, M. (1990) · Cold Spring Harbor Symposia on Quantitative Biology · doi:10.1101/sqb.1990.055.01.043 · Cited by 74
The mechanism of magnesium block of NMDA receptors
Ruppersberg, J. P., von Kitzing, E., & Schoepfer, R. (1994) · Seminars in Neuroscience · doi:10.1006/smns.1994.1012 · Cited by 58
Self-citationFerrer-Montiel, A. V., Sun, W. Y., & Montal, M. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.17.8021 · Cited by 46
Reduced Mg^ block of NMDA receptor-mediated synaptic potentials in developing visual cortex
Kato, N. (1993) · Medical Entomology and Zoology · Cited by 38
Nabekura, J., Kawamoto, I., & Akaike, N. (1994) · Brain Research · doi:10.1016/0006-8993(94)91915-1 · Cited by 22
Research works printed but not linked: 3
- Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1995) Mutagenesis of cloned DNA. In: Current protocols in molecular biology, Chap 8. (Janssen K, ed). New York: Wiley.
- Kuner, 1995, Probing the cytoplasmic face of the NMDA receptor channel pore in cysteine-substitution mutants., Soc Neurosci Abstr, 21, 85
- Schoepfer, 1994, Single channel properties of recombinant NMDA receptors expressed in Xenopus oocytes., Soc Neurosci Abstr, 20, 309.2
Cited by
Sorted by citations
Glutamate receptor ion channels
Mayer, M. L. (2005) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2005.05.004 · Cited by 3,655
Glutamate Receptor Ion Channels: Structure, Regulation, and Function
Traynelis, S. F., Wollmuth, L. P., McBain, C. J., Menniti, F. S., Vance, K. M., Ogden, K. K., Hansen, K. B., Yuan, H., Myers, S. J., & Dingledine, R. (2010) · Pharmacological Reviews · doi:10.1124/pr.109.002451 · Cited by 3,568
The Glutamate Receptor Ion Channels
Dingledine, R., Borges, K., Bowie, D., & Traynelis, S. F. (1999) · Pharmacological Reviews · doi:10.1016/s0031-6997(24)01394-2 · Cited by 3,554
The Emerging Role of Glutamate in the Pathophysiology and Treatment of Schizophrenia
GOFF, D. C., & Coyle, J. T. (2001) · American Journal of Psychiatry · doi:10.1176/appi.ajp.158.9.1367 · Cited by 926
NMDA receptor subunits: function and pharmacology
PAOLETTI, P., & NEYTON, J. (2007) · Current Opinion in Pharmacology · doi:10.1016/j.coph.2006.08.011 · Cited by 887
Metadata
- Authors
- Kuner, T., & Schoepfer, R.
- Affiliations
- Heidelberg University (DE)
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 1996-06-01
- Volume
- 16
- Issue
- 11
- Pages
- 3549-3558
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6578835
- OpenAlex
- W1530479094
- Cited by
- 235
- References
- 49
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 7 · 2023: 5 · 2022: 5 · 2021: 8 · 2020: 9 · 2019: 5 · 2018: 6 · 2017: 4 · 2016: 10 · 2015: 3 · 2014: 8 · 2013: 9 · 2012: 10
- FWCI
- 4.60
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Max Planck Digital Library (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Funders
- Wellcome Trust
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Keywords
- NMDA receptor channels, Mg2+ block, NR2 subunits, subunit specificity, NR2B, NR2C, NR1, chimeric subunits, voltage dependence, heteromeric channels, ion channel structure
- MeSH terms
- Amino Acid Sequence, Animals, Female, Ion Channels, Magnesium, Microelectrodes, Molecular Sequence Data, Oocytes, Recombinant Fusion Proteins, Sensitivity and Specificity, Xenopus, Receptors, N-Methyl-D-Aspartate, Patch-Clamp Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
