The DOI matches its Crossref record; 77 of the 79 references it cites were found in a registry or an index.
Postsynaptic Scaffolds of Excitatory and Inhibitory Synapses in Hippocampal Neurons: Maintenance of Core Components Independent of Actin Filaments and Microtubules
Open accessAbstract
The mechanisms responsible for anchoring molecular components of postsynaptic specializations in the mammalian brain are not well understood but are presumed to involve associations with cytoskeletal elements. Here we build on previous studies of neurotransmitter receptors (Allison et al., 1998) to analyze the modes of attachment of scaffolding and signal transducing proteins of both glutamate and GABA postsynaptic sites to either the microtubule or microfilament cytoskeleton. Hippocampal pyramidal neurons in culture were treated with latrunculin A to depolymerize actin, with vincristine to depolymerize microtubules, or with Triton X-100 to extract soluble proteins. The synaptic clustering of PSD-95, a putative NMDA receptor anchoring protein and a core component of the postsynaptic density (PSD), was unaffected by actin depolymerization, microtubule depolymerization, or detergent extraction. The same was largely true for GKAP, a PSD-95-interacting protein. In contrast, the synaptic clustering of Ca(2+)/calmodulin-dependent protein kinase II (CaMKII)alpha, another core component of the PSD, was completely dependent on an intact actin cytoskeleton and was partially disrupted by detergent. Drebrin and alpha-actinin-2, actin-binding proteins concentrated in spines, were also dependent on F-actin for synaptic localization but were unaffected by detergent extraction. Surprisingly, the subcellular distributions of the inhibitory synaptic proteins GABA(A)R and gephyrin, which has a tubulin-binding motif, were unaffected by depolymerization of microtubules or actin or by detergent extraction. These studies reveal an unsuspected heterogeneity in the modes of attachment of postsynaptic proteins to the cytoskeleton and support the idea that PSD-95 and gephyrin may be core scaffolding components independent of the actin or tubulin cytoskeleton.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Lastly, the actin cytoskeleton is known to mediate receptor positioning (Gudheti et al., 2013) and its depolymerization was shown to reduce glutamate receptor accumulations in spines (Allison et al., 2000).”
From Barriers in the brain: resolving dendritic spine morphology and compartmentalization (2014) · cites this paper as Allison et al., 2000
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Domain Interaction Between NMDA Receptor Subunits and the Postsynaptic Density Protein PSD-95
Kornau, H., Schenker, L. T., Kennedy, M. B., & Seeburg, P. H. (1995) · Science · doi:10.1126/science.7569905 · Cited by 1,916
(1991) · Medical Entomology and Zoology · Cited by 1,512
Dendritic spine changes associated with hippocampal long-term synaptic plasticity
Engert, F., & Bonhoeffer, T. (1999) · Nature · doi:10.1038/19978 · Cited by 1,412
Rat hippocampal neurons in dispersed cell culture.
Banker, G. A., & Cowan, W. M. (1977) · Brain Research · doi:10.1016/0006-8993(77)90594-7 · Cited by 1,110
Harris, K. M., & Kater, S. B. (1994) · Annual Review of Neuroscience · doi:10.1146/annurev.ne.17.030194.002013 · Cited by 1,020
Naisbitt, S., Kim, E., Tu, J. C., Xiao, B., Sala, C., Valtschanoff, J., Weinberg, R. J., Worley, P. F., & Sheng, M. (1999) · Neuron · doi:10.1016/s0896-6273(00)80809-0 · Cited by 1,009
Postsynaptic clustering of major GABAA receptor subtypes requires the γ2 subunit and gephyrin
Essrich, C., Lorez, M., Benson, J. A., Fritschy, J., & Lüscher, B. (1998) · Nature Neuroscience · doi:10.1038/2798 · Cited by 856
GABAA-receptor-associated protein links GABAA receptors and the cytoskeleton
Wang, H.-B., Bedford, F., Brandon, N., Moss, S., & Olsen, R. (1999) · Nature · doi:10.1038/16264 · Cited by 754
Shen, K., & Meyer, T. (1999) · Science · doi:10.1126/science.284.5411.162 · Cited by 673
Competitive binding of α-actinin and calmodulin to the NMDA receptor
Wyszynski, M., Lin, J., Rao, A., Nigh, E., Beggs, A. H., Craig, A. M., & Sheng, M. (1997) · Nature · doi:10.1038/385439a0 · Cited by 596
Self-citation
Cited by
Sorted by citations
Items 201–243 of 243
Scaffold Proteins in the Postsynaptic Density
Kennedy, M. B., Marcora, E. M., & Carlisle, H. J. (2008) · Springer eBooks · doi:10.1007/978-0-387-77232-5_14 · Cited by 2
Rodriguez, P. C., Almeida, L. G., & Triller, A. (2017) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/193698 · Cited by 2
Synaptic proteins expressed by oligodendrocytes mediate CNS myelinationPreprint
Hughes, A., & Appel, B. (2018) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/410969 · Cited by 2
Chen, X., Pan, D., Liu, J., & Yang, Y. (2024) · eLife · doi:10.7554/elife.102792 · Cited by 2
Accelerators, Brakes, and Gears of Actin Dynamics in Dendritic Spines
Pontrello, C. G., & Ethell, I. M. (2014) · The Open Neuroscience Journal · doi:10.2174/1874082000903010067 · Cited by 1
Dendritic Organelles for Postsynaptic Trafficking
Hanus, C., & Ehlers, M. (2008) · Springer eBooks · doi:10.1007/978-0-387-77232-5_8 · Cited by 1
MOLECULAR GENETICS OF ACTIVITY-DEPENDENT STRUCTURAL CHANGES AT THE SYNAPSE
Devaud, J., & Ferrús, A. (2003) · Journal of Neurogenetics · doi:10.1080/01677060390441840 · Cited by 2
Regulation of Oligodendrocyte differentiation through Neuroligin-Neurexin signalling
Scott, L. O. M. (2013) · Libraries and Cultural Resources (University of Calgary) · doi:10.11575/prism/27587 · Cited by 2
Plasticity at the synapes: a role for microRNAs
Ori, A. (2011) · Utrecht University Repository (Utrecht University)
Neurexins and neuroligins: new partners for GABAA receptors at synapses
Wu, B., & Zhang, C. (2011) · Frontiers in Biology · doi:10.1007/s11515-011-1020-2
Identification of novel target genes for the plasticity-related transcription factor Zif268
McDade, D. M. (2007) · Enlighten: Theses (The University of Glasgow)
Lareo, L. (2006) · LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas)
Actin cytoskeleton in dendritic spine
Hanamura, K., & Shirao, T. (2007) · Folia Pharmacologica Japonica · doi:10.1254/fpj.130.352
The Actin Filament System : Its Involvement in Cell Migration and Neurotransmitter Release
Johnsson, A. (2011) · DiVA (Stockholm University)
Jessen, A. L. (2010) · eDiss (Georg-August-Universität Göttingen) · doi:10.53846/goediss-406
Role of Neuroligins at the Inhibitory Postsynaptic Compartment of the Retina
Hoon, M. (2010) · eDiss (Georg-August-Universität Göttingen) · doi:10.53846/goediss-3305
Trafficking of Postsynaptic GABAA Receptors by Receptor-Associated Proteins
Yuan, X., & Lüscher, B. (2007) · Humana Press eBooks · doi:10.1007/978-1-59745-465-0_3
Mechanisms of Synapse Formation and Synaptic Plasticity
Luk, C. (2015) · Libraries and Cultural Resources (University of Calgary) · doi:10.11575/prism/26867
Regulatory Crosstalk Analysis of Biochemical Networks in the Hippocampus and Nucleus Accumbens
Kikuchi, S., & Voit, E. O. (2013) · Springer eBooks · doi:10.1007/978-94-007-6803-1_4
van Zundert, B., Álvarez, F. J., Tapia, J. C., Yeh, H. H., Emílio, & Aguayo, L. G. (2003)
Roles of CaMKII in Learning and Memory ☆
Stephenson, J. R., & Colbran, R. J. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-809324-5.21106-1
Effect of Vincristine and Vinblastine from Vinca Rosa on Microtubulues of Tumor H22 Cell Line
Mohammed, I. H. (2012) · DOAJ (DOAJ: Directory of Open Access Journals)
Construction, Stability and Dynamics of the Inhibitory Postsynaptic Membrane
Vannier, C., & Triller, A. (2003) · Springer eBooks · doi:10.1007/978-1-4615-0039-1_4
Ziv, N. E. (2007) · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature · doi:10.3410/f.1086979.539935
Molecular Mechanisms Involved In The Generation Of Inhibitory Postsynaptic Structures
Wagner, S. (2021) · eDiss (Georg-August-Universität Göttingen) · doi:10.53846/goediss-8714
Actin and Actin-Binding Proteins: Masters of Dendritic Spine Formation, Morphology, and Function
Lin, W., & Webb, D. J. (2014) · The Open Neuroscience Journal · doi:10.2174/1874082000903010054
Chen, X., Pan, D., Liu, J., & Yang, Y. (2025) · eLife · doi:10.7554/elife.102792.4
Chen, X., Pan, D., Liu, J., & Yang, Y. (2024) · eLife · doi:10.7554/elife.102792.1
Chen, X., Pan, D., Liu, J., & Yang, Y. (2025) · eLife · doi:10.7554/elife.102792.2
Nexilin regulates cell surface expression of extrasynaptic GABAA receptors by binding to actin
Bright, D. P., Schulte, C., Halff, E. F., Lumb, M. J., Kittler, J. T., Maric, H. M., & Smart, T. G. (2025) · Neuropharmacology · doi:10.1016/j.neuropharm.2025.110633
Chen, X., Pan, D., Liu, J., & Yang, Y. (2025) · eLife · doi:10.7554/elife.102792.3
Castro, F. G. (2017) · theses.fr (ABES) · doi:10.70675/b2431e98z12ecz49baz8692z44cb7b3fb5ed
Rôle des variants d’épissage de la géphyrine dans la diversité synaptique
Reis, R. D. (2021) · theses.fr (ABES) · doi:10.70675/68262ae7z2347z4296zbaa1zb4fe4ec67901
The Importance of Glia-Synapse Interactions in Synaptic Connectivity
Haider, T. (2014) · Utrecht University Repository (Utrecht University)
Beyond G proteins: The role of accessory proteins in G protein-coupled receptor signalling
Just, H., Stefan, E., Czupalla, C., Nürnberg, B., Nanoff, C., & Freissmuth, M. (2002) · Pharmacochemistry library · doi:10.1016/s0165-7208(02)80017-4
Metadata
- Authors
- Allison, D. W., Chervin, A. S., Gelfand, V. I., & Craig, A. M.
- Affiliations
- University of Illinois Urbana-Champaign (US)
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2000-06-15
- Volume
- 20
- Issue
- 12
- Pages
- 4545-4554
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6772458
- OpenAlex
- W2148256023
- Cited by
- 245
- References
- 79
- Citations by year
- 2025: 5 · 2024: 3 · 2023: 2 · 2022: 4 · 2021: 10 · 2020: 4 · 2019: 3 · 2018: 4 · 2017: 5 · 2016: 2 · 2015: 5 · 2014: 12 · 2013: 8 · 2012: 7
- FWCI
- 8.28
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- National Institutes of Health (GM-52111)
- National Institute of General Medical Sciences (R01GM052111, GM52111-01)
- National Institute of Neurological Disorders and Stroke (NS33184, R01NS033184)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- postsynaptic scaffolds, postsynaptic density, actin cytoskeleton, microtubules, PSD-95, GKAP, CaMKIIα, drebrin, ACTN2, GABAA receptor, gephyrin, glutamatergic synapses, inhibitory synapses, hippocampal pyramidal neurons, latrunculin A, vincristine, detergent extraction, dendritic spines
- MeSH terms
- Disks Large Homolog 4 Protein, Actins, Animals, Cells, Cultured, Evoked Potentials, Hippocampus, Marine Toxins, Membrane Proteins, Microtubules, Nerve Tissue Proteins, Neurons, Synapses, Thiazoles, Vincristine, Octoxynol, Bridged Bicyclo Compounds, Heterocyclic, Excitatory Postsynaptic Potentials, Intracellular Signaling Peptides and Proteins, Rats, Thiazolidines
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
