Remodeling of Synaptic Membranes after Induction of Long-Term Potentiation
Open accessAbstract
Several morphological changes of synapses have been reported to be associated with the induction of long-term potentiation (LTP) in the CA1 hippocampus, including an transient increase in the proportion of synapses with perforated postsynaptic densities (PSDs) and a later occurrence of multiple spine boutons (MSBs) in which the two spines arise from the same dendrite. To investigate the functional significance of these modifications, we analyzed single sections and reconstructed 134 synapses labeled via activity using a calcium precipitation approach. Analyses of labeled spine profiles showed changes of the spine head area, PSD length, and proportion of spine profiles containing a coated vesicle that reflected variations in the relative proportion of different types of synapses. Three-dimensional reconstruction indicated that the increase of perforated spine profiles observed 30 min after LTP induction essentially resulted from synapses exhibiting segmented, completely partitioned PSDs. These synapses had spine head and PSD areas approximately three times larger than those of simple synapses. They contained coated vesicles in a much higher proportion than that of any other type of synapse and exhibited large spinules associated with the PSD. Also the MSBs with two spines arising from the same dendrite that were observed 1-2 hr after LTP induction included a spine that was smaller and a PSD that was smaller than those of simple synapses. These results support the idea that LTP induction is associated with an enhanced recycling of synaptic membrane and that this process could underlie the formation of synapses with segmented PSDs and eventually result in the formation of a new, immature spine.
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At the ultrastructural level in electron micrographs, synapses that underwent LTP had enlarged PSDs that were often discontinuous.
“Furthermore, at the ultrastructural level in electron micrographs, synapses that underwent LTP had enlarged PSDs that were often discontinuous (Toni et al. 2001).”
From NMDA receptor-dependent long-term potentiation and long-term depression (LTP/LTD). (2012) · cites this paper as Toni et al. 2001
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“Branched spines have multiple heads originating from the same base spine and might reflect the beginning of a synapse duplication, leading to enhanced receptor turnover, which also can be observed during LTP (Hering and Sheng, 2001; Toni et al., 2001).”
InfluentialThree-dimensional synaptic Organization of Layer III of the human temporal neocortex
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- Authors
- Toni, N., Buchs, P.-A., Nikonenko, I., Povilaitite, P., Parisi, L., & Muller, D.
- Affiliations
- University of Geneva (CH)
- ORCID
- Toni, Nicolas
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2001-08-15
- Volume
- 21
- Issue
- 16
- Pages
- 6245-6251
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6763190
- OpenAlex
- W2106766347
- Cited by
- 244
- References
- 2
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 4 · 2023: 5 · 2022: 9 · 2021: 8 · 2020: 11 · 2019: 8 · 2018: 5 · 2017: 4 · 2016: 8 · 2015: 7 · 2014: 7 · 2013: 4 · 2012: 10
- FWCI
- 5.17
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Neuroscience (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Archive ouverte UNIGE (University of Geneva) (submittedVersion)
- Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
- Funders
- National Science Foundation
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (56852)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- long-term potentiation, hippocampal CA1, postsynaptic density, dendritic spines, spinules, coated vesicles, spine morphogenesis
- MeSH terms
- Animals, Calcium, Dendrites, Electric Stimulation, Hippocampus, Microscopy, Electron, Neuronal Plasticity, Synapses, Synaptic Membranes, Long-Term Potentiation, Coated Vesicles, Cell Surface Extensions, Rats, In Vitro Techniques
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