Cited by 292Epilepsy research and treatmentNeuroscience and Neuropharmacology ResearchProtease and Inhibitor Mechanisms

Important role of matrix metalloproteinase 9 in epileptogenesis

Open access
Wilczynski, Grzegorz M., Konopacki, Filip A., Wilczek, Ewa, Lasiecka, Zofia, Gorlewicz, Adam, Michaluk, Piotr, Wawrzyniak, Marcin, Malinowska, Monika +12 more·2008·The Journal of Cell Biology

Abstract

Temporal lobe epilepsy (TLE) is a devastating disease in which aberrant synaptic plasticity plays a major role. We identify matrix metalloproteinase (MMP) 9 as a novel synaptic enzyme and a key pathogenic factor in two animal models of TLE: kainate-evoked epilepsy and pentylenetetrazole (PTZ) kindling–induced epilepsy. Notably, we show that the sensitivity to PTZ epileptogenesis is decreased in MMP-9 knockout mice but is increased in a novel line of transgenic rats overexpressing MMP-9. Immunoelectron microscopy reveals that MMP-9 associates with hippocampal dendritic spines bearing asymmetrical (excitatory) synapses, where both the MMP-9 protein levels and enzymatic activity become strongly increased upon seizures. Further, we find that MMP-9 deficiency diminishes seizure-evoked pruning of dendritic spines and decreases aberrant synaptogenesis after mossy fiber sprouting. The latter observation provides a possible mechanistic basis for the effect of MMP-9 on epileptogenesis. Our work suggests that a synaptic pool of MMP-9 is critical for the sequence of events that underlie the development of seizures in animal models of TLE.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1083/jcb.200708213

Cites

57 of 57 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Wilczynski, G. M., Konopacki, F. A., Wilczek, E., Lasiecka, Z., Gorlewicz, A., Michaluk, P., Wawrzyniak, M., Malinowska, M., Okulski, P., Kolodziej, L. R., Konopka, W., Duniec, K., Mioduszewska, B., Nikolaev, E., Walczak, A., Owczarek, D., Gorecki, D. C., Zuschratter, W., Ottersen, O. P., & Kaczmarek, L.
Affiliations
  • Instytut Biologii Doświadczalnej im. Marcelego Nenckiego (PL)
  • Medical University of Warsaw (PL)
  • 1Department of Neurophysiology and
  • 6Institute of Biomedical and Biomolecular Sciences, University of Portsmouth, PO1 2DT Portsmouth, England, UK
  • Leibniz Institute for Neurobiology (DE)
  • University of Oslo (NO)
Venue
The Journal of Cell Biology
Published
2008-03-10
Volume
180
Issue
5
Pages
1021-1035
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
OpenAlex
W2102444756
Cited by
292
References
57
Citations by year
2026: 7 · 2025: 6 · 2024: 13 · 2023: 19 · 2022: 11 · 2021: 15 · 2020: 15 · 2019: 18 · 2018: 21 · 2017: 13 · 2016: 17 · 2015: 19 · 2014: 22 · 2013: 23 · 2012: 22
FWCI
11.95
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • The Journal of Cell Biology (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
Funders
Wellcome Trust
Topics
  • Health Sciences › Medicine › Psychiatry and Mental health › Epilepsy research and treatment
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
Keywords
MMP-9, epileptogenesis, temporal lobe epilepsy, synaptic plasticity, dendritic spines, mossy fiber sprouting, synaptogenesis, kainate-induced epilepsy, pentylenetetrazole kindling, MMP-9 knockout mice, hippocampus, seizures
MeSH terms
Animals, Convulsants, Disease Models, Animal, Epilepsy, Hippocampus, Male, Mice, Inbred C57BL, Neural Pathways, Neuronal Plasticity, Organ Culture Techniques, Synapses, Microscopy, Immunoelectron, Rats, Wistar, Mice, Knockout, Mossy Fibers, Hippocampal, Matrix Metalloproteinase 9, Animals, Genetically Modified, Dendritic Spines, Mice, Rats

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review