The DOI matches its Crossref record; 52 of the 52 references it cites were found in a registry or an index.
The Transcriptome and Metabolic Gene Signature of Protoplasmic Astrocytes in the Adult Murine Cortex
Open accessAbstract
Protoplasmic astrocytes are critically important to energy metabolism in the CNS. Our current understanding of the metabolic interactions between neurons and glia is based on studies using cultured cells, from which mainly inferential conclusions have been drawn as to the relative roles of neurons and glia in brain metabolism. In this study, we used functional genomics to establish the relative compartmentalization of neuronal and astrocytic metabolic pathways in the adult brain. To this end, fluorescence-activated cell sorting was used to directly isolate neurons and protoplasmic astrocytes from the cortex of adult mice. Microarray analysis showed that astrocytes and neurons each express transcripts predicting individual self-sufficiency in both glycolysis and oxidative metabolism. Surprisingly, most enzymes in the tricarboxylic acid (TCA) cycle were expressed at higher relative levels in astrocytes than in neurons. Mass spectrometric analysis of the TCA cycle intermediates confirmed that freshly isolated adult astrocytes maintained an active TCA cycle, whereas immuno-electron microscopy revealed that fine astrocytic processes encompassing synapses contained a higher density of mitochondria than surrounding cells. These observations indicate that astrocytes exhibit robust oxidative metabolism in the intact adult brain and suggest a prominent contribution of astrocytic metabolism to functional brain imaging, including BOLD (blood-oxygen level-dependent) functional magnetic resonance imaging signals.
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Cites
Cluster analysis and display of genome-wide expression patterns
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An Energy Budget for Signaling in the Grey Matter of the Brain
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Imaging Neuronal Subsets in Transgenic Mice Expressing Multiple Spectral Variants of GFP
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“This direct observation speaks to a number of inferential reports to the contrary, which have suggested that astrocytic metabolism is either predominantly (Schurr, 2006) or exclusively (Pellerin and Magistretti, 1994; Sibson et al., 1998; Magistretti et al., 1999; Attwell and Laughlin, 2001) glycolytic.”
BackgroundAstrocytes, from brain glue to communication elements: the revolution continues
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Astrocytes expressing ALS-linked mutated SOD1 release factors selectively toxic to motor neurons
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Astrocyte-mediated control of cerebral blood flow
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Self-citationWild-Type Nonneuronal Cells Extend Survival of SOD1 Mutant Motor Neurons in ALS Mice
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Cited by
Astrocytes: Biology and pathology
Sofroniew, M. V., & Vinters, H. V. (2010) · Acta Neuropathologica · doi:10.1007/s00401-009-0619-8 · Cited by 5,196
Zhang, Y., Chen, K., Sloan, S. A., Bennett, M. L., Scholze, A. R., O'Keeffe, S., Phatnani, H. P., Guarnieri, P., Caneda, C., Ruderisch, N., et al. (2014) · The Journal of Neuroscience · doi:10.1523/jneurosci.1860-14.2014 · Cited by 4,875
“Previous groups have performed transcriptome studies of one or more purified cell types in the brain (Dugas et al., 2006; Rossner et al., 2006; Lovatt et al., 2007; Cahoy et al., 2008; Doyle et al., 2008; Lau et al., 2008; Daneman et al., 2010; Wylie et al., 2010; Bracko et al., 2012; Friedrich et…”
BackgroundMethodMolecular dissection of reactive astrogliosis and glial scar formation
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Brain energy metabolism: focus on Astrocyte-neuron metabolic cooperation
Bélanger, M., Allaman, I., & Magistretti, P. J. (2011) · Cell Metabolism · doi:10.1016/j.cmet.2011.08.016 · Cited by 2,313
Verkhratsky, A., & Nedergaard, M. (2018) · Physiological Reviews · doi:10.1152/physrev.00042.2016 · Cited by 1,678
Self-citation
Metadata
- Authors
- Lovatt, D., Sonnewald, U., Waagepetersen, H. S., Schousboe, A., He, W., Lin, J. H.-C., Han, X., Takano, T., Wang, S., Sim, F. J., Goldman, S. A., & Nedergaard, M.
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2007-11-07
- Volume
- 27
- Issue
- 45
- Pages
- 12255-12266
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- OpenAlex
- W2022908550
- Cited by
- 489
- References
- 52
- FWCI
- 7.98
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: The Transcriptome and Metabolic Gene Signature of Protoplasmic Astrocytes in the Adult Murine Cortex. https://app.cochat.ai/papers/10.1523/jneurosci.3404-07.2007
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- Lovatt, D., Sonnewald, U., Waagepetersen, H. S., Schousboe, A., He, W., Lin, J. H.-C., Han, X., Takano, T., Wang, S., Sim, F. J., Goldman, S. A., & Nedergaard, M. (2007). The Transcriptome and Metabolic Gene Signature of Protoplasmic Astrocytes in the Adult Murine Cortex. The Journal of Neuroscience. https://doi.org/10.1523/jneurosci.3404-07.2007
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
